Selaginella subvaginata (Selaginellaceae), a new spikemoss from China

Xian-Chun Zhang,Aleksandr Petrovich Shalimov,강종수,Meng-Hua Zhang 국립생물자원관 2020 Journal of species research Vol.9 No.3

Selaginella vaginata is a common montane species with broad distribution in China and the Himalaya region, and several species that are morphologically similar to S. vaginata are distributed in Asia. The taxonomic revision of S. vaginata and related species was performed by morphological comparison of leaves, strobili, and spores, and phylogenetic analysis. Based on these results, a new species, S. subvaginata, sp. nov., has been identified. Morphologically, S. subvaginata has intermediate form between S. vaginata and S. repanda, which differs mainly in its main stem being erect, dorsal leaves long-ciliolate on inner margin and outer margin denticulate or with 2-4 cilia at base (long-ciliolate on both inner and outer margins in S. vaginata, denticulate on both inner and outer margins in S. repanda), and acroscopic base of ventral leaves long ciliolate (sparsely long ciliolate in S. vaginata, short ciliolate to denticulate in S. repanda). Moreover, phylogenetic analysis using three chloroplast markers (rbcL, atpI, and psbA) revealed that S. subvaginata is a distinct species among the anisosporophyllous species clade in Selaginellaceae.

Polystichum (Dryopteridaceae) of Hainan Island, China

Zhang Xian Chun,김철환,문명옥,선병윤 한국식물분류학회 2005 식물 분류학회지 Vol.35 No.1

Polystichum chunii Ching is reported as a new record for the fern flora of Hainan Island. Taxonomy and nomenclature of the polystichum species from Hainan island, China are discussed.

Preparation and Properties of Green Environment-Friendly Drilling Polymer Mud

Zhang, Feng-Jun,Sun, Xian-Yang,Li, Xuan,Kong, Cui,Liu, Jin,Chen, Qian-Bao,Oh, Won-Chun Materials Research Society of Korea 2019 한국재료학회지 Vol.29 No.11

In this paper, a water-based green polymer mud is synthesized by simple compounding method. Effects of different kinds of tackifiers, their molecular weight on the viscosity of polymer mud and the effects of different fluid loss additives on mud fluid loss are studied. The results show that when polystyrene and anionic polyacrylamide with molecular weight of 8 ~ 10 million are used as the main thickening ingredient, polymer mud with high viscosity and high stability can be obtained. When the prepared polymer mud is formulated as NPAM: PEO: Hydroxypropyl cellulose(HPC) : Water = 42:10:10:100000 (unit: kg), the viscosity can reach 20.6 s, the filtration loss in 7.5 min is 24 mL, and the sand content is only 0.1 %. Compared with traditional bentonite mud, the green environment-friendly polymer mud has the advantages of small amount of waste, low environmental pollution, and low pulping cost, and can meet the construction needs for most topography and geomorphology drilling engineering.

Synthesis and Characterization of MoS₂/Graphene-TiO₂ Ternary Photocatalysts for High-Efficiency Hydrogen Production under Visible Light

Zhang, Feng-Jun,Kong, Cui,Li, Xuan,Sun, Xian-Yang,Xie, Wen-Jie,Oh, Won-Chun The Korean Ceramic Society 2019 한국세라믹학회지 Vol.56 No.3

Ternary MoS₂/graphene (G)-TiO₂ photocatalysts were prepared by a simple hydrothermal method. The morphology, phase structure, band gap, and catalytic properties of the prepared samples were investigated by X-ray diffraction, Raman spectroscopy, scanning electron microscopy, UV-vis spectrophotometry, and Brunauer-Emmett-Teller surface area measurement. The H₂ production efficiency of the prepared catalysts was tested in methanol-water mixture under visible light. MoS₂/G-TiO₂ exhibited the highest activity for photocatalytic H₂ production. For 5 wt.% and 1 wt.% MoS₂ and graphene (5MT-1G), the production rate of H₂ was as high as 1989 µmol^-1h^-1. The catalyst 5MT-1G showed H₂ production activity that was ~ 11.3, 5.6, and 4.1 times higher than those of pure TiO₂, 1GT, and 5MT, respectively. The unique structure and morphology of the MoS₂/G-TiO₂ photocatalyst contributed to its improved hydrogen production efficiency under visible light.

