http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Li Hui Zou ),( Hai Jian Zhao ),( Dag Uang Wang ),( Meng Wang ),( Chuan Bao Zhang ),( Fei Xiao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.7
Aspartate aminotransferase (AST; E.C. 2.6.1.1), a vitamin B6-dependent enzyme, preferentially promotes the mutual transformation of aspartate and α-ketoglutarate to oxaloacetate and glutamate. It plays a key role in amino acid metabolism and has been widely recommended as a biomarker of liver and heart damage. Our study aimed to evaluate the extensive preparation of AST and its application in quality control in clinical laboratories. We describe a scheme to express and purify the 6His-AST fusion protein. An optimized sequence coding AST was synthesized and transformed into Escherichia coli BL21 (DE3) strain for protein expression. Ideally, the fusion protein has a volumetric productivity achieving 900 mg/l cultures. After affinity chromatography, the enzyme activity of purified AST reached 150,000 U/L. Commutability assessment between the engineered AST and standard AST from Roche suggested that the engineered AST was the better candidate for the reference material. Moreover, the AST showed high stability during long-term storage at -20ºC. In conclusion, the highly soluble 6His-tagged AST can become a convenient tool for supplying a much better and cheaper standard or reference material for the clinical laboratory.
Li, Xiao-Li,Zhou, Fa-Ming,Shangguan, Shou-Qin,Zou, Wen-Qin,Deng, Yan-Qing,Chen, Tao,Chen, Guang-Hui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8
Objective: To explore the value of computed tomography (CT) in the differential diagnosis of glioma stroke and simple cerebral hemorrhage. Materials and Methods: A total of 45 patients with glioma stroke and stroke as the initial symptom in our hospital from Jun., 2009 to Oct., 2013 were selected along with 50 individuals with simple cerebral hemorrhage in the same period randomly collected as a control group. The CT results in both groups were analyzed and compared. Results: In the observation group, there were 25 patients with astrocytoma (55.6%), 11 with oligodendroglioma (24.4%), 8 with ependymoma (17.2%) and 1 with glioblastoma multiforma (GBM, 2.22%). Additionally, the major CT manifestation was coexistence of hemorrhage and tumor signs. By comparison, it could be found that the proportions of patients respectively with peripheral edema and space-occupying effect in the observation group were significantly higher than in the control group (P<0.01). Conclusions: Application of CT examination combined with medical history in patients has very important clinical value in the differential diagnosis of glioma stroke and simple cerebral hemorrhage.
Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage
Li-ming Wu,Rui Guo,Lin Hui,Yong-gang Ye,Jing-mei Xiang,Chun-yun Wan,Miao Zou,Rui Ma,Xiao-zhuan Sun,Shi-jin Yang,Ding-zong Guo 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.4
Chronic enteritis can produce an excess of reactive oxygenspecies resulting in cellular damage. Stanniocalcin-1(STC-1)reportedly possesses anti-oxidative activity, the aim of thisstudy was to define more clearly the direct contribution ofSTC-1 to anti-oxidative stress in cattle. In this study, primaryintestinal epithelial cells (IECs) were exposed to hydrogenperoxide (H2O2) for different time intervals to mimic chronicenteritis-induced cellular damage. Prior to treatment with 200μM H2O2, the cells were transfected with a recombinantplasmid for 48 h to over-express STC-1. Acridine orange/ethidium bromide (AO/EB) double staining and trypan blueexclusion assays were then performed to measure cell viabilityand apoptosis of the cells, respectively. The expression of STC-1and apoptosis-related proteins in the cells was monitored byreal-time PCR and Western blotting. The results indicated thatboth STC-1 mRNA and protein expression levels positivelycorrelated with the duration of H2O2 treatment. H2O2 damagedthe bovine IECs in a time-dependent manner, and this effectwas attenuated by STC-1 over-expression. Furthermore, overexpressionof STC-1 up-regulated Bcl-2 protein expression andslightly down-regulated caspase-3 production in the damagedcells. Findings from this study suggested that STC-1 plays aprotective role in intestinal cells through an antioxidant mechanism.
Physical Layer Security for Two-Way Relay NOMA Systems with Energy Harvesting
Hui Li,Yaping Chen,Borong Zou 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.6
Due to the wide application of fifth generation communication, wireless sensor networks have become an indispensable part in our daily life. In this paper, we analyze physical layer security for two-way relay with energy harvesting (EH), where power splitter is considered at relay. And two kinds of combined methods, i.e., selection combining (SC) and maximum ratio combining (MRC) schemes, are employed at eavesdropper. What’s more, the closed-form expressions for security performance are derived. For comparison purposes, this security behaviors for orthogonal multiple access (OMA) networks are also investigated. To gain deeper insights, the end-to-end throughput and approximate derivations of secrecy outage probability (SOP) under the high signal-to-noise ratio (SNR) regime are studied. Practical Monte-Carlo simulative results verify the numerical analysis and indicate that: i) The secure performance of SC scheme is superior to MRC scheme because of being applied on eavesdropper; ii) The secure behaviors can be affected by various parameters like power allocation coefficients, transmission rate, etc; iii) In the low and medium SNR region, the security and channel capacity are higher for cooperative non-orthogonal multiple access (NOMA) systems in contrast with OMA systems; iv) The systematic throughput can be improved by changing the energy conversion efficiency and power splitting factor. The purpose of this study is to provide theoretical direction and design of secure communication.
