Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

蒼耳子에 대한 면역글로블린E 생성억제 효과

宋昊埈,河顯秀,金台現,辛民敎 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

We studies inhibitation effect of IgE release in the human B cell line(U266B1) and in the serum and spleen cell of mouse that has been immunized by antigen in order to find antiallergic effect of Fructus Xanthii (FX). We have some findings as below. 1. IgE release in serum was inhibited dose dependently in case of administering FX orally in the mouse immunized. 2. IgE release in serum was inhibited dose dependently in case of injecting FX into abdomen in the mouse immunized. 3. When we take LPS+IL-4 way in the mouse spleen cell, IgE release was less than LPS way. 4. When we take LPS+IL-4 way in the human B cell line (U266B1), IgE release was less than LPS way.

필기체 숫자인식을 위한 평균장 아닐링모델

송치화,김호준,이원돈 충남대학교 기초과학연구소 1997 忠南科學硏究誌 Vol.24 No.2

This paper proposes a neural network approach to solve the hand-written digit recognition problem. The proposed approach is based on the graph matching, a form of elastic matching, and a mean field annealing algorithm. In general, a conventional recognition model uses a preprocessor to remove meaningless variations and to capture meaningful features. But this approach can recognize digits without any preprocessing such as thinning, scaling and segmentation.

韓國産 노박덩굴科 藥用植物에 關한 文獻硏究

宋昊埈,金裕鎭 대한본초학회 1996 大韓本草學會誌 Vol.11 No.1

This study aims to survey through literature the origin of Celastraceae (belonging to the class of wild spimach) which grow in nature or are cultivated in Korea. The results of survey can be summarized as follows; 1. The total 40species of wild spinach surveyed consisted of 7 Celastrus, 32 Euonymus, 1 Tripterygium; Euonymus occupies 80% of these species. 2. The wild spinaches of the Korean origin were found to be 3genuses, 40species, among which the herb species occupied about 56% 3. The herb species out of the wild spinaches were 7 ones according to the nomenclature of Korean oriental medicine. They were 24 root species, 20 leaf species. 3 seed species, 20 cortex species, 9 fruit species, 6 exocarpium species, I flora species. Thus it was noticed that radix was the main kind. 4. In view of their traits, they were 10 antife brile species, 5 neutral in nature species and 12 hot-natured species. 5. In view of their dfficacy and following for duplicate nomenclature, they can be classified into 6 heat-clearing and detoxifying herbs, 6 insecticides, 16 expel wind alleviate itching herbs, 8 promote blood circulation hemostatic herbs, I gastrotomic herbs 9 promote diuresis to reduce edema herbs and 1 invigratic herbs. 6. The toxic herbs were lekondung, kijunyu, etc. However, expect lekondung, their toxity was weak and they accounted of 48% of the total herb plants. As discussed above the herb plants account for 56% of the total wild sponaches. Among them the root species are ranked atop. It is conceived that these herb plants should be surveyed more for their clinical efficacy.

세신뿌리가 아나필락시에 미치는 영향

宋昊埈 대한본초학회 1997 大韓本草學會誌 Vol.12 No.2

This study was investigated the inhibitory activity of RADIX ASARI (RA) on type I immediate hypersensitivity of the anaphylactic type. RA inhibited compound 48/80-induced anaphylaxis 100% with a dose of 0.5 g/㎏ body weigh at 1 h before or 5 and 10 min after injection of compound 48/80. RA also exhibited the dose-dependent inhibitory effect on compound 48/80-induced, Substance P-induced and anti-DNP IgE-induced histamine release from rat peritoneal mast cells. These results suggest the possibility that RA induced inhibition of type I allergic reaction is regulated by histamine.

메틸水銀의 心筋毒性에 대한 苦蔘의 影響

송호준,하대호,유교상,박승택,이강창,서부일 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

Objectives : To evaluate the toxic effect of methylmercuric chloride(MMC) and the protective effect of Sophorae Radix(SR) in cultured mouse myocardial cells, cytotoxicity was measured by MTT assay Methods : Cultured myocardial cells were incubated for 72 hours in the media containing 5~40 ㎛ concentrations of MMC. And also, the protective effect of SR was determined by sulforhodamine B(SRB) for protein sythesis and radioactive assay for DNA sythesis in these cultures. Results : Cell viability was significantly decreased in a dose-dependent manner after cultured myocardial cells were exposed to 20 ㎛ MMC for 72 hours. In the neuroprotective effect of SR on MMC-induced cytotoxicity, SR blocked the MMC-induced myotoxocity in these cultures. Conclusion : It suggests that MMC is toxic against cultured mouse myocardial cells and SR is effective in blocking the meurotoxicity induced by MMC.

건설 PMIS 실태와 건설 CALS 연계 및 확산방안에 관한 연구

송병관,박영호,백준홍 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

This study has aimed for wide spreading of CALS in Construction Industry. The PMIS produce original information of construction project linked to CALS. So, this study have investigated present state of PMIS in construction industry, and reviewed the interface to construction CALS. And, key issues of adopting PMIS & CALS to whole construction and its related industry have been found. Additionally, it has also found improvement issues of construction system and political supports for PMIS adoption.

Constituents Analysis of Amino Acid and Antioxidative Activity from Cultivated Callus and Rhizome in Rhodiola sachalinensis

Song,Won-Seob,Chi,Hyung-Joon,Rim,Yo-Sup,Yoon,Jae-Ho 한국자원식물학회 2002 Plant Resources Vol.5 No.1

The material of Rhodiola sachatinensis collected from an alpine region of the west-northern China. For analysing the effect, 1 used Rhodiola sachatinensis s rhizome and cultivated callus. In EtOAc, BuOH, H₂0separation the plant showed strong antioxidative activity, but not in Hexane. The radical scavenging effect of EtOAc(RC50, 35(g), BuOH(RC50, 43(g), H₂0(RC50, 50(g) fraction and MeOH extract(RC50, 50(g) of the Rhodiola sachatinensis was comparable to that of synthetic antioxidant BHA(RC50, 14(g) and α-Tocopherol(RC50, 12(g). Total amino acid concentration of plant of In nature condition were 18,009ppm, and major components were arginine, glutamic acid, aspartic acid and valine. The ratio of essential/total amino acid on plant of In nature condition was 46.93%. Total amino acid concentration of callus of In vitro condition were 32,435ppm, and major components were valine, histidine, lysine and leucine. The ratio of essential/total amino acid on callus of In vitro condition was 56.07%. was 56.07%.

崔孤雲 詩의 詩想 構造 : 그 몇가지 特色

송준호 연세대학교 국어국문학과 1977 연세어문학 Vol.9-10 No.-

失意의 美學 : 未發表 漢文小說 丁生傳 考

宋寯鎬 연세대학교 국어국문학과 1974 연세어문학 Vol.5 No.-