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Madhaiyan, Munusamy,Poonguzhali, Selvaraj,Lee, Jung-Sook,Saravanan, Venkatakrishnan Sivaraj,Lee, Keun-Chul,Santhanakrishnan, Palani Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.7
<P>A methylotrophic nitrogen-fixing bacterial strain, Ah-143<SUP>T</SUP>, isolated from the rhizosphere soil of field-grown groundnut was analysed by a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis combined with <I>rpoB</I> gene sequence analysis allocated strain Ah-143<SUP>T</SUP> to the family <I>Enterobacteriaceae</I>, with <I>Enterobacter radicincitans</I> and <I>Enterobacter cowanii</I> as the closest relatives. The strain is Gram-stain-negative, non-spore-forming, aerobic and motile, having straight rod-shaped cells with a DNA G<I>+</I>C content of approximately 53.2 mol%. The strain utilizes methanol as a carbon source and the <I>mxaF</I> gene was closely related to the <I>mxaF</I> gene of members of the genus <I>Methylobacterium</I>. The fatty acid profile consisted of C16 : 0, C17 : 0 cyclo, C18 : 1<I>ω</I>7<I>c</I>, summed feature 2 (iso-C16 : 1 I and/or C14 : 0 3-OH) and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1<I>ω</I>7<I>c</I>) as the major components. DNA-DNA relatedness of strain Ah-143<SUP>T</SUP> with its close relatives was less than 20 %. On the basis of the phylogenetic analyses, DNA-DNA hybridization data, and unique physiological and biochemical characteristics, it is proposed that the strain represents a novel species of the genus <I>Enterobacter</I> and should be named <I>Enterobacter arachidis</I> sp. nov. The type strain is Ah-143<SUP>T</SUP> (=NCIMB 14469<SUP>T</SUP> =KCTC 22375<SUP>T</SUP>).</P>
Madhaiyan, Munusamy,Poonguzhali, Selvaraj,Senthilkumar, Murugaiyan,Pragatheswari, Dhandapani,Lee, Jung-Sook,Lee, Keun-Chul International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.2
<P>Three novel bacterial strains, designated Vu-144<SUP>T</SUP>, Vu-7 and Vu-35, were isolated on minimal medium from rhizosphere soil of field-grown cowpea and subjected to a taxonomic study using a polyphasic approach. Cells of the strains were Gram-stain-negative, non-motile, non-spore-forming, coccoid rods, and formed non-pigmented colonies. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Vu-144<SUP>T</SUP> was affiliated with an uncultivated lineage of the phylum <I>Bacteroidetes</I>. Its closest phylogenetic neighbour was the recently described species <I>Niastella populi</I>, a member of the family <I>Chitinophagaceae</I>, with just 90.7 % sequence similarity to the type strain. The only isoprenoid quinone detected was menaquinone 7 (MK-7). The fatty acid profiles showed large amounts of iso-C<SUB>15 : 0</SUB>, iso-C<SUB>17 : 0</SUB> 3-OH and iso-C<SUB>15 : 1</SUB> G and minor amounts of summed feature 3 (C<SUB>16 : 1</SUB>ω7<I>c</I> and/or iso-C<SUB>15 : 0</SUB> 2-OH), C<SUB>16 : 0</SUB> and other fatty acids, allowing the differentiation of the strains from other genera. The G+C content of the genomic DNA of the three strains ranged from 43.1 to 44.3 mol%. In addition to phosphatidylethanolamine, the major polar lipids were three unidentified aminophospholipids (APL1–APL3), two unidentified phospholipids (PL1, PL2) and three unidentified lipids (UL1–UL3). Biochemical test patterns also differed from those of <I>Niastella populi</I> and members of other genera. All three isolates showed plant-growth-promoting properties, e.g. the ability to produce indole-3-acetic acid and NH<SUB>3</SUB> and to solubilize phosphate, utilized 1-aminocyclopropane 1-carboxylate (ACC) as a sole source of nitrogen and possessed the ACC deaminase enzyme. The novel isolates readily colonized roots and stimulated growth of tomato and cowpea under glasshouse conditions. Inoculated plants showed a 45–60 % increase in dry matter weight with respect to uninoculated controls. On the basis of the evidence from our polyphasic study, isolate Vu-144<SUP>T</SUP> represents a novel genus and species in the family <I>Chitinophagaceae</I>, for which the name <I>Arachidicoccus rhizosphaerae</I> gen. nov., sp. nov. is proposed. The type strain of <I>Arachidicoccus rhizosphaerae</I> is Vu-144<SUP>T</SUP> ( = KCTC 22378<SUP>T</SUP> = NCIMB 14473<SUP>T</SUP>).</P>
Madhaiyan, Munusamy,Kim, Byung-Yong,Poonguzhali, Selvaraj,Kwon, Soon-Wo,Song, Myung-Hee,Ryu, Jeoung-Hyun,Go, Seung-Joo,Koo, Bon-Sung,Sa, Tong-Min Microbiology Society 2007 International journal of systematic and evolutiona Vol.57 No.2
<P>A pink-pigmented, facultatively methylotrophic bacterium, strain CBMB20T, isolated from stem tissues of rice, was analysed by a polyphasic approach. Strain CBMB20T utilized 1-aminocyclopropane 1-carboxylate (ACC) as a nitrogen source and produced ACC deaminase. It was related phylogenetically to members of the genus Methylobacterium. 16S rRNA gene sequence analysis indicated that strain CBMB20T was most closely related to Methylobacterium fujisawaense, Methylobacterium radiotolerans and Methylobacterium mesophilicum; however, DNA-DNA hybridization values were less than 70 % with the type strains of these species. The DNA G+C content of strain CBMB20T was 70.6 mol%. The study presents a detailed phenotypic characterization of strain CBMB20T that allows its differentiation from other Methylobacterium species. In addition, strain CBMB20T is the only known member of the genus Methylobacterium to be described from the phyllosphere of rice. Based on the data presented, strain CBMB20T represents a novel species in the genus Methylobacterium, for which the name Methylobacterium oryzae sp. nov. is proposed, with strain CBMB20T (=DSM 18207T=LMG 23582T=KACC 11585T) as the type strain.</P>