재발성 류마티즘 환자에서 발작의 유발인자

주유철,이동민,양종태,신병철,임태영,서정균,정기영,최동헌,김원,조영신,고희관 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : Palindromic rheumatism (PR) is known to be triggered without any obviously inciting events in most patients, although, in a few cases, attacks have been noted to relate to another events such as weather change, childbirth, ingestion of certain foods, or over-exercise. The aim of this study was to characterize the triggering factors on palindromic attacks. Materials and Methods : We evaluated sixty-four patients with PR for the presence of triggering factors and the relationship between attacks and triggering factors by interview of patients. We considered that there was an probable association between episodes and PR, if episodes within 24 hours prior to PR had triggered at least 50% of all attacks and a definite association between episodes and PR, if episodes within 24 hours prior to PR had triggered 100% of all attacks. Results : In 15 patients (35.9%), there was a possible association between episodes of PR and initiating events. In 8 patients (12.5%), there was a definite association between episodes of PR and initiating events. The triggering factors were physical over-activity including exercise (19/64: 29.7%), foods, such as rawfish, crab, and chicken, including alcohol (19/96: 19.8%). However, there were no difference between the groups with and without the triggering factor in sex ratio, duration and onset of PR, the positive rate of rheumatoid factor and involved sites. Conclusion : Our observations showed that PR was triggered by physical over-activity more than in previous reports and hypersensitivity to foods was a causative factor in some patients with PR. However, patients with triggering factors had a similiar clinical profile to patients without triggering factors.

균 spheroplast 및 large unilamellar vesicle의 식물 protoplast로의 도입

주충노,강철호,이희봉 연세대학교 자연과학연구소 1987 學術論文集 Vol.19 No.-

A. tumefaciens의 식물 protoplast로의 도입을 확인하는 방법으로 방사성 인산(Na_2H^32PO_4)을 함유한 0.8% nutrient broth에서 증식한 Agrobacterium tumefaciens C58를 사용하여 방사성 spheroplast와 방사성 Ti plasmid encapsulated liposome을 만들어 Danus carota protoplast로의 도입을 시도한 결과 다음과 같은 실험 성적을 얻었다. 1. A. tumefaciens에서 추출한 Ti plasmid를 Reverse Phase Evaporation 법으로 phosphatidyl-choline, stearylamine과 함께 Ti plasmid encapsulated liposome을 제조한 결과 17%의 Ti plasmid가 liposome안으로 편입되었다. 2. 방사성 Ti plasmid encapsulated liposome과 Dacus carota root protoplast를 polyethyleneglycol-Ca^2+법으로 처리한 결과 introduction frequency는 38%이었다. 3. A. tumefaciens C58의 spheroplast와 Dacus carota root protoplast를 polyethyleneglycol Ca^2+ 법으로 처리한 결과 introduction frequency가 47.6%였다. 4. 본 연구에서 얻은 실험결과로 부터 liposome법, spheroplast법 모두 Ti plasmid의 식물 protoplast로의 도입은 용이하게 일어나는 것으로 생각된다. It was attempted in the present study to prepare radioactive Ti plasmid to identify the introduction frequency of Ti plasmid encapsulated liposome into Dacus carota root protoplast. Agrobcterium tumefaciens C58 was cultured in 0.8% nutrient broth containing radioactive phosphoric acid(H_3^32PO_4) and the radioactive Ti plasmid wad isolated. Radioactive Ti plasmid was encapsulated into phosphatidyl choline-stearylamine liposomes by reverse phase evaporation technique and the encapsulation frequency was found to be about 17%. Radioactive Ti plasmid encapsulated liposome and Dacus carota root protoplast was treated by polyethyleneglycol Ca^2+ procedure. The introduction frequency was 38%. The spheroplast of A. tumefaciens C38 was prepared from the bacteria cultured in 0.8% nutrient broth containing radioactive phosphoric acid using lysozyme. As much as 47% of the spheroplast were found to be introduced into Dacus carota protoplast.

중첩 방전을 이용한 CO₂레이저의 동작 특성 : 다중펄스 중첩을 이용한 long 펄스형 CO₂레이저의 펄스 형상 제어 Pulse shape control of pulse CO₂laser using multiple-pulse superposition

