Kinetics of di-n-Butyl Phthalate Degradation by a Bacterium Isolated from Mangrove Sediment

( Xiang Rong Xu ),( Ji Dong Gu ),( Hua Bin Li ),( Xiao Yan Li ) 한국미생물생명공학회 2005 Journal of microbiology and biotechnology Vol.15 No.5

Properties of an AlGaN/AlN Distributed-Bragg-reflector Structure

Li-Li Zhang,Zhan-Hui Liu,Xiao-Gu Huang,Qing-Fang Li,Rong Zhang,Zi-Li Xie,Xiang-Qian Xiu 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.65 No.7

An AlGaN/AlN distributed-Bragg-reflector (DBR) structure with a high Al content was grown byusing plasma-assisted molecular beam epitaxy (PA-MBE). The properties of the sample were characterizedby using the transmission electron microscopy, high-resolution X-ray diffraction, atomicforce microscopy, and reflectivity spectrum measurements. The reciprocal space mapping analysisindicated that the strain in the AlGaN layers was partially relaxed. The morphology of the DBRexhibited a surface covered by grains (average size of about 130 nm), and the surface roughness wasabout 2 nm. The spectral measurements showed that the DBR structure presented a peak reflectivityof 68.8% at the center wavelength of 247 nm, which indicated that this DBR structure couldwork in the deep solar-blind UV region with acceptable reflectivity. However, the optical propertiesof the DBR structure were deteriorated by the fluctuation of the Al composition, non-uniformity ofthe layer thickness, the blurry, rough interface in the DBR structure, and so on.

Properties of a CdZnO/ZnO Multiple Quantum-Well Light-Emitting Diode

Zhan-Hui Liu,Li-Li Zhang,Qing-Fang Li,Rong Zhang,Zi-Li Xie,Xiang-Qian Xiu,Bin Liu 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.69 No.7

A CdZnO/ZnO multiple quantum-well light-emitting diode (LED) structure was successfully grown by using plasma-assisted molecular beam epitaxy on a p-GaN template that had been grown by using metal-organic chemical-vapor deposition on a c-sapphire substrate. The properties of the sample were characterized by using high-resolution X-ray diffraction, transmission electron microscopy, and temperature-dependent photoluminescence measurements. The light output performance of the CdZnO/ZnO QW LED device was also investigated in detail by using I-V and electroluminescence spectral measurements. The characterization showed that our CdZnO/ZnO QW LED structure had good crystalline quality and weaker carrier localization. Owing to the heterojunction structure, the I-V curve indicated that the LED device had a higher turn-on voltage and series resistance. The EL measurement demonstrated that for our LED device’s optoelectronic characteristic, the carrier-screening effect played the dominant role in the emission-energy blue-shift mechanism, and the broadening of the emission energy width was mainly ascribed to the band-filling effect. Without a special heat sinking, the L-I curve exhibited slight efficiency droop after 30 mA.

MiR-133b Acts as a Tumor Suppressor and Negatively Regulates TBPL1 in Colorectal Cancer Cells

Xiang, Kai-Min,Li, Xiao-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Introduction: MicroRNAs have emerged as post-transcriptional regulators that are critically involved in tumorigenesis. This study was designed to explore the effect of miRNA 133b on the proliferation and expression of TBPL1 in colon cancer cells. Methods: Human colon cancer SW-620 cells and human colon adenocarcinoma HT-29 cells were cultured. MiRNA 133b mimcs, miRNA 133b inhibitors, siRNA for TBPL1 and scrambled control were synthesized and transfected into cells. MiR-133b levels in cells and CRC tumor tissue was measured by real-time PCR. TBPL1 mRNA was detected by RT-PCR. Cell proliferation was studied with MTT assay. Western blotting was applied to detect TBPL1 protein levels. Luciferase assays were conducted using a pGL3-promoter vector cloned with full length of 3'UTR of human TBPL1 or 3'UTR with mutant sequence of miR-133b target site in order to confirm if the putative binding site is responsible for the negative regulation of TBPL1 by miR-133b. Results: Real time PCR results showed that miRNA 133b was lower in CRC tissue than that in adjacent tissue. After miR-133b transfection, its level was elevated till 48h, accompanied by lower proliferation in both SW-620 and HT-29 cells. According to that listed in http://www.targetscan.org, the 3'-UTR of TBPL1 mRNA (NM_004865) contains one putative binding site of miR-133b. This site was confirmed to be responsible for the negative regulation by miR-133b with luciferase assay. Further, Western blotting and immunohistochemistry both indicated a higher TBPL1 protein expression level in CRC tissue. Finally, a siRNA for TBPL1 transfection obviously slowed down the cell proliferation in both SW-620 and HT-29 cells. Conclusion: MiR-133b might act as a tumor suppressor and negatively regulate TBPL1 in CRC.

Propagation Properties of a Gaussian Beam from a High-NA Optical System in a Magneto-Optical Disk

Li-Chen Jia,Yu-Xiang Zheng,Liang-Yao Chen,Peng-Hui Mao,Rong-Jun Zhang 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.51 No.II

We have calculated the propagation properties of a Gaussian beam from a high-numerical-aperture (NA) optical system in a magneto-optical (MO) disk by combining an angular spectra analysis and optical matrix method. We obtained the optical and magneto-optical responses, the electric and magnetic field distributions, and the Joule loss profiles for the MO disk on which a Gaussian beam from different NA system was incident and compared the propagation properties of the Gaussian beam with those of a plane wave. The results show that the propagation properties of a Gaussian beam from an optical system with a high NA differ obviously from those of a plane wave, but a Gaussian beam with a larger spot diameter ({\it e.g.}, greater than 100 $\mu$m) can be regarded as a plane wave for the considered disk structure.

