감마선(^60Co) 조사에 의한 항진균 세균의 돌연변이체 유도

정혜영,이영근,김재성,조규성,이영복 한국환경농학회 2002 한국환경농학회지 Vol.21 No.3

항진균 세균의 특성 및 기능변화 가능성을 조사하기 위하여 버섯폐배지, 온천수, 해조류 및 삼림토양으로부터 식물병원성 진균에 대한 8 종의 항진균 활성 균주를 분리하였고 감마선(^60Co)을 이용하여 LD_95 에 돌연변이체를 유도하였다. Bactllus circulans K1, Burkholderia gladioli K4와 Bacillussubtilis YSl은 12 종의 식물병원성 진균에 대go 항진균 활성을 보였다. 이들 균주의 방사선감수성 조사결과 B. gladioliK4는 감마선에 대한 높은 감수성을 보였으며, D_10 값은 0.11kGy 였다. 감마선에 의해 유도된 Kl-1004와 YSl-1009는Botryosphaeria dothidea에 대해 항진균 활성이 증가되었다. B.subtilis YS1의 돌연변이체인 YSl-1006과 YS1-1009는 tebuconazol과 copper hydroxide에 대해 농약 저항성을 나타냈다. SAR535, SAR5108 과 SAR5l18 돌연변이체는 야생형 균주인 Streptomyces sp. SAR01에 비해 5 종의 식물병원성 진균에 대해 항진균 활성이 없었다. 연구결과, 방사선을 이용하여 다양한 기능의 돌연변이체 유도가 가능하였다. 이를 이용하여 항진균 활성 관련 유전자 연구 및 균주개량이 가능할 것으로 사료된다. In order to evaluate the antifungal activity of bacteria against plant pathogenic fungi, 8 bacteria were isolated from mushroom compost, hot spring, seaweed, and forest soil and mutants from them were induced by LD_(95) gamma radiadon(^(60)Co). Bacillus circuluns K1, Burkholderia gladioli K4 and Bacillus subtilis YS1 showed wide antifungal spectrum against 12 kinds of plant pathogenic fungi. From the radiation sensitivity test, R gladioli K4 was very sensitive to gamma radiation and its D_(10) value was 0.11 kGy. Antifungal activities of B. circulans K1-1004 and B. subtilis YS1-1009, which were induced by the radiation of ^(60)Co increased against Botryosphaeria dothidea. The mutant strains, B. subtilis YS1-1006 and B. subtilis YS1-1009 were resistant to tebuconazole and copper hydroxide. SAR535, SAR5108, and SAR5118 mutated from Streptomyces sp. SAR01 were antifungal activity deficient mutants against 5 kinds of plant pathogenic fungi compared to wild strain, so that they could be supposed to be model strains for studying antifungal mechanism. It is suggested that various functional types of mutants could be induced by gamma radiation and applied usefully.

점용접부의 피로강도 평가에 관한 연구

鄭鎭成,金英勳,李龍福,朴永勤 弘益大學校 科學技術硏究所 1997 科學技術硏究論文集 Vol.8 No.-

Spot welding has been used in the sheet metal joining processes because of its high productivity and convenience. In this study, effects of welding conditions on the fatigue life of spot welded joint and prediction of its fatigue life based on fracture mechanics theory of spot welded joint have been investigated. Fatigue tests were conducted with the tensile-shear specimens using cold rolled steel sheets. Fatigue life of spot welded joint was predicted and compared with experimental results. The experimental results are summarized as follows : 1. The static strength increased due to the increase in welding current, but the fatigue strength decreased while welding current being excessively increased. Therefore, the fatigue strength of spot weld was not proportional to the static strength. 2. Fatigue life of spot welded joint cannot be predicted accurately using the prediction methods proposed by Pook nor Findley. Therefore, it is necessary to consider stress index parameter method proposed by Kurath. 3. It is found that FEM analysis is powerful approach to evaluate the fatigue life of tensile shear spot welded joints with different geometry.

