Influence of Varying Degree of Salinity-Sodicity Stress on Enzyme Activities and Bacterial Populations of Coastal Soils of Yellow Sea, South Korea

( Siddikee Md. Ashaduzzaman ),( Sherlyn C. Tipayno ),( Ki Yoon Kim ),( Jong Bae Chung ),( Tong Min Sa ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.4

To study the effects of salinity-sodicity on bacterial population and enzyme activities, soil samples were collected from the Bay of Yellow Sea, Incheon, South Korea. In the soils nearest to the coastline, pH, electrical conductivity (ECe), sodium adsorption ratio (SAR), and exchangeable sodium percentage (ESP) were greater than the criteria of salinesodic soil, and soils collected from sites 1.5-2 km away from the coastline were not substantially affected by the intrusion and spray of seawater. Halotolerant bacteria showed similar trends, whereas non-tolerant bacteria and enzymatic activities had opposite trends. Significant positive correlations were found between EC, exchangeable Na+, and pH with SAR and ESP. In contrast, ECe, SAR, ESP, and exchangeable Na+ exhibited significant negative correlations with bacterial populations and enzyme activities. The results of this study indicate that the soil chemical variables related with salinity-sodicity are significantly related with the sampling distance from the coastline and are the key stress factors, which greatly affect microbial and biochemical properties.

Regulation of Ethylene Biosynthesis Under Salt Stress in Red Pepper (Capsicum annuum L.) by 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Deaminase-producing Halotolerant Bacteria

Siddikee, Md. Ashaduzzaman,Chauhan, Puneet S.,Sa, Tongmin Springer-Verlag 2012 Journal of plant growth regulation Vol.31 No.2

Halotolerant bacteria with ACC deaminase activity alleviate salt stress effect in canola seed germination

Md. Ashaduzzaman Siddikee,Subbiah Sundaram,MURUGESANCHANDRASEKARAN,김기윤,셀바,사동민 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2

Amelioration of salt stress effect on canola seed germination was investigated using 1-aminocyclopropane- 1-carboxylic acid (ACC) deaminase-producing plant growth promoting halotolerant bacteria. NaCl at 120 mM concentration reduced canola seed germination by 50 %. The inoculation of ACC deaminase-producing halotolerant Brevibacterium epidermidis RS15 and Bacillus aryabhattai RS341 at 120 mM NaCl significantly increased the seed germination with decreased seed ACC content. Notably, the hydrolytic enzymes activities like amylase, invertase, and protease also increased due to inoculation of RS15 and RS341 compared to uninoculated salt stress imposed germinating canola seeds. Ethylene emission of salt stress exposed eight-day-old canola seedlings was reduced by 35.4 and 41.1 % compared to uninoculated salt stressed control due to respective inoculation of RS341 and RS15. The amelioration of salt stress inhibitory effect on the canola seed germination was attributed to the inoculation of ACC deaminase-producing halotolerant bacteria modulating ethylene emission and inducing hydrolytic enzymes.

Halotolerant bacteria with ACC deaminase activity alleviate salt stress effect in canola seed germination

Siddikee, Md. Ashaduzzaman,Sundaram, Subbiah,Chandrasekaran, Murugesan,Kim, Kiyoon,Selvakumar, Gopal,Sa, Tongmin The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2

Amelioration of salt stress effect on canola seed germination was investigated using 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing plant growth promoting halotolerant bacteria. NaCl at 120 mM concentration reduced canola seed germination by 50 %. The inoculation of ACC deaminase-producing halotolerant Brevibacterium epidermidis RS15 and Bacillus aryabhattai RS341 at 120 mM NaCl significantly increased the seed germination with decreased seed ACC content. Notably, the hydrolytic enzymes activities like amylase, invertase, and protease also increased due to inoculation of RS15 and RS341 compared to uninoculated salt stress imposed germinating canola seeds. Ethylene emission of salt stress exposed eight-day-old canola seedlings was reduced by 35.4 and 41.1 % compared to uninoculated salt stressed control due to respective inoculation of RS341 and RS15. The amelioration of salt stress inhibitory effect on the canola seed germination was attributed to the inoculation of ACC deaminase-producing halotolerant bacteria modulating ethylene emission and inducing hydrolytic enzymes.

Halotolerant Plant Growth Promoting Bacteria Mediated Salinity Stress Amelioration in Plants

Shin, Wansik,Siddikee, Md. Ashaduzzaman,Joe, Manoharan Melvin,Benson, Abitha,Kim, Kiyoon,Selvakumar, Gopal,Kang, Yeongyeong,Jeon, Seonyoung,Samaddar, Sandipan,Chatterjee, Poulami,Walitang, Denver,Chan Korean Society of Soil Science and Fertilizer 2016 한국토양비료학회지 Vol.49 No.4