Evaluation of In-Vitro Dissolution and In-Vivo Absorption for Two Different Film-Coated Pellets of Clarithromycin

Xiang-rong Zhang,Xiao-yan Chen,Lian-Dong Hu,Xing Tang,San-Ming Li,Da-fang Zhong 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.8

The aim of this study was to compare two formulations of film-coated pellets containing clarithromycin after single oral dose study in healthy male volunteers. Two formulations with different coating polymers were prepared: formulation-1 (F-1) was prepared by incorporating three kinds of pH-dependent gradient-release coated pellets into capsules and formulation-2 (F-2) was prepared by coated with an insoluble semiosmotic film. Release profiles of filmcoated pellets were evaluated using paddle method under different conditions. Pharmacokinetic profiles of these formulations were obtained in three healthy male volunteers and compared to commercially available immediate release (IR) tablets. The relative bioavailability based on the AUC0-24 h was found to be 96.2% and 58.7% for F-1 and F-2 compared with IR, and the Tmax was delayed.

Evaluation of In-Vitro Dissolution and In-Vivo Absorption for Two Different Film-Coated Pellets of Clarithromycin

Zhang Xiang-rong,Chen Xiao-yan,Hu Lian-Dong,Tang Xing,Li San-Ming,Zhong Da-fang The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.8

The aim of this study was to compare two formulations of film-coated pellets containing c1arithromycin after single oral dose study in healthy male volunteers. Two formulations with different coating polymers were prepared: formulation-1 (F-1) was prepared by incorporating three kinds of pH-dependent gradient-release coated pellets into capsules and formulation-2 (F-2) was prepared by coated with an insoluble semiosmotic film. Release profiles of filmcoated pellets were evaluated using paddle method under different conditions. Pharmacokinetic profiles of these formulations were obtained in three healthy male volunteers and compared to commercially available immediate release (IR) tablets. The relative bioavailability based on the $AUC_{0-24h}$ was found to be $96.2\%\;and\;58.7\%$ for F-1 and F-2 compared with IR, and the $T_{max}$ was delayed.

An Oligonucleotide Microarray Bait for Isolation of Target Gene Fragments

Shi, Rong,Ma, Wen-li,Liu, Cui-Hua,Song, Yan-Bin,Mao, Xiang-Ming,Zheng, Wen-Ling Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.2

A new molecular-baiting method was studied by retrieving targeted gene fragments from an oligonucleotide microarray bait after hybridization. To make the microarray bait, 70-mer oligonucleotides that were designed to specifically represent the SSA1 gene of Saccharomyces cerevisiae were printed on the slide. Samples of the Saccharomyces cerevisiae mRNA were extracted and labeled by the RD-PCR (Restriction Display PCR) method using the Cy5-labelled universal primer, then applied for hybridization. The sample fragments that hybridized to the microarray were stripped, and the eluted cDNAs were retrieved and cloned into the pMD 18-T vector for transformation, plasmid preparation, and sequencing. BLAST searching of the GenBank database identified the retrieved fragments as being identical to the SSA1 gene (from 2057-2541bp). A new method is being established that can retrieve the sample fragments using an oligo-microarray-bait.

An Oligonucleotide Microarray Bait for Isolation of Target Gene Fragments

( Shi Rong ),( Ma Wen Li ),( Liu Cui Hua ),( Song Yan Bin ),( Mao Xiang Ming ),( Zheng Wen Ling ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.2

A new molecular-baiting method was studied by retrieving targeted gene fragments from an oligonucleotide microarray bait after hybridization. To make the microarray bait, 70-mer oligonucleotides that were designed to specifically represent the SSAl gene of Saccharomyces cerevisiae were printed on the slide. Samples of the Saccharomyces cerevisiae mRNA were extracted and labeled by the RD-PCR (Restriction Display PCR) method using the CyS-labelled universal primer, then applied for hybridization. The sample fragments that hybridized to the microarray were stripped, and the eluted cDNAs were retrieved and cloned into the pMD 18-T vector for transformation, plasmid preparation, and sequencing. BLAST searching of the GenBank database identified the retrieved fragments as being identical to the SSAl gene (from 2057-2541bp). A new method is being established that can retrieve the sample fragments using an oligo-microarray-bait.

Fitting the Nonlinear Systems Based on the Kernel Functions Through Recursive Search

Jimei Li,Cheng Wang,Feng Ding,Yingjiao Rong,Xiangli Li 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.6

Membership function identification is an important part of studying fuzzy control theory. Gaussian membership functions are widely used in the defuzzification processes, while the simple fuzzy processing reduces the dynamic characteristics of models. In order to reflect the dynamic performance of the nonlinear systems accurately, this paper introduces the idea of the multi-model control and fits a kernel function for the defuzzification processes by selecting the scheduling modes. Based on the gradient search, we present a least mean square (LMS) algorithm to solve the parameter estimation problem of the nonlinear systems. Considering the difficulty of determining the step sizes in the LMS algorithm, an overall stochastic gradient (O-SG) algorithm is deduced to obtain the optimal step size and estimate the unknown parameters. In order to improve the estimation accuracy, we introduce a forgetting factor into the O-SG algorithm to obtain the overall forgetting factor stochastic gradient (O-FFSG) algorithm. With the appropriate forgetting factors, the O-FFSG algorithm can effectively used for identifying the nonlinear systems. The performances of the proposed algorithms are tested by a numerical example.