동치미 유래 생균제로서 가능성이 있는 Lactobacillus sp. FF-3의 분리와 특성

정원복,서원석,차재영,조영수 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

생균제로서 가능성 있는 유산균 선별을 위해 발효식품인 동치미로부터 분리된 균주들의 내산성, 내담즙성, 내췌장액성 및 유해균 억제능을 조사하였다. 특히 분리된 균종 중에서 FF-3가 인공위액과 담즙산에 대한 높은 내성을 나타내었을 뿐만 아니라, Salmonella sp.과 Escherichia coli에 대하여 높은 항균 활성을 나타내었다. FF-3유래 박테리오신은 그람 양성 및 음성 세균에서 넓은 항균범위를 나타내었다. 본 연구에서 최종선별된 FF-3 균주들 16s rDNA sequence 동정한 결과 Lactobacillus sp.으로 확인되어 FF-3으로 명명하였다. For selection of lactic acid bacterial for probiotics, we have examined isolated strains from Korean Dongchimi to assess the acid, bile, and pancreatic tolerance and the growth inhibition on the pathogens, Expecially, a kind of isolated strains, FF-3 showed the highest resistancy to both of HCI and bile sale, as well as the highest inhibitory activities against Salmonella sp. and Escherichia coil. Further the bacteriocin of FF-3 showed relatively wide range of inhibition spectrum against gram positive and some gram negative bacterias. By using 16s rDNA sequencing method, FF-3 of the selected lactic acid bacteria were found to be identified as genus Lactobacillus.

香粧크림의 製造와 그效用

鄭魯華,朴英福 梨花女子大學校 藥學硏究所 1961 梨花藥學硏究 Vol.- No.2

에어로빅스 운동시 부상 유발 동작의 운동 역학적 분석

정철수,신인식,현무성,배성제,김복영,이기청 한국운동역학회 1995 한국운동역학회지 Vol.5 No.1

One of the reason that the aerobic dance is popular for korean women is that it is considered as a moderate aerobic exercise. However, high rate of aerobic dance related injuries have been reported among the participants. The purpose of this study was to estimate the impulsive load to the body while performing certain aerobic dance movement. Based on the preliminary questionairs on aerobic dance injury for 210 replyees, two aerobic dance movements including Run and High Kick were identified as high injury induced movements. The impulsive loads during aerobic dance estimated were joint angular acceleration and the vertical ground reaction force. The joint angular acceleration was determined using a 3-D cinematography technique, and the ground reaction force was measured with using an AMTI force platform system. It was founded that : the peak vertical ground reaction force 1738.7±171.2N, 1990.9±134.9N for Run, High Kick respectively. The peak vertical ground reaction force during High kick movement reached 3.7 body weight, which exceeded 3 body weight of force that can be experienced during jogging. These results indicates that although the aerobic dance movements look moderate means of exercise, certain movement ca produce highly impulsive look to the body and can aggravate injury when the movement is, kept repeated faster than the speed of healing process.

豚과 韓國人의 奇靜脈에 關한 比較解剖學的 硏究

鄭軸炫,盧永福 朝鮮大學校 文理科大學 1975 論文集 Vol.2 No.-

The authors studied on the comparative anatomical classification of the azygos veins in pig and Korean adults. The used materials were 78 pigs and Korean cadavers. The racial freqency and the various types of the azygos veins were recorded as followings. 1. TypeⅠcomprised 84.62% and type Ⅱ comprised 15.38% in pig. 2. The racial frequency of the hemiazygosveins comprised in Berkshire 26.9%, Hampshire 10.24%, Yorkshire 6.41%, Landrace 5.12% and in hybrid 35.8%. The racial frequency of the azygos veins compreised in Berkshire 5.12%, Hampshire 0.0%, Landrace 0.0% and in hybrid 10.25%. 3. TypeⅠcomprised 79.17%, type Ⅱ 12.5% and type Ⅲ 8.33% in the Korian radical frequency. 4. It was certifed that the azygos veins and hemiazygos veins were formed by the development of the postcardinal veins and the homologous development in phylogeny was presumed.

