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한국산 시궁쥐(Rattus norvegicus)의 핵형분석
김상식,손성원,이정훈 慶南大學校 附設 基礎科學硏究所 1997 硏究論文集 Vol.10 No.-
In this study, we analysed the karyotypes of the Korean brown rat (Rattus norvegicus) collected in Kyungnam Masan and Changwon. The diploid number (2n) was 42 and the fundamental number (FN) was 60. In the autosomes, metacentrics consisted of three pairs of the middle form and two pairs of the small from, submetacentries consisted of two pairs of the middle form and a pair of the small form, subtelocentrics consisted of a pair of the large form and a pair of the middle form, and acrocentrics has 10 pairs of large to small form. In sex chromosomes, X-chromosome was subtelocentrics of the middle form, and Y-chromosome was acrocentrics of the small form.
Sex change scale and pattern of Tegillarca granosa (Bivalvia: Arcidae) in 2011-2012
Mi Ae Jeon,Hyejin Kim,Kayeon Ku,Jung Sick Lee 한국발생생물학회 2015 한국발생생물학회 학술발표대회 Vol.2015 No.9
The sex of bivalves is classified into gonochorism and hermaphroditism, and hermaphroditism is further divided into simultaneous, and sequential. Simultaneous hermaphroditism is the simultaneous release of eggs and sperm by one organism during the same season. Sequential hermaphrodites are either male or female for one or several annual cycles (Heller, 1993; Gosling 2004; Collin, 2013). T. granosa is a sequential hermaphroditic bivalve undergoing sex change (Lee et al., 2014). However, definitive conclusion on whether the scale and pattern of sex change of T. granosa is always consistent could not be made. Therefore, the objective of this study was to reconfirm sex change in T. granosa and consider the scale and pattern of sex change compared to the results of Lee et al. (2014). The total number of T. granosa used for sex change identification was 777 with a shell length (SL) between 25.1-35.0 (30.9±2.13) mm. For Tegillarca granosa, the scale of sex change during 2006-2007 was reported to be 15.1% (Lee et al., 2014). In this study, the overall scale of sex change in T. granosa was 37.6% during 2011-2012, which was approximately 2.5 times higher than that reported by Lee et al. (2014). In addition, the difference between the sex change ratio from females to males and that from males to females was 15.3% during the period of 2011-2012, which was similar to the finding of 15.0% during 2006-2007 (Lee et al., 2014). The sex change ratio of female→male : male→female was 1 : 3.42 during 2006-2007 reported by Lee et al. (2014). It was 1 : 1.57 during 2011-2012 in this study.
Intersexuality of Crassostrea gigas and Ruditapes philippinarum in Southern Coastal Waters of Korea
Jung Sick Lee,Yeon Gyu Lee,Seung Wan Kang,Ji Seon Park,Dong Geun Lee,Mi Ae Jeon,Sun Mi Ju 환경독성보건학회 2010 환경독성보건학회지 Vol.25 No.4
Objectives : The aim of this study was to verify the intersexuality of Crassostrea gigas and Ruditapes philippinarum in southern coastal waters of Korea. Methods : Specimens of Crassostrea gigas (n=363) were collected from six areas of Tongyeong, Geoje and Yeosu. Ruditapes philippinarum (n=221) was collected from five areas of Yeosu. The sex ratio and intersexuality were determined after observing gonad preparations. Results : The rate of intersexuality in Crassostrea gigas was 16.25%, with females (24.79%; n=30/121) exhibiting a higher rate than males (11.98%; n=29/242). The rate of intersexuality in Ruditapes philippinarum was 24.43%, with females (37.76%; n=37/98) exhibiting a higher rate than males (13.82%; n=17/123). Conclusions : The results of this study suggest that intersexuality is induced by aquatic pollutants such as endocrine disrupting chemicals (EDCs).
A Rubrofusarin Gentiobioside Isomer from Roasted Cassia tora
Lee, Hee-Jung,Jung, Jee-Hyung,Kang, Sam-Sick,Choi, Jae-Sue The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.5
From the roasted seeds of Cassia tora L., a new naphthopyrone glycoside was isolated and characterized as 10-[${\beta}$-D-glucopyranosyl-$(1{\rightarrow}6)-O-{\beta}-D-$glucopyranosyl)oxy]-5-hydrox y-8-methoxy-2-methyl-4H-naphtho[1, 2-b]pyran-4-one(isorubrofusarin gentiobioside). Along with isorubrofusarin gentiobioside, alaternin and adenosine were isolated and identified.
Lee Jung-Sick,Ju Sun-Mi,Park Ji-Seon,Jin Young-Guk,Shin Yun-Kyung,Park Jung-Jun 한국발생생물학회 2010 발생과 생식 Vol.14 No.1
This study attempted to verify the possibility of using germ cell aspiration (GCA) method as a non-fatal technique in studying the life-history of equilateral venus, Gomphina veneriformis (Veneridae) and granular ark, Tegillarca granosa (Arcidae). Using twenty-six gauge 1/2" (12.7mm) needle, GCA was carried out in equilateral venus through external ligament. In granular ark, GCA was performed by preventing closure of the shells by inserting a tongue depressor between the shells while still open. The success rate of sex identification using the GCA method was 95.6% for the equilateral venus (n=650/680) and 94.3% for the granular ark (n=707/750). Mortality of equilateral venus, which spent 33 days under wild conditions, was 13.8% (n=90/650) while the mortality of granular ark, which spent 390 days under wild conditions, was 2.4% (n=17/707). Although we believe that GCA does not appear to cause death in equilateral venus or granular ark, the success rate in employing of this methodology may differ depending on the level of proficiency of the researcher and reproductive stage of the bivalve. This study concludes that GCA is a convenient non-fatal methodology, which can be employed to identify sex and investigate gonadal maturity in Gomphina veneriformis and Tegillarca granosa.