Soil salinization refers to the buildup of salts in soil to a level toxic to plants. The major factors that contribute to soil salinity are the quality, the amount and the type of irrigation water used. The presented review discusses the different sources and causes of soil salinity. The effect of soil salinity on biological processes of plants is also discussed in detail. This is followed by a debate on the influence of salt on the nutrient uptake and growth of plants. Salinity decreases the soil osmotic potential and hinders water uptake by the plants. Soil salinity affects the plants K uptake, which plays a critical role in plant metabolism due to the high concentration of soluble sodium ($Na^+$) ions. Visual symptoms that appear in the plants as a result of salinity include stunted plant growth, marginal leaf necrosis and fruit distortions. Different strategies to ameliorate salt stress globally include breeding of salt tolerant cultivars, irrigation to leach excessive salt to improve soil physical and chemical properties. As part of an ecofriendly means to alleviate salt stress and an increasing considerable attention on this area, the review then focuses on the different plant growth promoting bacteria (PGPB) mediated mechanisms with a special emphasis on ACC deaminase producing bacteria. The various strategies adopted by PGPB to alleviate various stresses in plants include the production of different osmolytes, stress related phytohormones and production of molecules related to stress signaling such as bacterial 1-aminocyclopropane-1-carboxylate (ACC) derivatives. The use of PGPB with ACC deaminase producing trait could be effective in promoting plant growth in agricultural areas affected by different stresses including salt stress. Finally, the review ends with a discussion on the various PGPB activities and the potentiality of facultative halophilic/halotolerant PGPB in alleviating salt stress.

Inoculation Effect of Methylobacterium suomienseon Growth of Red Pepper under Different Levels of Organic and Chemical Fertilizers

Min-Kyoung Lee(이민경),Gil-Seung Lee(이길승),Woo-Jong Yim(임우종),In-Soo Hong(홍인수),Pitchai Palaniappan,Md. Ashaduzzaman Siddikee,Hari P. Deka Boruah,Munusamy Madhaiyan,Ki-Sup Ahn(안기섭),Tongmin Sa(사동민) 한국토양비료학회 2009 한국토양비료학회지 Vol.42 No.4

화학비료와 유기질비료를 시비 수준 및 Methylobacterium suomiense CBMB120의 접종이 고추 생육에 미치는 영향을 확인하기 위하여 유기질비료와 화학비료의 시비량을 권장시용수준의 100%, 75% 및 50%로 처리하고 Methylobacterium suomiense CBMB120을 접종 한 후 정식 후 19, 36, 및 166일에 작물의 높이를 측정하였으며 이식 후 166일에 건물량 측정하였다. 균주를 접종한 처리구의 식물체 높이는 정식 후 19, 36일에 각각 14.17%, 10.03% 증가하였다. 건물 중 역시 10.30%, 6.84% 증가하여 유의성 있는 차이를 나타내었다. 또한 균주 접종은 유기질 비료 100% 시용구는 36일 후 고도의 유의성(p<0.01)을 갖는 차이를 보였으며 화학 비료 100% 시용구는 19일 후 고도의 유의성(p<0.01)을 갖는 차이를 나타내었다. 화학비료 100% 시용구에 균주를 접종하지 않은 처리 구와 화학비료 75% 시용구에 균주를 접종한 처리구의 생육을 분석한 결과 유의성 있는 차이가 없는 것으로 나타났다. 이는 향후 다양한 처리량 및 처리방법의 연구를 통하여 Methylobacterium suomiense CBMB120 균주의 접종이 화학비료를 일정 부분 감비 할 수 있다는 가능성을 시사한다. Use of plant growth promoting symbiotic and non-symbiotic free-living beneficial bacteria as external source of nitrogen is a major research concern for sustainable crop production in the 21st century. In view of this, an experiment was conducted under controlled conditions to determine the effects of inoculation with Methylobacterium suomiense CBMB120, a plant growth promoting (PGP) root and shoot colonizer on red pepper, for the purpose of reducing external chemical nitrogen fertilization. Amendments with organic fertilizer and chemical fertilizer in the form of NPK were made at dosages of 50%, 75% and 100%, at 425 and 115 kg/ha-1 measurements. The soil type used was loam, with a pH of 5.13. The growth responses were measured as plant height at 19, 36 and 166 days after transplantation and final biomass production after 166 days. It was found that inoculation with M. suomiense CBMB120 promotes plant height increase during the active growth phase at 19 and 36 days by 14.17% and 10.03%, respectively. Thereafter, the bacteria inoculated plantlets showed canopy size increment. A highly significant inoculation effect on plant height at p<0.01 level was found for 100% level of organic matter and chemical amendment in red pepper plantlets after 36 days and 19 days from transplantation. Furthermore, there was a significantly higher (10.30% and 6.84%) dry biomass accumulation in M. suomiense CBMB120 inoculated plants compared to un-inoculated ones. A 25% reduction in the application of chemical nitrogen can be inferred with inoculation of M. suomiense CBMB120 at with comparable results to that of 100% chemical fertilization alone. Enumeration of total bacteria in rhizosphere soil confirms that the introduced bacteria can multiply along ther hizosphere soil. Large scale field study may lead to the development of M. suomiense CBMB120 as an efficient biofertilizer.