Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

과학 교육에서 STS 수업모형의 적용효과에 대한 메타 분석

정미진,윤기순,권덕기 경북대학교 과학교육연구소 2008 科學敎育硏究誌 Vol.32 No.2

이 연구의 목적은 STS 과학교육에 대한 국내 연구의 동향을 분석하고 메타분석을 통해 STS 수업모형의 적용효과를 평가하는데 있다. 연구대상 논문은 1991년부터 2006년까지 출판된 석사학위 논문 104편과 과학교육 저널에 출판된 논문 78편을 포함한 총 180편이었다. STS 수업 모형의 효과 평가에 총 56편이 선정되었다. STS 수업 모형 적용 효과의 평균효과크기는 0.40이었다. 이 결과는 STS 수업은 학생들의 과학에 대한 태도, 과학 학업 성취도, 탐구능력, 환경에 대한 태도 및 환경에 대한 지식을 향상시키는데 전통적인 수업보다 긍정적인 효과가 있었음을 의미한다. STS 수업은 환경에 대한 태도의 증진에 가장 큰 평균효과 크기를 나타내었다. 그러므로, STS 수업에서 가장 좋은 효과를 얻기 위해서는 이러한 결과들을 반영하는 것이 필요할 것이다. 향후 STS 교육에 대한 전반적인 평가를 하기 위해서는 STS 수업 모형의 효과와 과학-기술-사회의 상호관계에 대한 교사와 학생들의 이해에 대한 연구 결과를 종합하는 것이 필요하다. The purposes of this study were to analyze the domestic research trend of the STS Education and to evaluate the application effects of STS teaching model by using Meta-analysis. The selected research articles were 180 articles including 104 of the master’s theses and 76 of science education journal articles published from 1991 to 2006. For the evaluation of the effects of STS teaching and learning model, 56 articles were selected finally. The mean effect size of the application effects of STS teaching and learning was 0.40. The result indicated that STS teaching had more positive effects than the traditional teaching on enhancing student's attitude for science, academic achievement in science, inquiry ability, attitude for environment and knowledge for environment. Especially, it had shown the most positive effects on improvement in the attitude for environment. Therefore, it might be better to reflect these results for the best effect of STS teaching. To assess STS education on the whole, it is necessary to synthesize the effects of STS teaching and learning model and the results of the study on teachers’ and students’ understanding about the interrelation of science, technology and society.

스포츠 공학의 연구 동향

윤양진,정미라,이훈식,서국웅,박승범 釜山大學校 附設 體育科學硏究所 1998 體育科學硏究所 論文集 Vol.14 No.-

Sports technology encompasses a wide range of disciplines from biomechanics to engineering. As a consequence, researchers working in sports technology find that their work falls neither in the field of traditional sports nor in mainstram engineering. Also Sports Engineering is an international journal publishing original papers on the application of engineering and science to sport. The journal intends to fill the niche area which lies between classical engineering and sports science and aims to bride the gap between the analysis of the equipment and of the athlete. Area of interest include the mechanics and dynamics of sport, the analysis of movement, instrument equipment design, surface interaction, materials and modeling. These topics may be applied to technology in almost any sports. The Sports Engineering will be of particular interest to Engineering, Physics, Mathematics and Sports Science Departments and The International Sport Engineering Association(ISEA) will act forum where research, industry and the sports sector can exchange knowledge and innovative ideas.

특발성 자발성 신장 파열 1예

윤재철,김원,조규종,홍정석,이미우,장성은,오세현,임경수 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Primary aldosteronism is a syndrome characterized by hypertension, hypokalemia, suppressed plasma renin activity, and elevated serum aldosterone levels. Conn first described it 1955 in association with adrenal cortical adenoma(Conn's syndrome). Today, it can be divided into at least six distinctive sub-groups. The diagnosis of primary aldosteronism is usually suspected when the patient presents with poorly controlled hypertension with hypokalemia. The main issues in the evaluation of primary aldosteronism are to differentiate an adenoma from hyperplasia and to localize the adenoma. Basic hormonal studies and computed tomographic(CT) scanning can be used effectively for that differentiation. An adenoma is one of a few potentially curable forms of hypertension, and it is best treated by removing the adrenal tumor. We experienced a case of a typical adrenal adenoma. The patient was a 37-year-old male who had experienced in both his legs over a period of several days a weakness due to hypokalemia. He was diagnosed using basic hormonal studies and adrenal CT scanning. After, he received laparoscopic adrenalectomy and was discharged with improved condition.

