OB-12 : Expansion by basic fibroblast growth factor of non-enzymatically isolated perivascular cells from human umbilical cord

( Sunghun Na ),( Jong Yun Hwang ),( Hyang Ah Lee ),( Songyi Kook ),( Jung Hwa Ko ),( Dong Hun Lee ),( Seok Ho Hong2 ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: Recently, perivascular cells(PVCs) around blood vessels turn out to be the origin of all MSCs and can be isolated from various tissues based on the expression of CD146 and recent evidence indicates that PVCs possess the greater proliferation and differentiation potential than bone marrow- derived MSCs. Therefore, efficient isolation and expansion of PVCs from various fetal and adult tissues is an essential tool for providing sufficient amount of PVCs for more than one treatment. In this study, human umbilical cord blood vessel present in the PVC can be efficiently separated and cultured to develop methods, and further the efficiency of proliferation and for improving the reproduction performance evaluation of various growth factors through the limitations of the existing MSCs the foundation wants more appropriate and effective treatment of the cell material that propose new alternatives. 방법: This study was approved by the Institutional Review Board of Kangwon National University Hospital Authority and in 2013, Kangwon National University Hospital 13 peoples who visited a normal pregnancy after cesarean section consent discarded umbilical cord was used. In harvested human umbilical cord, the two of umbilical arteries were separated. One umbilical artery was isolated PVCs by non-enzymatic isolation( NE) method and another umbilical artery was isolated PVCs by collagenase(COL) method. To confirm that cultured PVCs were the stem cells was observed by adipogenic differentiation, osteogenic differentiation, chonrogenic differentiation. Furthermore, proliferation of PVCs were compared by treatment of growth factors, flow cytometry, immunocytochemistry, Fluorescence activated cell sorting(FACS) using the method of immune phenotype was compared with conventional enzyme treatment. 결과: While both methods displayed similar patterns or levels of phenotypic expression and growth rate, NE method had an increased frequency of CD146(+) population compared to COL method over passage. We also found that supplementation of basic fibroblast growth factor(bFGF) significantly enhanced PVC proliferation by driving the cells into S phase and increased a proportion of Stage-specific embryonic angtigen(SSEA)-4 population within PVC cultures without alteration of other immunophenotype. 결론: Our findings suggest that a combination of NE method and bFGF supplementation can be an epoch-making way to isolate more homogeneous PVC population from human umbilical cord, which will enhance the utility of PVCs for cell therapy for various diseases.

[학부생논문] Dirtycow 취약점 기반 vold 권한탈취를 이용한 안드로이드 사용자 데이터 초기화

나성훈(Na SungHun),석호식(Seok Ho-Sik) 한국정보과학회 2017 한국정보과학회 학술발표논문집 Vol.2017 No.12

Associations of night shift status during pregnancy with birth outcomes

Se Jin Lee(Se Jin Lee),Chorong Kim(Chorong Kim),Eun Ju Lee(Eun Ju Lee),Myoung-Nam Lim(Myoung-Nam Lim),Jeeyoung Kim(Jeeyoung Kim),Sunghun Na(Sunghun Na),Woo Jin Kim(Woo Jin Kim),Ko-CHENS study group(Ko 한국환경보건학회 2022 한국환경보건학회 학술대회 자료집 Vol.2022 No.5

Paracrine influence of human perivascular cells on the proliferation of adenocarcinoma alveolar epithelial cells

Eunbi Kim,Sunghun Na,Borim An,Se-Ran Yang,Woo Jin Kim,Kwon-Soo Ha,Eun-Taek Han,Won Sun Park,Chang-Min Lee,Ji Yoon Lee,Seung-Joon Lee,Seok-Ho Hong 대한생리학회-대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.2

Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-1α and K562)<i> in vitro</i> using Transwell^Ⓡ co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-1α and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.

Paracrine influence of human perivascular cells on the proliferation of adenocarcinoma alveolar epithelial cells

Kim, Eunbi,Na, Sunghun,An, Borim,Yang, Se-Ran,Kim, Woo Jin,Ha, Kwon-Soo,Han, Eun-Taek,Park, Won Sun,Lee, Chang-Min,Lee, Ji Yoon,Lee, Seung-Joon,Hong, Seok-Ho The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.2

Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-$1{\alpha}$ and K562) in vitro using $Transwell^{(R)}$ co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-$1{\alpha}$ and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.

Vacuum extraction vaginal delivery: current trend and safety

( Jihan Jeon ),( Sunghun Na ) 대한산부인과학회 2017 Obstetrics & Gynecology Science Vol.60 No.6

Operative vaginal birth retains an important role in current obstetric practice. However, there is an increasing trend in the rate of cesarean section in Korea. Surgical delivery is more advantageous than cesarean section, but the rate of operative vaginal delivery is decreasing for various reasons. Furthermore, there is no unified technique for vacuum extraction delivery. In this context, this review was performed to provide details of the necessary conditions, techniques, benefits, and risks of operative vaginal delivery. Future research should focus on overcoming the limitations of operative vaginal delivery.

