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- 저자 : ( Shailendra Raghuwanshi ) (검색결과 3 건)
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Swati Misra,Shailendra Raghuwanshi,Pritesh Gupta,R. K. Saxena 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.2
The experiment described in this paper synthesizes xylitol acylated products from fermentative derived xylitol and acid anhydrides of various chain lengths in the presence of Tetrahydrofuran (THF) and acetonitrile using immobilized Pseudomonas aeruginosa (PL) lipase as a biocatalyst (97% residual activity up to five cycles) at 37°C, 200 rpm. This study examines a number of different acid anhydrides for their highly selective and efficient lipase-catalyzed acylation of primary hydroxyl groups in xylitol. Of those studied, the best results are obtained with butanoic anhydride, 80.12% after 4 h in acetonitrile followed by vinyl acetate, which results in 77.79% conversion after 8 h of incubation in THF as analyzed through high performance liquid chromatography (HPLC).
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Vinod Kumar,Firdaus Jahan,Shailendra Raghuwanshi,Richi V. Mahajan,Rajendra Kumar Saxena 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4
A novel and simple method was developed for the preparation of magnetic Fe3O4 nanoparticles by chemical co-precipitation method and subsequent coating with 3-aminopropyltrimethoxysilane (APTMS) through silanization process. Magnetic Fe3O4-chitosan particles were prepared by the suspension cross-linking and covalent technique to be used in the application of magnetic carrier technology. The synthesized immobilization supports were characterized by scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and X-ray diffraction (XRD). Using glutaraldehyde as the coupling agent, the lipase from R. oryzae was successfully immobilized onto the functionalized magnetic Fe3O4-chitosan beads. The results showed that 86.60% of R. oryzae lipase was bound on the synthesized immobilization support. This immobilized lipase was successfully used for the esterification of phenolic acid which resulted in esterification of phenolic acid in isooctane solvent reaction system for 8 consecutive cycles (totally 384 h), 72.6% of its initial activity was retained,indicating a high stability in pharmaceutical and industrial applications.
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( Senthil Kumar Subramani ),( Rohit Sharma ),( Arjun Yadav ),( Shailendra Raghuwanshi ),( Gbks Prasad ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Liver disease is one of the most common and occurs asymptomatically until disease affects a significant part of the organ. Probiotics are live microorganisms promoted with claims that they provide health benefits when consumed adequate amount, health benefits including antidiabetic, anti-inflammatory and antioxidant properties. This study aimed to determine the effects of probiotic supplementation on liver function and oxidative stress biomarkers in Type II Diabetes mellitus (T2DM) patients. Liver disease is one of the most common and occurs asymptomatically until disease affects a significant part of the organ. Probiotics are live microorganisms promoted with claims that they provide health benefits when consumed adequate amount, health benefits including antidiabetic, anti-inflammatory and antioxidant properties. This study aimed to determine the effects of probiotic supplementation on liver function and oxidative stress biomarkers in Type II Diabetes mellitus (T2DM) patients. Methods: In a randomized double-blind placebo-controlled clinical trial, 60 Subjects with aged 30-65 years were selected from CTR, Jiwaji University and assigned into two groups; Subjects in the probiotic group received a daily capsule containing Lactobacillus acidophilus, Bifidobacterium bifidium & Saccharomyces boulardii each 0.5 Bn/g and Lactobacillus plantarum, Bacillus clausii each 0.25 bn/g with 125mg FOS. BD for 12 weeks. (Placebo group only 125mg FOS in a capsule). In the baseline and at the end of the study, physical activity levels, and dietary intakes were assessed. Anthropometric parameters, blood glucouse, Liver function markers malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), and catalase (CAT) activities were measured. Statistical analysis was carried out using a paired t-test and student t- test. Results: There was no significant difference between the two groups for demographic characteristics, anthropometric parameters at the baseline of study. The mean fasting blood glucose levels were reduced by 16.3%.The probiotic supplementation resulted in a significant improvement in bilirubin by 22.3%, 15.9% ALP 17%,10.2% SGOT 27.2, 22.5%, SGPT 17.9, 17.1% GTT 13.9, 11.9% in subjects taken probiotic placebo capsules respectively. It also observed that the significant increase in GSH (P<0.01), SOD (P<0.01), CAT (P<0.01) and decrease in MDA level (P<0.01). Significant difference was observed between-group for these enzymes activities & other parameters at the end of the study. Conclusions: Overall, the results demonstrate that probiotics could improve liver function and oxidative stress factors among T2DM patients.
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