테니스 포핸드시 밀어치기와 때려치기 타법의 운동학적 분석

윤양진,정미라,서국웅,이훈식,황명숙,강영택,윤길환 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

This study consists in clarifying the meaning of various angles and velocity, and two types of the stroke of a pushing shot and hitting shot through the three-dimensional image analyses among the factors of exercise. To attain the purpose of such studies, the result of experiment and analysis for each of three athletes of boys and girls in high school and three persons in general came to the conclusion as follows; 1. The last motion of a group of A having a command of a follow shot showed greatly, compared with two groups of B and C, and this showed that a group of A took a rectilinear motion greatly to gain power and two group of B and C took a rotary motion greatly to gain speed. 2. A group of A having a command of a pushing shot move body weight forward from behind and give the first consideration to a rectilinear motion, and two groups of B and C having a command of the stroke of hitting shot used the turning of the shoulder and the waist and gave much weight to a rotary motion than a rectilinear one. 3. The position of the hitting point of a group of A having a command of a pushing shot turned out to be the waist, and that of the hitting point of two groups of B and C, the chest, and namely this showed that the position of the hitting point was decided according to the form of the stroke and pictures of various kinds. 4. In impacting, the speed decreased, for the power was controled to keep an accurate timing of stroke just before impacting

인지발달이론에 기초한 유아 도덕교육의 이론적 기초연구

윤미혜,김정준 기전여자대학 1992 論文集 Vol.12 No.-

The object of this study is to give the theoritical bases of the approach method of appropriate moral education of cognitive development for Children. For this, the theorities of Piaget, Kohlberg and Rest was to be reviewed, analyzed and compared in detail. As the results of this study constructural method of moral education are; 1. The use of strategy based on cognitive development. 2. Democratization of classroom society. 3. Discussion of moral dilemmas.

실리콘 중합체 - 메타아크릴산 중합체의 상호침투 가교고분자의 합성 및 성질에 관한 연구

윤구식,정미라,박유미 東亞大學校附設基礎科學硏究所 1998 基礎科學硏究論文集 Vol.15 No.2

소수성 중합체인 실리콘과 수용성 중합체인 폴리메타아크릴산(PMAA)을 이용하여 친수성과 소수성을 함께 가진 양친성의 상호침투 중합체(IPN)를 합성하였다. DSC를 이용하여 합성한 중합체(IPN)의 열적 성질을 조사해 본 결과 IPN은 PMAA의 영역과 실리콘 중합체 영역의 두 상으로 분리되어 있었다. 증류수 속에서 중합체의 수팽윤도는 PMAA의 함량이 증가할수록 증가하였으며, pH에 따른 수팽윤도는 pH 4.0 < 증류수 < pH 7.2 < pH 10.7 순으로 증가하였다. 또한 용해성 파라미터 값을 달리하는 몇 가지 용매에 대한 중합체의 팽윤도를 구해본 결과 IPN내의 PMAA의 함량이 증가할수록 용해성 파라미터 값이 큰 물, 에탄올의 팽윤도 값이 커졌으며, 용해성 파라미터 값이 작은 옥탄의 평윤도는 반대의 현상을 나타내었다. 또한 부탄올의 팽윤도는 두가지 단순중합체에서는 낮았으나 IPN에서는 조금 증가하였다. The interpenetrating polymer networks(IPNs) composed of silicone polymer and poly(methacrylic acid)(PMAA) was prepared and characterized by FTIR and DSC. The IPN has the two separated phases which consist of silicone domains and PMAA domains. The sorption of water increased with increasing PMAA contents in the IPN, and at pH10.7 water was absorbed most and the second was at pH7.2. As increasing of the contents of PMAA in the IPN, the sorption of polar solvent was increased and that of nonpolar was decreased.

비만 개선 효과를 지닌 탄수화물 및 지방 흡수 억제 기능성 식이조성물 개발

윤유식,최선미,홍순복,홍정미,김정원,이홍석,홍성길 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

In a previous study, we developed a new food additive as an egg yolk antibody (IgY) against carbohydrate digestion enzymes for the regulation of blood glucose level and weight control. The IgY delayed and decreased the increment of blood glucose level after administration of sucrose in human being by 30% in 20∼30 min. We also developed a lipase inhibitor as a water extract of two kinds of herb, Platycodon grandiflorum and Solanum melongena. Twenty three volunteers were subjected to the intake of the egg yolk IgY plus the herbal extracts for 50 days. In average, the treated subjects appeared to lose 1.96 ㎏ of body weight and 3.4 ㎏ of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of egg yolk IgY and natural herbal extract improve the obesity by the decrement of body weight and body fat mass.

임팩트시 테니스 라켓 재질의 차이에 따른 근전도 분석

윤양진,유국종,서국웅,정미라,강영택,이훈식,서국은 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this paper is to select 7 male athletes and female athletes of high school, and to compare their active potential differences of upper arm muscles according to characteristic of a racket. the results obtained are as follows: 1. According to the accumulative distribution rate of racket material, graphite and wood material show better efficiency in case of brachio radialis muscle of male athletes, but the racket material shows no constant efficiency in brachii radialis muscle of female athletes. In case of flexor carpi radialis muscle, wood material provides better efficiency only for male athletes. In case of triceps brachii muscle, wood material gives better efficiency to males athletes and steel material gives better efficiency to females athletes. Especially in case of biceps brachii muscle, neither male nor female athletes are influenced by racket materials at impact. 2. In the muscle load rate according to racket material, wood has the highest muscle active potential and graphite has the lowest active potential. Therefore, graphite is thoght to be better in the efficiency of muscle load rate.