Aspergilli에 대한 Penicillia의 Mycoparasitism에 관하여

鄭圭永,李馥權,申炳湜 건국대학교 1978 論文集 Vol.8 No.1

The parasitisms of one fungus on another has been recognized by mycologists for one hundred years. As early as 1865 and 1870, de Bary described and named Piptocephalis freseniana and Cicinnobolus cesati and recognized them as mycoparasites. In these experiments, the light and electron microscopic study of the host-parasite relationships of Penicillium rugulosum and Asperqillus spp was made to investigate pathological histology and anatomical structure and similarities and differences between the haustoria of mycoparasites and parasites of vascular plants. The hosts were identified to be Asperqillus flavus (A-1) and Aspergillus oryae (A-2) and the parasite to be Penicillum rugulosum. The parasite (Penicillum rugulosum) was isolated from the host by method of Berry & Barnett and inoculated and cultured on the Synthetic medium (Czapek'a Dox ager), so the parasite is non obligate. The haustoria of the mycoparasite, Penicillium rugulosum, on Aspergillis flavus are similar to those of parasitic fungi on vascular plants. An electron-dense sheath bounded by a membrane surrounds the haustroail wall. The sheath membrane appears to be the invaginated host ectoplast. No collar is formed around the haustorium by the host wall.

완두 F_1세대의 조합능력 검정

정원복,오주성,황필성,황영현,신두철,한원영,김수경 동아대학교 교육대학원 2002 동아교육논총 Vol.28 No.-

완두의 이면교잡에 의한 F_1세대에 대한 조합능력을 검정한 결과는 다음과 같다. 9개 형질에 대한 GCA, SCA, RCA효과를 검정한 바 조합능력은 전 형질에서 GCA, SCA, RCA가 모두 유의하였고, 분지수·경직경·엽장·주당협수·협당립수·주당립수·100립중·주당립중은 GCA가 SCA보다 크고, 품종간 GCA효과는 고성종1호가 경직경과 100립중에서, 고성종2호가 경장·분지수·엽장에서, 김해종이 주당협수·주당립수·100립중·주당입중에서, 진해종이 협다립수에서 각각 정(+)으로 높았다. SCA효과에서 경장은 1×2(고성종1호×고성종2호)조합과 1×3(고성종1호×김해종)조합이, 분지수는 4×5(고성종3호×진해종)조합이, 경직경과 엽장은 1×3(고성종1호×김해종)조합이, 주당협수는 1×2(고성종1호×고성종2호)조합과 4×5(고성종3호×진해종)조합이, 협당립수는 1×2(고성종1호×고성종2호)조합·1×4(고성종1호×고성종3호)조합·2×3(고성종2호×김해종)조합이, 주당립수는 1×2(고성종1호×고성종2호)조합과 4×5(고성종3호×진해종)조합이, 100립중은 3×4(김해종×고성종3호)조합이, 주당립중은 1×2(고성종1호×고성종2호)조합·3×4(김해종×고성종3호)조합·4×5(고성종3호×진해종)조합이 각각 정으로 높았다. RCA효과에서 경장·엽장·주당협수·주당립중은 1×2(고성종1호×고성종2호)조합이, 주당협수·주당립수·100립중·주당립중은 2×4(고성종2호×고성종3호)조합이 각각 정으로 높았다. Five pea varieties in F₁ generation of the possible crosses among them were used to estimate the combining ability for nine characters, i. e., stem length, number of branches per plant, stem diameter, leaf length, number of pods per plant, number of seeds per pod, number of seeds per plant, 100-seed weight and seed weight per plant. Data for F₁ hybrid and parents were subjected to the analysis method proposed by Griffing. The results obtained were summarized as follows. Mean squares of general combining ability(GCA), specific combining ability(SCA) and reciprocal combining ability(RCA) were significant for all characters observed. Mean squares of GCA were higher than those of SCA and RCA in number of branches per plant, stem diameter, leaf length, number of pods per plant, number of seeds per pod, number of seeds per plant, 100-seed weight and seed weight and seed weight per plant. Variety Gimbae showed the highest GCA effect for number of pods per plant, number of seeds per plant and seed weight per plant and positively high effects of GCA were observed from variety Gosung#1 in stem diameter, 100-seed weight, and from variety Gosung#2 stem length, number of branches per plant, leaf length, from Jinhae in number of seeds per pod In SCA effects, hybrids in Gosung#1×Gosung#2 were wxhibited positively high for stem length, number of pods per plant, number of seeds per pod, number of seeds per plant and seed weight per plant, and high SCA effects for number of branches per plant, number of pods per plant, number of seeds per plant and seed weight per plant were found positively in Gosung#3×jinhae. In RCA effects, hybrids in Gosung#2×Gosung#3 were exhibited positively high for number of pods per plant, number of seeds per plant, 100-seed weight and seed weight per plant and high RCA effects for stem length, leaf length, number of pods per plant, and seed weight per plant were found positively in Gosung#1×Gosung#2.