Recent Trend about Pregnant Women with Suspected or Confirmed Coronavirus Disease 2019 (COVID-19) Infection

( Se Jin Lee ),( Sunghun Na ) 대한주산의학회 2020 Perinatology Vol.31 No.1

WHO에서 2020년 3월 11일 COVID-19에 대한 세계적대유행(pandemic)을 선언한 이후 이에 대한 전 세계적 관심이 높아지고 있으며, 3월 14일 기준 현재까지 127개국에서 142,491명의 확진자와 5,388명의 사망자가 보고되었다. 이후 산모의 감염사례가 중국에서 보고되고 있으며, 최근 국내에서도 산모의 감염 사례 및 분만 사례가 있었다. 아직 COVID-19에 대한 치료제나 백신은 발견되지 않았으며, 상대적으로 질병에 대하여 고위험군인 산모의 처치에 대한 통일된 국내 지침은 없는 상태이다. 최근 미국 질병통제예방센터(Centers for Disease Control and Prevention), 미국 모체태아의학회(Society for Maternal-Fetal Medicine), 세계산부인과초음파학회(International Society of Ultrasound in Obstetrics and Gynecology) 및 영국산부인과학회(Royal College of Obstetricians and Gynaecologists)에서 여러 권고사항을 발표하고 있다. 현재까지는 COVID-19에 대한 데이터가 부족하지만 같은 코로나바이러스 감염인 중동 호흡기 증후군(MERS) 및 중증 급성 호흡기 증후군(SARS)과 비슷한 형태일 것으로 예상하고 이에 준하여 대비할 수 있다. COVID-19에 대하여 분만 시 수직 감염의 증거는 없으며, 모유 또한 바이러스가 검출되지 않았다. 분만 방법은 상황에 따라 고려되어야 하지만 음압병실에서 완벽한 개인 보호구를 착용한 채로 질식분만도 가능하다. 출생 후 신생아는 산모와 분리되고 COVID-19 의심 환자로 격리되어야 하며 추적검사를 해야 한다. 앞으로 더 많은 연구를 통하여 코로나바이러스와 관련된 산모의 관리에 대한 임상지침이 만들어져야 할 것이다. Since the World Health Organization declared the pandemic of Coronavirus Disease 2019 (COVID-19) on March 11, there has been increasing worldwide interest in it. Since then, cases of maternal infection have been reported in China, and recent cases of maternal infection and delivery have also occurred in Korea. No cure or vaccine for COVID-19 has been found, and there is no unified domestic guideline for the treatment of relatively high-risk mothers against disease. Several recommendations from the Centers for Disease Control and Prevention, the American Society for Maternal-Fetal Medicine, the International Society of Ultrasound in Obstetrics and Gynecology, and Royal College of Obstetricians and Gynaecologists were suggested. So far, data on COVID-19 is scarce, but it is expected to be similar to the same coronavirus infections as a middle east respiratory syndrome and severe acute respiratory syndrome and can be prepared accordingly. For COVID-19, there was no evidence of vertical transmission at delivery, and the virus was not detected in breast milk. The delivery method should be considered depending on the situation, but vaginal delivery is also possible and should be performed with complete personal protective equipment in a negative-pressure isolation room. After birth, the newborn should be separated from the mother, and the newborn should be isolated as a suspected COVID-19 infection and followed up. In the future, further research should develop clinical guidelines for maternal care for COVID-19.

Ultrasonographic ovarian mass scoring system for predicting malignancy in pregnant women with ovarian mass

( Se Jin Lee ),( Hye Rim Oh ),( Sunghun Na ),( Han Sung Hwang ),( Seung Mi Lee ) 대한산부인과학회 2022 Obstetrics & Gynecology Science Vol.65 No.1

During routine antenatal ultrasound examinations, an ovarian mass can be found incidentally. In clinical practice, the differential diagnosis between benign and malignant ovarian masses is essential for planning further management. Ultrasound imaging has become the most popular diagnostic tool during pregnancy, with the recent development of ultrasonography. In non-pregnant women, several methods have been used to predict malignant ovarian masses before surgery. The International Ovarian Tumor Analysis (IOTA) group reported several scoring systems, such as the IOTA simple rules, IOTA logistic regression models, and IOTA assessment of different NEoplasias in the adneXa. Other researchers have also evaluated the malignancy of ovarian masses before surgery using scoring systems such as the Sassone score, pelvic mass score, DePriest score, Lerner score, and Ovarian-Adnexal Reporting and Data System. These researchers suggested specific features of ovarian masses that can be used for differential diagnosis, including size, proportion of solid tissue, papillary projections, inner wall structure, locules, wall thickness, septa, echogenicity, acoustic shadows, and presence of ascites. Although these factors can also be measured in pregnant women using ultrasound, only a few studies have applied ovarian scoring systems in pregnant women. In this article, we reviewed various scoring systems for predicting malignant tumors of the ovary and determined whether they can be applied to pregnant women.