Genetic Variations in the HIF1A Gene Modulate Response to Adjuvant Chemotherapy after Surgery in Patients with Colorectal Cancer

Zhang, Yi,Wang, Peng,Zhou, Xing-Chun,Bao, Guo-Qiang,Lyu, Zhuo-Ming,Liu, Xiao-Nan,Wan, Shao-Gui,He, Xian-Li,Huang, Qi-Chao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Background: Hypoxia-inducible factor $1{\alpha}$ (HIF-$1{\alpha}$) plays an important role in regulating cell survival and angiogenesis, which are critical for tumor growth and metastasis. Genetic variations of HIF1A have been shown to influence the susceptibility to many kinds of human tumors. Increased expression of HIF-$1{\alpha}$ has also been demonstrated to be involved in tumor progression. However, the prognostic value of single nucleotide polymorphisms (SNPs) inthe HIF1A gene remains to be determined in most cancer types, including colorectal cancer (CRC). In this study, we sought to investigate the predictive role of HIF1A SNPs in prognosis of CRC patients and efficacy of chemotherapy. Materials and Methods: We genotyped two functional SNPs in HIF1A gene using the Sequenom iPLEX genotyping system and then assessed their associations with clinicopathological parameters and clinical outcomes of 697 CRC patients receiving radical surgery using Cox logistic regression model and Kaplan Meier curves. Results: Generally, no significant association was found between these 2 SNPs and clinical outcomes of CRC. In stratified analysis of subgroup without adjuvant chemotherapy, patients carrying CT/TT genotypes of rs2057482 exhibited a borderline significant association with better overall survival when compared with those carrying CC genotype [Hazard ratio (HR), 0.47; 95% confidence interval (95% CI): 0.29-0.76; P < 0.01]. Moreover, significant protective effects on CRC outcomes conferred by adjuvant chemotherapy were exclusively observed in patients carrying CC genotype of rs2057482 and in those carrying AC/CC genotype of rs2301113. Conclusions: Genetic variations in HIF1A gene may modulate the efficacy of adjuvant chemotherapy after surgery in CRC patients.

Antimicrobial Constituents from Fruits of Ailanthus Altissima SWINGLE

Chun-Chao Zhao,Jian-Hua Shao,Xian Li,Jing Xu,Peng Zhang 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.10

A new naturally occurring sterol, compound 5, and six known stigmasterols were isolated from fruits of Ailanthus altissima Swingle by repeated column chromatography and RP-HPLC. Their structures were identified as, 5α-stigmastane-3,6-dione (1), 3β-hydroxystigmast-5-en-7-one (2), stigmast-5-ene-3β, 7α-diol (3), 6α-hydroxystigmast-4-en-3-one (4), 5α-stigmastane-3β, 6β- diol (5), stigmast-4-ene-3β, 6α-diol (6), stigmast-5-ene-3β, 7α, 20ξ-triol (7) by spectral analysis and comparison with the published data. These compounds have not been reported from genus Ailanthus, whereas compound 7 was identified by NMR for the first time. In addition, the 95% ethanol extract and compounds from the fruits of Ailanthus altissima SWINGLE were assayed for in vitro antimicrobial activity. The extract was potent active against the assayed bacteria while compounds 3 and 7 exhibited moderate activity.

RPSA Gene Mutants Associated with Risk of Colorectal Cancer among the Chinese Population

Zhang, Shan-Chun,Jin, Wen,Liu, Hui,Jin, Ming-Juan,Chen, Ze-Xin,Ding, Zhe-Yuan,Zheng, Shuang-Shuang,Wang, Li-Juan,Yu, Yun-Xian,Chen, Kun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