과학 성취도에 영향을 미치는 전두엽 연합령의 기능에 대한 종단적 연구

김영신,권용주,박윤복,구수정,정완호 한국교원대학교 과학교육연구소 2000 청람과학교육연구논총 Vol.10 No.1

The purpose of this study was to longitudinally analyze influences of functional prefrontal lobe on science achievement in middle school students. In this longitudinal study, 47 students(male 19, female 27) were sampled in local area. The students were administered test of GALT(group assessment of logical thinking), CTST(classroom test of scientific test), FIT(figural intersection test), GEFT(group embedded figural intersection test), TOL(tower of London), WCST(Wisconsin card sorting test) at December 1997 and 1999. It is substitute science achievement for science grade each year. The resulted that prefrontal lobe was influenced upon science achievement. Science achievement statistically significant correlated with functional prefrontal lobe, specially logical thinking level, scientific reasoning, perseveration error. And, the development of functional prefrontal lobe functions did not only linear, but also each function has differ with development speed and period. School science classroom was suited high level students. Who has been grade C and D in first-grade, they did not improved. A first-grade achievement effect on science achievement. And also scientific reasoning ability effect on science achievement.

현대무용의 Leap 동작에서 숙련자와 비숙련자간의 운동학적 변인 비교 연구

김복영,배성제,정철수,이정은,임규찬 師範大學 體育硏究所 1992 서울大學校 體育硏究所論集 Vol.13 No.1

The purpose of this study was to compare kinematics of the skilled group with the corresponding of the unskilled group during the leap of the mordern dance and to investigate the effective movement patterns of the leap. The kinematic and temporal variables examined using the DLT(Direct Linear Transformation) method of three-dimensional cinematography were leap time, leap distance, leap height and body segment angles at the instants of take off, peak height and touch down. Four skilled and four unskilled groups were selected as the subjects. Two 16mm high speed cameras were used to monitor the subjects’movement patterns. T-test was used to determine if there is any significant (p<0.05) differences on kinematic variables between the skilled and unskilled groups. The conclusions obtained from the analyzed data were as follows; (1) There were not any significant differences between two groups for the angles of body segment at the instant of take off and leap distance. (2) At the instant of peak height, the leap angle and the left ankle angle for the skilled groups were larger than for unskilled groups. (3) At the instant of touch down, the trunk angle in the sagittal plane for the skilled groups were larger than for unskilled groups. (4) The flight time and height for the skilled groups were longer and higher than for the unskilled groups.