정현주,김근용,정용호,정종한,송건주,김희제 부산대학교 생산기술연구소 2001 生産技術硏究所論文集 Vol.60 No.-

본 연구에서는 PIC 원 칩 마이크로 프로세서 기술을 접목시켜, 저전압 측에서 2단 또는 3단의 회로를 능동적으로 중첩시킴으로써, 다양한 펄스폭을 가지는 고전압의 펄스형상을 만들었다. 이렇게 만들어진 다양한 중첩펄스를 현재 산업용 및 의료용으로 많이 사용되고 있는 CO2 레이저에 적용하여 각 단의 스위치의 트리거 지연시간에 따른 레이저 빔의 출력 특성을 고찰하였다. 그 결과 다양한 펄스폭을 가지는 레이저 빔을 얻을수가 있었고 특히, 3단 중첩회로에서 스위칭 지연 시간을 변화시킴에 따라 얻어진 레이저 펄스폭은 최소 약 4ms였고 최대 약 10ms였다. In this study, a variable pulse length, high duty cycle Pulse Forming Network(PFN) is constructed by time sequence. The power supply for this experiment consists of three switching circuits. Each switching circuit uses 2 mesh PFN, a charging circuit, a HV leakage transformer, and bridge rectifier on each transformer secondary. The PFN elements operate at low voltage and drive the primary of HV leakage transformer. The secondary of the transformer has a full-wave rectifier, which passes the pulse energy to the load in a continuous sequence. We investigated laser pulse width of pulsed CO2 laser as various delay time among three switching circuits. As a result, we can obtain various laser pulse width from about 4 to 10ms.

CORBA 기반 SNMP 게이트웨이

정영준,송화선,서희정,김주영 강원대학교 정보통신연구소 1999 정보통신논문지 Vol.3 No.-

As telecommunicaton networks becomes more complex and intelligence, it is essential to develop an integrated network management system between heterogeneous telecommunication networks. To fulfil such demands, one solution is CORBA, which provides a distributed platform, in this paper, we present the design and implementation of a gateway between CORBA and SNMP, called as an Internet management protocol. The proposed scheme allows a CORBA managerto access the SNMP MIB without special transformation of SNMP managed objects and only but using SNMP API which the gateway provides. Also since managed objects are represented in CORBA IDL, a new SMI can be updated only by recompiling the interface. Consequently this gateway approach gives a great of advantages in a point that any changes of managed object implementation on managed agents MIBs are not required.

Glucose-Acetate 資化細菌에 의한 L-Glutamic acid生成

鄭熙鍾,鄭大柱,鄭址炘 全南大學校 農漁村開發硏究所 1986 農業科學技術硏究 Vol.21 No.-

A glucose-acetate assimilating bacterium was investigated for the production of L-glutamic acid. The bacterium was isolated and identified as Brevibacterium saccharolyticum sp, from the various soil samples, which utilized glucose-acetate as a carbon source for the production of L-glutamic acid. Thirty one strains including this isolate produced more than 10g/ℓof L- glutamic acid. The maximum yield of L glutamic acid at the optimal conditions, 2% glucose, 2% sodium acetate, 1% ammonium acetate, 3~4㎍/ℓbiotin, pH 7.5, 30℃, was 28g/ℓ.

Pseudomonas putida SM25로부터 Protocatechuate 3,4-dioxygenase를 암호하는 pcaHG 유전자의 분자적 클로닝 및 염기서열 분석

정영희,민경희,권주영 숙명여자대학교 자연과학연구소 1998 자연과학논문집 Vol.- No.9

Pseudomonas putida SM25로부터 protocatechuate 3,4-dioxygenase 를 암호하는 pcaHG 유전자를 클로닝하기 위하여 이미 알려져 있는 primer를 제작한 후 P. putida SM25의 염색체 DNA를 주형으로 하여 PCR product인 약 1.6 kb DNA 절편을 얻었다. 이 DNA 절편을 SalI과 EcoRV로 절단한 약 1 kb DNA 절편만을 probe로 사용하여, PstI으로 절단한 염색체 DNA 전편과 Southern blot을 실시한 결과, 4.4 kb의 PstⅠ DNA 절편을 얻었다. 이 절편을 pUC19 vector에 삽입하여. 재조합 플라스미드 pYJ27을 얻었으며, 이 재조합 플라스미드의 SphI, EcoRV, Pstl,SalI, Aval 등을 사용하여 제한효소 지도를 작성하였다. Insert DNA를 서브 클로닝 하여 pcaHG 유전자를 포함하는 DNA 절편의 염기서열을 결정한 결과, pYJ27에는 1336bp 크기인 두개의 open reading frames(ORFs)가 존재하였으며 protocatechuate 3,4-dioxygenase에 해당되는 437개의 아미노산을 암호하였다. 이미 보고된 protocatechuate 3,4-dioxygenases의 DNA 염기서열을 비교한 결과, Pseudomonas putida ATCC 23975와는 94% 정도의 비교적 높은 homology를 보였다. 또한 Rhodococcus나 Acinetobacter에서는 각각 63%, 55%의 homology를 보여 주었다. Protocatechuate 3,4-dioxygenase catalyzes the conversion of protocatechuate to β-carboxymuconate through the cleavage of the aromatic ring. The genes encoding subunits of the protocatechuate 3,4-dioxygenase were designated pcaH and pcaG, respectively. The locations of the genes for the α and β subunits of protocatechuate 3,4-dioxygenase on a 4.4 kb PstI fragment cloned from the Pseudomonas putida SM25 chromosome were determined. A 1.6-kb DNA fragment from a 4.4kb recombinant DNA was cloned into pUC19 vector, pYJ27. Recombinant plasmid pYJ27 was mapped with various restriction enzymes, SalI, EcoRV, PstI, SphI, and AvaI. According to bidirectional subcloning and method of dideoxynucleotide chain termination, we determined the nucleotide sequences of the recombinant DNA fragment pYJ27 containing pcaHG gene. The deduced amino acid sequences of pcaG and pcaH were consisted 200 and 237 amino acid, respectively. The pcaG and pcaH showed about 94% homology to that of P. putida ATCC 23975 in the nucleotide sequences.