Lee Jung-Sick,Ku Ka-Yeon,Kim Hye-Jin,Park Ji-Seon,Park Jung-Jun,Shin Yun-Kyung,Jeon Mi-Ae 한국발생생물학회 2012 발생과 생식 Vol.16 No.3
This study attempts to propose the possibility of the sex reversal in Tegillarca granosa and Ruditapes philippinarum by confirming the changes in the sex ratio with the shell length (SL) in the same population level. For analysis of sex ratio with SL, 1500 individuals of T. granosa (SL 10.1-45.0 mm) and 712 individuals of R. philippinarum (SL 15.1-70.0 mm) were used. Sex was analyzed histologically. The average sex ratios (F:M) of T. granosa and R. philippinarum were 1:1.22 and 1:0.96, respectively. However, sex ratio was found to differ when the clams were divided into groups according to SL in 5.0 mm intervals. Both species displayed the tendency of increase in the proportion of female with increase in SL. In this study, changes in the sex ratio in accordance with the growth of T. granosa and R. philippinarum are determined to be indirect evidence that signifies their sex reversal.
Intersexuality of Crassostrea gigas and Ruditapes philippinarum in Southern Coastal Waters of Korea
Lee, Jung-Sick,Lee, Yeon-Gyu,Kang, Seung-Wan,Park, Ji-Seon,Lee, Dong-Geun,Jeon, Mi-Ae,Ju, Sun-Mi The Korean Society of Environmental Toxicology 2010 환경독성보건학회지 Vol.25 No.4
Objectives : The aim of this study was to verify the intersexuality of Crassostrea gigas and Ruditapes philippinarum in southern coastal waters of Korea. Methods : Specimens of Crassostrea gigas (n=363) were collected from six areas of Tongyeong, Geoje and Yeosu. Ruditapes philippinarum (n=221) was collected from five areas of Yeosu. The sex ratio and intersexuality were determined after observing gonad preparations. Results : The rate of intersexuality in Crassostrea gigas was 16.25%, with females (24.79%; n=30/121) exhibiting a higher rate than males (11.98%; n=29/242). The rate of intersexuality in Ruditapes philippinarum was 24.43%, with females (37.76%; n=37/98) exhibiting a higher rate than males (13.82%; n=17/123). Conclusions : The results of this study suggest that intersexuality is induced by aquatic pollutants such as endocrine disrupting chemicals (EDCs).
불볼락 ( Sebastes thompsoni ) 의 생식주기
(Jung Sick Lee),(Cheul Min An),(Sung Hoi Huh) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.1
Sexual maturation and reproductive cycle of the goldeye rockfish, Sebastes thompsoni were investigated under photomicroscopy. Samples were collected monthly in the coastal water of Samcheonpo(34°55′N), Korea from November 1995 to October 1996. The ovary consists of several ovarian lamellae originated from ovarian outer membrane. Oogonia which are originated from the inner surface of the ovarian lamella protrude to the ovarian cavity in oocyte stage, and they are suspended by the egg stalk. The testis is seminiferous tubule type in internal structure. Seminiferous tubule consists of many testicular cysts which contain numerous germ cells in same developmental stage. Biological minimum size of female and male were 19.5㎝ and 21.5㎝ in total length, respectively. Gonadosomatic index(GSI) of female was the highest(9.56) in March and the lowest(0.15) in August. GSI of male was the highest(0.25) in February and the lowest(0.04) in July. Reproductive cycle was classified into the following successive stages: in female, growing(October and November), maturation(December-February), gestation(March), parturition and recovery(April-June) and resting(July-September), and in male, growing(September-November), maturation December and January), ripe and copulation (February and March) and degeneration and resting(April-August).
Lee, Joung-Hun,Kim, Youn-Sub,Kim, Young-Sick,Kim, Sung-Eun,Sung, Yun-Hee,Kim, Bo-Kyun,Lee, Jin-Woo,Ham, Dae-Hyun,Lee, Hye-Jung,Kim, Chang-Ju Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.7 No.5
Shenqi-wan, Oriental herbal medicine formulation, has traditionally been used for the treatment of delayed mental and physical development in children, complications of diabetes, and glomerulonephritis. In the present study, we investigated the protective effect of the aqueous extract of Shenqi-wan and its fractions against N-methyl-D-aspartate (NMDA)-induced exitotoxicity in rat hippocampal CA1 neurons. Fractions were elucidated at 0 - 10 min, 11 - 20 min, and 21 - 30 min by using gravity column chromatography method. In the present results, treatment with NMDA on cultured hippocampal slices induced neuronal death in the hippocampal CA1 region. Pretreatment with the Shenqi-wan did not exerted protective effect, however its fractions suppressed NMDA-induced neuronal damage. The fraction elucidated at 11 - 20 min showed the most potent protective effect. These results revealed that effective substances of the Shenqi-wan against NMDA-induced excitotoxicity may exist mainly in the fraction elucidated at 11 - 20 min.