Overview of Malignancy in Pregnancy

( Yung-taek Ouh ),( Geum Joon Cho ),( Sunghun Na ) 대한주산의학회 2020 Perinatology Vol.31 No.4

The incidence of cancer during pregnancy is increasing although it is relatively rare, which is expected to rise with an increase in the maternal age of the pregnancy. Due to the ambiguity of the symptoms, the diagnosis of cancer is often delayed. Diagnosis of cancer in pregnancy needs complex management issues that balance the benefit of treatment for the mother and harm for the fetus. Every patient should be personalized with a multidisciplinary system of obstetricians, gynecologists, oncologists, radiologists, and neonatal specialists and it should assist patients in making informed decisions about the best diagnosis and management for mother and fetus. Radiotherapy, chemotherapy, and surgery could be provided as a management option. In most cancer, pregnancy did not seem to have an adverse effect on maternal prognosis. In this article, we review various perspectives on the diagnosis and treatment of cancer during pregnancy as well as the prognosis of cancer in pregnancy.

Clinical Practice Guidelines for Prenatal Aneuploidy Screening and Diagnostic Testing from Korean Society of Maternal-Fetal Medicine: (2) Invasive Diagnostic Testing for Fetal Chromosomal Abnormalities

Lee Ji Yeon,Kwon Ji Young,Na Sunghun,Choe Seung-Ah,Seol Hyun-Joo,Kim Minhyoung,김민아,Park Chan-Wook,Kim Kunwoo,Ryu Hyun Mee,Hwang Han-Sung,Shim Jae-Yoon 대한의학회 2021 Journal of Korean medical science Vol.36 No.4

The Korean Society of Maternal Fetal Medicine proposed the first Korean guideline on prenatal aneuploidy screening and diagnostic testing, in April 2019. The clinical practice guideline (CPG) was developed for Korean women using an adaptation process based on good-quality practice guidelines, previously developed in other countries, on prenatal screening and invasive diagnostic testing for fetal chromosome abnormalities. We reviewed current guidelines and developed a Korean CPG on invasive diagnostic testing for fetal chromosome abnormalities according to the adaptation process. Recommendations for selected 11 key questions are: 1) Considering the increased risk of fetal loss in invasive prenatal diagnostic testing for fetal genetic disorders, it is not recommended for all pregnant women aged over 35 years. 2) Because early amniocentesis performed before 14 weeks of pregnancy increases the risk of fetal loss and malformation, chorionic villus sampling (CVS) is recommended for pregnant women who will undergo invasive prenatal diagnostic testing for fetal genetic disorders in the first trimester of pregnancy. However, CVS before 9 weeks of pregnancy also increases the risk of fetal loss and deformity. Thus, CVS is recommended after 9 weeks of pregnancy. 3) Amniocentesis is recommended to distinguish true fetal mosaicism from confined placental mosaicism. 4) Anti-immunoglobulin should be administered within 72 hours after the invasive diagnostic testing. 5) Since there is a high risk of vertical transmission, an invasive prenatal diagnostic testing is recommended according to the clinician's discretion with consideration of the condition of the pregnant woman. 6) The use of antibiotics is not recommended before or after an invasive diagnostic testing. 7) The chromosomal microarray test as an alternative to the conventional cytogenetic test is not recommended for all pregnant women who will undergo an invasive diagnostic testing. 8) Amniocentesis before 14 weeks of gestation is not recommended because it increases the risk of fetal loss and malformation. 9) CVS before 9 weeks of gestation is not recommended because it increases the risk of fetal loss and malformation. 10) Although the risk of fetal loss associated with invasive prenatal diagnostic testing (amniocentesis and CVS) may vary based on the proficiency of the operator, the risk of fetal loss due to invasive prenatal diagnostic testing is higher in twin pregnancies than in singleton pregnancies. 11) When a monochorionic twin is identified in early pregnancy and the growth and structure of both fetuses are consistent, an invasive prenatal diagnostic testing can be performed on one fetus alone. However, an invasive prenatal diagnostic testing is recommended for each fetus in cases of pregnancy conceived via in vitro fertilization, or in cases in which the growth of both fetuses differs, or in those in which at least one fetus has a structural abnormality. The guidelines were established and approved by the Korean Academy of Medical Sciences. This guideline is revised and presented every 5 years.