Agrobacterium tumefaciens의 Ti plasmid 재조합에 관한 연구

주충노,이희봉,김영명 연세대학교 자연과학연구소 1987 學術論文集 Vol.19 No.-

Crown gall의 병원체로 알려진 Agrobacterium tumefaciens가 함유하고 있는 Tumor inducing(Ti) plasmid가 식물 hormone과 opine의 합성원인이라는 것이 밝혀지고 식물 세포의 crown gall에는 Ti plasmid가 존재하며 Ti plasmid의 일부가 식물세포의 핵 genome에 삽입되어 형질 변화가 일어남이 보고 되었고 Ti plasmid의 식물체로의 gene도입 vector로서의 연구가 최근 활발히 진행되고 있으며 현재 PLGV vector와 PMON vector 등이 개발되고 있으나 조작이 복잡하고 아직은 초보적 단계인 것으로 알려져 있다. 본 연구에서는 원하는 식물 gene을 식물체에 도입하는 유용한 vector개발의 초보단계로서 Ti plasmid의 T-DNA의 일부인 nos gene을 분리하여 pBR322와의 재조합을 기도한 것이다. pBR 322를 포함하는 E. coli로 부터 Horwitz(1979)의 방법으로 pBR 322를 분리하였고 A. tumefaciens C58에서 Kado(1979)의 방법으로 Ti plasmid를 분리한 후 Ti plasmid 내의 nos gene을 분리하였다. 분리된 pBR 322를 Hind Ⅲ로 절단한 후 CIP로 처리하고 T_4 DNA ligase를 이용하여 Hind Ⅲ로 처리한 nos gene fragment와 재조합하였다. 이와같이 하여 얻은 recombinant DNA로 인한 E. coli HB101의 형질 변화를 Mandel과 Higa의 방법에 따라 조사한 결과 pBR 322의 tetracyclin gene 사이에 nos gene이 삽입된 것으로 확인되었다. 즉 ampicillin 배지에서는 92개의 colony가 형성되었는데 그중 59개는 tetracyclin 배지에서 colony를 형성하지 못하였다. 이러한 tetracyclin 감수성이면서 ampicillin 저항성인 colony를 배양하여 얻은 recombinant plasmid를 전기이동법으로 분리한 결과 예상대로 크기가 7.5kb 정도였고 Hind Ⅲ로 절단하였을 때 2개의 band가 확인되었다. 본 연구에서 얻은 nos gene-pBR 322 hybrid plasmid는 원하는 식물 gene을 이것에 삽입하여 A. tumefaciens 내에서 homologous recombination을 행한 후 식물체에 감염시켜 원하는 gene의 발현여부를 검출하는데 이용될 것으로 기대된다. It is mow realized that the tumor-inducing(Ti) plasmid in Agrobacterium tumefaciens is responsible for the induction of crown gall tumors in dicotyledonous plants and it has been demonstrated that a segment of the Ti plasmid, so called T-DNA, is stably integrated into and expressed in the genome of transformed plant cells. Recently, the use of A. tumefaciens Ti plasmid as a vector to introduce a foreign gene has been intensively studied and several vectors such as PLGV vector and PMON vector has been reported. However, their practical use is still at a primitive stage. It was attempted in the present study, therefore, to conduct the recombination of nos gene of T-DNA from A. tumefaciens Ti plasmid and E. coli pBR 322 as a vector it introduce foreign plant gene into dicotyledon plant cells. pBR 322 was isolated from E. coli containing pBR 322 accdrding to Horowitz(1979) and nos gene of T-DNA in Ti plasmid from A. tumefaciens C58 was obtained according to Kado(1979). The isolated pBR 322 was cut using Hind Ⅲ followed by the addition of CIP·pBR 322 fragments and Hind Ⅲ treated nos gene fragments were than recombined using T_4 DNA ligase and the E. coli cells were then transformed by recombinant DNA according to Mandel and Higa(1970). It was confirmed that nos gene was inserted into tetracyclin gene of pBR 322 by the finding that when the transformed cells were incubated in the ampicillin medium, 92 colonies were formed, in which 59 were tetracyclin sensitive. The above tetracyclin sensitive but ampicillin resistant cells were then cultured and the recombinant pBR-nos gene was isolated and subjected to electrophoresis. It was found that size of the recombinant DNA was approximately 7.5 Kb as expected and two bands were appeared on electrophoretogram when the recombinant DNA was treated with Hind Ⅲ. It might be possible to insert the foreign plant genes to the pBR-nos gene hybrid plasmid obtained in the present study and the resultant DNA could be then homolgously recombined with Ti plasmid of A. tumefaciens and the final recombined Ti plasmid could be used for the introduction of the foreign plant genes into dicotyledon plant cells.

