Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

구내방사선사진의 프랙탈 분석을 이용한 골다공증 예측

박금미,정연화,나경수 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.1

Purpose : The purpose of this study was to investigate whether the fractal dimension and radiographic image brightness of periapical radiograph were useful in predicting osteoporosis. Materials and Methods : Ninety-two postmenopausal women were classified as normal, osteopenia and osteoporosis group according to the bone mineral density of lumbar vertebrae and periapical radiographs of both mandibular molar areas were taken. The ROIs of 358 areas were selected at periapical and interdental areas and fractal dimension and radiographic image brightness were measured. Results : The fractal dimension in normal group was significantly higher than that in osteoporosis group at periapical ROI (P<0.05). The radiographic image brightness in normal group was higher than that in osteopenia and osteoporosis group. There was significant difference not only between normal and osteopenia group (P<0.05) but also within osteopenia and osteoporosis group (P<0.01) at periapical ROI. Significant difference was observed not only between normal and osteopenia group but also between normal and osteoporosis group at interdental ROI (P<0.01). Positive linear relationship was weakly shown at Pearson correlation analysis between fractal dimension and radiographic image brightness. BMD significantly correlated with fractal dimension at periapical ROI (P<0.01), and BMD and radiographic image brightness significantly correlated at both periapical and interdental ROIs (P<0.01). Conclusion : This study suggests that the fractal dimension and radiographic image brightness of periapical ROI may predict BMD.

동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

GLE의 영어 교과과정

박상수,정윤걸 釜山外國語大學校 語文學硏究所 1999 外大語文論集 Vol.14 No.-

The students who study in GLE(=Globalized and Localized experts) program must have a good command of English, especially in speaking and listening when they complete whole courses. In order to achieve this aim successfully the English curriculum for the GLE should be organized systematically as well as the intellectual faculties of the students, teaching methods and the beginning age of English teaming should be considered seriously We researched the learning theories of a foreign language and the four functional skills of language and proposed a few hints that the students in the GLE keep in mind while they learn English. The results obtained from this paper are summarized as follows: 1. The students who study in the GLE can effectively improve the ability of the command of English not through the way of language acquisition but through the way of language learning because they began to learn English at middle school days. In fact at the age of over 12 or 13 the language acquisition faculty has grown and consequently the value of linguistic parameters has already been set. 2. When the students in the GLE learn English, the emphasis should be laid on the improvement of speaking and listening ability primarily and then reading and writing ability should be developed. 3. It is necessary that the students understand the British and American social and cultural properties fully. As a particular language is the product of the social and cultural life of a certain language group, language learning always includes the understanding of the social and cultural properties of the people who use the target language.

재발성 다발성 연골염

박용범,이시훈,정상윤,이수곤,홍순원,김정민,이창률 대한내과학회 2001 대한내과학회지 Vol.61 No.3

동물의 장에서 분리한 Enterococcus sp.의 특성 및 분말화

박종진,변정수,조윤경,홍승서,이현수 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

의약품 및 동물약품으로 사용되고 있는 분말유산균을 개발하고자 동물의 장에서 산성 pH 및 담즙산에 대한 내성이 강하고 대장균 생육억제력이 좋은 Enterococcus faecium L20을 분리 동정하고 이를 이용하여 고농도의 생균이 함유된 분말 유산균을 제조하였다. 분리된 균주는 pH 3.0에서 90% 이상의 내성과 0.3% 담즙산이 함유된 배지에서 100%의 내성을 나타냈으며 MRS 배지에서 대장균과 혼합배양시 24시간 이내에 대장균을 사멸시켰다. 분리된 균주를 산업용배지에서 배양한 후 동결건조시켜 분말을 만들었을 때 생균수는 5.0×0^11/g 이상이었다. 이것은 18℃에 보관하였을 경우 11개월 동안 80%의 생존율을 나타내었다. In order to develop a lactic acid bacterial powder which can be used as a probiotic for human and animal, a lactic acid bacteria which has high resistance against low pH and ox-gall, and shows a good growth inhibition against low pH and ox-gall, and shows a good growth inhibition against E. coli, was isolated from an animal intestine and characterized. The isolated strain was identified as Enterococcus faecium. It had more than 90% of survival at low pH for 2 hours and almost 100% of survival in the presence of 0.3% ox-gall. When co-cultured with E. coli in MRS broth, all of the E. coli cells were killed within 24 hours. The final powdered product of the isolated strain was manufactured after a freeze drying process suing an industrial media, and then checked its stability. Its storage stability was 80% for 11 months at 18℃.