The primary aim of this study was to evaluate the relationship of single nucleotide polymorphisms (SNPs) in ribosomal protein SA (RPSA) gene with colorectal cancer (CRC). A case-control study including 388 controls and 387 patients with CRC was conducted in a Chinese population. Information about socio-demography and living behavior factors was collected by a structured questionnaire. Three SNPs (rs2133579, rs2269349, rs7641291) in RPSA gene were genotyped by Illumina SnapShot method. Multiple logistic regression models were used for assessing the joint effects between tea consumption and SNPs on CRC. The subjects with rs2269349 CC genotype had a decreased risk for CRC (OR=0.60; 95%CI = 0.37-0.99), compared with TT/CT genotype after adjustment for covariates. A similar association of rs2269349 with rectal cancer was observed (OR=0.49; 95%CI=0.24-1.00). Further analyses indicated that this SNP could modify the protective effect of tea drinking on CRC. Among the subjects with rs2269349 TT/CT or rs2133579 AA/GA, there was a marginal significantly lower risk of CRC (OR and 95%CI: 0.63 and 0.39-1.01 for rs2269349; 0.64 and 0.40-1.02 for rs2133579) in tea-drinking subjects in comparison to non-tea-drinking subjects. Mutants in the RPSA gene might be associated with genetic susceptibility to CRC and influence the protective effect of tea consumption in the Chinese population.

Antimicrobial Constituents from Fruits of Ailanthus Altissima SWINGLE

Zhao Chun-Chao,Shao Jian-Hua,Li Xian,Xu Jing,Zhang Peng The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.10

A new naturally occurring sterol, compound 5, and six known stigmasterols were isolated from fruits of Ailanthus altissima Swingle by repeated column chromatography and RP-HPLC. Their structures were identified as, 5${\alpha}$-stigmastane-3,6-dione (1), 3${\beta}$-hydroxystigmast-5-en-7-one (2), stigmast-5-ene-3${\beta}$, 7${\alpha}$-diol (3), 6${\alpha}$-hydroxystigmast-4-en-3-one (4), 5${\alpha}$-stigmastane-3${\beta}$, 6${\beta}$-diol (5), stigmast-4-ene-3${\beta}$, 6${\alpha}$-diol (6), stigmast-5-ene-3${\beta}$, 7${\alpha}$, 20$\xi$-triol (7) by spectral analysis and comparison with the published data. These compounds have not been reported from genus Ailanthus, whereas compound 7 was identified by NMR for the first time. In addition, the $95\%$ ethanol extract and compounds from the fruits of Ailanthus altissima SWINGLE were assayed for in vitro antimicrobial activity. The extract was potent active against the assayed bacteria while compounds 3 and 7 exhibited moderate activity.

Aberrant Methylation of RASSF2A in Tumors and Plasma of Patients with Epithelial Ovarian Cancer

Wu, Yu,Zhang, Xian,Lin, Li,Ma, Xiao-Ping,Ma, Ying-Chun,Liu, Pei-Shu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Objective: The tumor suppressor gene, Ras-association domain family (RASSF)2A, is inactivated by promoter hypermethylation in many cancers. The current study was performed to evaluate the methylation status of RASSF2A in epithelial ovarian cancer (EOC) tissues and plasma, and correlations with gene expression and clinicopathologic characteristics. Method: We detected methylation of the RASSF2A gene in tissues and corresponding plasma samples from 47 EOC patients and 14 patients with benign ovarian tumors and 10 with normal ovarian tissues. The methylation status was determined by methylation-specific PCR while gene expression of mRNA was examined by RT-PCR. The EOC cell line, SKOV3, was treated with 5-aza-2'-deoxycytidine (5-azadC). Results: RASSF2A mRNA expression was significantly low in EOC tissues. The frequency of aberrant methylation of RASSF2A was 51.1% in EOC tissues and 36.2% in corresponding plasma samples, whereas such hypermethylation was not detected in the benign ovarial tumors and normal ovarian samples. The expression of RASSF2A mRNA was significantly down-regulated or lost in the methylated group compared to the unmethylated group (p<0.05). After treatment with 5-aza-dC, RASSF2A mRNA expression was significantly restored in the Skov3 cell line. Conclusion: Epigenetic inactivation of RASSF2A through aberrant promoter methylation may play an important role in the pathogenesis of EOC. Methylation of the RASSF2A gene in plasma may be a valuable molecular marker for the early detection of EOC.

New Materials of the Hymenophyllaceae from Hainan Island, China

선병윤,문명옥,Xian Chun Zhang,Bao Dong Liu,김철환 한국식물분류학회 2005 식물 분류학회지 Vol.35 No.3

During a recent trip to Hainan Island of China, we collected some filmy ferns from Diaoluo Mountain in the southeastern of the island. Trichomanes subclathratum turns out to be a new record to the Hainan fern flora, and also represent new record to China, and Mecodium riukiuense, so far has been unaware to Chinese pteridologists is also recorded here.