전국 10개 종합병원 중환자실 환자의 직장내 Vancomycin-resistant Enterococci(VRE) 보균율, 보균의 위험요인 및 유전적 다양성 조사

정희진,송준영,엄중식,김우주,최선주,최정현,이혁,정문현,이광미,신동현,손보라,최종성,우흥정,권정아,이경원,최강원,박승철 대한감염학회 2002 감염 Vol.34 No.5

뇌사환자의 갑상선 기능, 전신대사 및 중증도에 관한 연구

이영주,정금희,왕희정,문봉기,한연희,이영석 아주대학교 의과학연구소 1999 아주의학 Vol.4 No.1

Background and Objectives : Brain death may lead to hormonal depletion, metabolic derangement and multiple organ dysfunction. We have carried out present study to examine the effects of brain death on the thyroid function, metabolic indices, and the severity scoring systems. Methods : 13 adults patients admitted for organ donation or brain death evaluation were examined after brain death was confirmed. Thyroid hormones measured were .thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and free thyroxine (FT4). The metabolic indices measured were arterial ketone body ratio (AKBR), lactic acid (LA), and base defiat (BD). as for reference to the severity scoring systems, APACHE Ⅲ and multiple organ failure score (MOFS) were assessed on the day of brain death confirmation. Arterial blood was drawn for all measurements. Results : As for the thyroid function, there were significant decreases in T3 (40.48±20.96 ng/dL) and T4 (3.47±2.15 ㎍/dL), but no significant change in FT4 (0.75±0.31 ng/dL) and TSH (1.12±1.37 uIU/mL) compared to the normal range. Significant decreases in AKBR (0.39±0.31) and BD (-9.46±5.83 m㏖/L), and significant increase in LA (2.57±2.46 m㏖/L) In metabolic indices, were shown, as for severity scoring systems, APACHE Ⅲ score (101.54±19.41) and MOF score (9.11±2.57) indicated a high mortality. There were significant correlation between thyroid hormones (r=0.565 -0.781) but no correlation among other indices. Also, significant inverse correlations were shown between base deficit and lactic acid (r=-0.660), APACHE Ⅲ score (r=-0.726) and MOF score (r=-0.604). The highest correlation was observed between APACHE Ⅲ score and MOF score (r=0.851). Conclusions : As for the thyroid function, significant decrease in T3 and T4, and almost normal range of FT4 and TSH imply the euthyroid sick syndrome. Abnormal finding of the metabolic parameters indicates an inhibition of the aerobic metabolic rate of the body as a whole. And the severity scoring parameters are compatible with high mortality.

신합성 물질인 Protein kinase A 억제제, H-87의 MDR1 유전자 발현의 조절

박주인,김선희,정병선 부산대학교 유전공학연구소 1992 분자생물학 연구보 Vol.8 No.-

In this study, we examined the effect of H-87, specific inhibitor of protein kinase A, on in vitro sensitivities of mouse leukemia cell line P388 resistant to several antitumor agents. H-87 significantly potentiated the cytotoxic effects of ADM, VCR and VBL on P388 cells resistant to these antitumor agents. Thus we examined the mechanism of overcoming multidrug resistance by H-87. In northern blot analysis, H-87-treated resistant P388 cell lines showed less MDR1 gene expression than untreated control cells. As a result of CAT assay, the expression of MDRI promoter was inhibited by H-87 in a concentration-dependent manner. To compare the effect of H-87 with that of staurosporine(PKC inhibitor), we examined the effect of staurosporine on in vitro sensitivities of mouse leukemia cell line resistant to several antitumor agents. The expression of MDR1 gene in multidrug resistant P388 cells was decreased by staurosporine. The activity of MDR1 promoter was increased by TPA(PKC activator) and forskolin(adenylate cyclase activator). Therefore, we speculate that PKA and PKC may play important roles in the regulation of MDRI gene expression.