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        Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

        ( Xiaojian Ma ),( Chongde Wu ),( Huang Jun ),( Rongqing Zhou ),( Bi Shi ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.7

        The aim of this study was to investigate the microbial community of three tannery wastewater treatment plants (WWTPs) involved in nitrification by Illumina MiSeq sequencing. The results showed that highly diverse communities were present in tannery wastewater. A total of six phyla, including Proteobacteria (37-41%), Bacteroidetes (6.04-16.80), Planctomycetes (3.65- 16.55), Chloroflexi (2.51-11.48), Actinobacteria (1.91-9.21), and Acidobacteria (3.04-6.20), were identified as the main phyla, and Proteobacteria dominated in all the samples. Within Proteobacteria, Beta-proteobacteria was the most abundant class, with the sequence percentages ranging from 9.66% to 17.44%. Analysis of the community at the genus level suggested that Thauera, Gp4, Ignavibacterium, Phycisphaera, and Arenimonas were the core genera shared by at least two tannery WWTPs. A detailed analysis of the abundance of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) indicated that Nitrosospira, Nitrosomonas, and Nitrospira were the main AOB and NOB in tannery wastewater, respectively, which exhibited relatively high abundance in all samples. In addition, real-time quantitative PCR was conducted to validate the results by quantifying the abundance of the AOB and total bacteria, and similar results were obtained. Overall, the results presented in this study may provide new insights into our understanding of key microorganisms and the entire community of tannery wastewater and contribute to improving the nitrogen removal efficiency.

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        Adsorption characteristics of methylene blue on poplar leaf in batch mode: Equilibrium, kinetics and thermodynamics

        Xiuli Han,Xiaona Niu,Xiaojian Ma 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.4

        Adsorption characteristics of methylene blue (MB) from aqueous solution on natural poplar leaf were investigated. Batch experiments were carried out to study the effects of initial pH, contact time, adsorbent dosage, and initial MB concentration, salt concentration (Ca2+ and Na+) as well as temperature on MB adsorption. The optimum condition for adsorption was found at pH 6-9 and adsorbent dosage of 2 g L−1. The equilibration time was 240 min. The salt concentration had a negative effect on MB removal. The equilibrium data were analyzed with Langmuir, Freundlich and Koble-Corrigan isotherm models using nonlinear regression method. The adsorption process was more effectively described by Langmuir isotherm based on the values of the correlation coefficient R2 and chi-square statistic x2. The maximum monolayer adsorption capacity of poplar leaf from the Langmuir model was 135.35 mg g−1 at 293 K. The pseudo second order equation provided a better fit to experimental data in the kinetic studies. Intraparticle diffusion was involved in adsorption process, but it was not the only rate-controlling step. Thermodynamic quantities such as ΔG, ΔH and ΔS were calculated, indicating that the adsorption process was spontaneous and endothermic. Dye-adsorbent interactions were examined by FTIR and SEM analysis. The FTIR results suggested that there were hydroxyl and carboxyl groups on the surface of poplar leaf, which would make MB adsorption possible. The SEM images showed effective adsorption of MB molecules on the adsorbent surface.

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        Numerical Investigation on the Ultimate Compression Behavior of Un-strengthened and CFRP-strengthened Steel Tube with Random Pitting Corrosion Damage

        Lu Yao,Shaofeng Zhang,Hang Yu,Jiajing Xu,Xiaojian Cao,Yan Ma 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.11

        This paper mainly numerically investigates the ultimate compression strength of un-strengthened and CFRP-strengthened steel tubes with random pitting corrosion damage. First, a developed numerical method for steel tubes with random corroded pits is introduced in detail. The accuracy of a similar numerical method is verified through the corresponding experimental measurement and numerical prediction. Then, the ultimate axial compression behavior of steel tubes with random elliptical corroded pits is numerically investigated. Several influencing factors on the ultimate strength of corroded steel tubes under axial compression load are simulated and compared, including different random distributions, region size of corroded pits, number of pits, and depth of pits. Subsequently, the method for strengthening steel tubes by using CFRPs is studied and conducted on the corroded regions of steel tubes. The reliability of steel tubes strengthened by FRP is validated through experimental and numerical results. Finally, the residual compression performance and progressive damage mechanisms of CFRP-strengthened steel tubes are researched. The different parametric influences on the strengthened performance of FRPs are simulated, including layer direction, number of composite layers, and composite materials. The results demonstrate that FRP can improve the axial compression resistance of corroded steel tubes.

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