( Li Cui ),( Bin Zhang ),( Shuting Zou ),( Jing Liu ),( Pingrong Wang ),( Hui Li ),( Zhenhai Zhang ) 한국미생물생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.2
In this study we sought to elucidate the therapeutic effects of fenchone on constipation-predominant irritable bowel syndrome (IBS-C) and the underlying mechanisms. An IBS-C model was established in rats by administration of ice water by gavage for 14 days. Fenchone increased the reduced body weight, number of fecal pellets, fecal moisture, and intestinal transit rate, and decreased the enhanced visceral hypersensitivity in the rat model of IBS-C. In addition, fenchone increased the serum content of excitatory neurotransmitters and decreased the serum content of inhibitory neurotransmitters in the IBS-C rat model. Meanwhile, western blot and immunofluorescence experiments indicated that fenchone increased the expressions of SCF and c-Kit. Furthermore, compared with the IBS-C model group, fenchone increased the relative abundance of Lactobacillus, Blautia, Allobaculum, Subdoligranulum, and Ruminococcaceae_UCG-008, and reduced the relative abundance of Bacteroides, Enterococcus, Alistipes, and Escherichia-Shigella on the genus level. Overall, fenchone ameliorates IBS-C via modulation of the SCF/c-Kit pathway and gut microbiota, and could therefore serve as a novel drug candidate against IBS-C.
Roles of mTOR and p-mTOR in Gastrointestinal Stromal Tumors
Li, Jun-Chuan,Zhu, Hong-Yu,Chen, Ting-Xuan,Zou, Lan-Ying,Wang, Xiao-Yan,Zhao, Hui-Chuan,Xu, Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
Objective: This study aimed to examine the relationship between expression of mammal target of rapamycin (mTOR) and phosphorylation of mTOR (p-mTOR) protein in the PI3K/Akt/mTOR signaling pathways in gastrointestinal stromal tumors and relatiuonships with clinical factors. Methods: Immunohistochemistry was used to detect the expression of the associated proteins mTOR, p-mTOR, and phosphorylation of the tumor suppressor genes PTEN, P27, VEGF, and EGFR in 40 cases of gastrointestinal stromal tumors, with division into a very low and low risk group as well as a moderate and high risk group. Results: The positive rate of mTOR and p-mTOR was significantly increased in the moderate and high risk group compared with the very low and low risk group. The difference was statistically significant (P<0.05). When grouped according to size, the positive mTOR expression rate exhibited a statistical difference (P<0.05), which was significantly increased in the group of tumors larger than 5 cm. The difference in the positive mTOR and p-mTOR expression rate exhibit no statistical significance among the PTEN, P27, VEGF, and EGFR expression subgroups (P>0.05). Conclusion: The different expressions of mTOR and p-mTOR in the signal transduction pathway of gastrointestinal stromal tumor in the different degree-of-risk groups suggested that the mTOR and p-mTOR of the signal transduction pathway serve an important function in the occurrence and development of gastrointestinal stromal tumors.
Low-Temperature Synthesis of Near-Monodisperse Globular MoS2 Nanoparticles with Sulphur Powders
Hui Wang,Xuequan Li,Kai Yan,유계성,Weixing Song,Ting Shen,Dechun Zou 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.8
Nanoparticles (NPs) with high uniformity have been extensively investigated for their excellent chemical stability. Near-monodisperse globular MoS2 NPs were prepared with sulphur powders (SPs) as a sulphur source by a one-pot polyol-mediated process without surfactants, transfer agents and toxic agents at 170–190 ℃. The as-processed SPs greatly affected the formation of the MoS2 NPs after low-activity sulphur (S8)n was reassembled from common SPs (S8). The average size of MoS2 NPs can be reduced remarkably from 100–200 nm to 50 nm by introducing low amounts of MnCl2. A preliminary four-step growth mechanism based on the aggregation-coalescence model was also proposed. This green and simple method may be an alternative to the common hot-injection and heating-up methods for the preparation of monodisperse NPs, particularly transition metal dichalogenides.
QI-HUI SHEN,YAN LIU,XI YU,XIAO-YANG LIU,MING-QIANG ZOU,JIN-FENG LI,JIAN-GUANG ZHOU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.6
II?VI Semiconductor nanocrystals (NCs) with tunable visible emission, such as CdS, CdSe and CdTe, were synthesized in aqueous solution using thiols as capping molecules. Hydrazine was found to promote the growth of NCs through a special mechanism. In only a few hours, the synthesis process was completed at room temperature. Under moderate conditions, the capping molecules not only changed the growth rate of NCs simply by varying the concentration, but also altered the spectral properties of NCs. The capping molecules with amino groups were propitious to the growth of CdS NCs, whereas the kinetic growth of CdS NCs was more a®ected by the surface passivation e±ciency of NCs than by steric hindrance in the system. The fastest growth of the CdS NCs was achieved when glutathione was used as a capping molecule, while the emission of CdS and CdSe NCs were shown to remain steady and tunable using the same capping molecule. The growth rate of 3-mercaptopropionic acid-capped CdS and CdSe NCs slowed down signi¯-cantly, while CdTe NCs were obtained with excellent emission properties when capped with the same molecule. Furthermore, our approach will also be useful for the study of the kinetic growth of NCs in aqueous solution.
SSR and SCAR mapping of a multiple-allele male-sterile gene in Chinese cabbage (Brassica rapa L.)
Feng, Hui,Wei, Peng,Piao, Zhong-Yun,Liu, Zhi-Yong,Li, Cheng-Yu,Wang, Yu-Gang,Ji, Rui-Qin,Ji, Shu-Juan,Zou, Ting,Choi, Su-Ryun,Lim, Yong-Pyo Springer-Verlag 2009 TAG. Theoretical and applied genetics. Theoretisch Vol.119 No.2