인공위성 자료에 의한 홍수흔적분석의 타당성 검증에 관한 연구

박병욱,최윤수 안성산업대학교 1997 論文集 Vol.29 No.2

The objective of this paper is to evaluate methodology that uses satellite data for analysis of flood damaged area. We performed land cover classification using satellite data that were acquired at two weeks after the floodwater recession, and verified accuracy by comparison with the analyzed output to field survey results. The results show that analyzed output are corresponded with field survey very well. and also, some areas that had been missed in field survey were found. Therefore we can prove that the suggested method is more accurate and effective for mapping inundated areas of floodplains than field survey that would be held a few month later.

관여도에 따른 TV 광고의 컬러 표현 방법이 인지효과 및 광고반응에 미치는 영향

박현수,이윤철 한국방송광고공사 2008 광고연구 Vol.0 No.80

The purpose of this study is to determine how color-expressing methods in TV advertisements affect cognitive effects and advertising responses according to involvement. The color-expressing methods were classified into color advertisements, partial color advertisements, and blackand-white advertisements; indexes for advertising responses were limited to good quality perception for a product, attitudes toward the advertisement, attitudes toward the brand, attitudes toward the sponsor, and purchasing intention. College students participated in this experiment were divided into three experimental groups, each of which consisted of 90 students. The three experimental groups were divided according to different kinds of color (blackand-white, color, partial color) and involvement (high- or low-involvement). The results demonstrated that partial-color advertisements were effective in cognitive effects, compared with color advertisements, as partialcolor ones showed statistically significant differences in all measurements. The high-involvement group perfectly supported the hypothesis that there would no difference in advertising responses by the kinds of color, while the low-involvement group supported the hypothesis that there were more positive advertising responses to black-and-white and partial-color advertisements than to color advertisements. There was a significant difference in purchasing intention between low-involvement partial-color advertisements and low-involvement color advertisements.

비소성 준설토의 침강-압밀 거동 특성

박윤균,박병수,정길수,유남재 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.A

A series of column test with a silty marine soil mixed with Jumunjin Standard Sand were performed to investigate the characteristics of settling and consolidation of non-plastic dredged soils. Column tests were carried out by using the separable column to measure the grain size distribution of consolidated layer. Column tests were performed with changing the mixing ratio of Jumunjin Standard Sand to the silty marine soil, initial water content of slurry and initial height of slurry. Height of interface of slurry was monitored during tests and grain size distribution tests were carried out after finishing tests. Influencing factors on the particle segregation, eventually to the characteristics of settling and consolidation of non-plastic soil, were analyzed on the thesis of test results. As results of column tests, the mixing ratio of sand to the silty marine soil and the initial water content of slurry were known to affect the characteristics of settling and consolidation resulted in significant particle segregation of slurry. Initial height of slurry was found not to affect seriously to particle segregation.

푸들에서 발생한 악성혼합유선종양

박수진,조성환,류시윤,손화영 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 2cm sized solid mass was observed in the right inguinal lesion of a 10-years old poodle bitch. Radiological finding in the mass showed higher density than that of soft tissue and thoracic radiograph showed pulmonary interstitial pattern. Gross findings appeared a yellowish-brown solid mass containing cysts filled with brown colored mucoid fluids in the udder. And the lung showed multiple nodules scattered throughout the pulmonary parenchyma. In light microscopic finding, mass contained neoplastic proliferation of glandular epithelial and myoepithelial cells and showed metaplstic lesions of these cells to chondrous tissue. Glandular epithelial cells were undifferentiated and polygonal myoepithelial cells were frequently embedded in or surrounded by a pink to light blue mucinous matrix. Tumor cells of the pulmonary nodule had resemblance to neoplastic proliferation of glandular epithelial and myoepithelial cells observed in the mammary gland mass. Based on the gross and microscopic findings, the mass was diagnosed as malignant mixed tumor with metastasis to the lung.