토양으로부터 分離한 Bacteriophage의 특성

박현균,김도경,박경진,전홍성,박종필,김종승,김성준 조선대학교 부설 유전생물공학연구소 1992 유전생물공학연구지 Vol.2 No.-

환경 미생물 분야와 산업미생물 분야 및 분자생물학 분야에서 주목을 받고 있고, 많은 연구가 된 새로운 E. coli의 bacteriophage를 토양으로 부터 분리해서, 그 물리적인 특성과 핵산을 밝혀내, phage상호간의 관련성을 검토하여 보았다. 그 결과를 요약하면 다음과 같다. 1. 토양으로 부터 분리한 phage는 숙주균에 따라 SJ-1, SJ-2, SJ-3, SJ-4, SJ-5, SJ-6, SJ-7이라 명명하였다. SJ phage들은 높은 숙주특이성을 나타내 phage SJ-2, SJ一7은 단일종 숙주균에만 감염이 되었고, SJ-1, SJ-3, SJ-4, SJ-5, SJ-6은 다중독성을 나타내었다. 2. 7종류의 SJ-phage는 본 실험조건하에서는 모두 virulent phage임이 밝혀졌다. 모든 용균반의 형태는 중앙이 투명한 둥근 모양이었으며 주변에 무리가 있는 것과 없는것 두가지 형태를 나타냈다. 용균반의 크기는 1-5mm이었으며, SJ-5phage의 용균반이 5mm로서 가장 컸고 SJ-7 phage가 가장 적었다. 숙주균은 OD_600 0.3-0.5상태에서 가장 감염되기가 좋았다. 그리고 상층배지의 한천 농도와 양은 0.5%와 3ml이 가장 좋은 조건이었다. 3. 일단 증식 실험의 결과를 보면 SJ-phage는 잠복기가 15-50분이었고 평균 phage방출수는 190-250이었다. SJ-6과 SJ-7이 잠복기가 15분으로 가장 짧았고 SJ-4가 50분으로 가장 길었다. 평균 방출수는 SJ-3과 SJ-5가 250정도로 많았으며 SJ-1과 SJ-2는 약 190정도였다. 4. SJ-phage는 PD buffer에서 안정하였고, magnesium ion 5×10^-3M의 첨가는 phage에게 큰 안정성을 주었다. SJ-phage는 pH6과 pH9사이에서 안정하였으며 그 pH는 숙주균의 최적 pH였다. SJ-phage의 온도 불활성화는 40 ℃이상에서 불활성화되기 시작하여 60 ℃이상에서는 완전히 불활성화 되었다. 자외선 조사에 의한 불활성화에서 SJ-phage는 120초 이후에 완전히 불활성화 되었다. All phages used in this experiment were isolated from soil of the surburbs of Chun-Nam, and they designated as phage SJ-1, SJ-2, SJ-3, SJ-4, SJ-5, SJ-6, SJ-7. The hosts were JM105, JM109, K802, LE392, MC1000, MV1184 and PSM CC, respectively. These phages were virulent under the experimental conditions. They produced plaques with dear and round without halo. The size of plaques was 1-5mm in diameter. SJ-phages were stable in 5×10^-3M Mg^2+. Thermostability experiments indicated that SJ-phages were stable at 37℃. Optimal pH of the phages were 6-9. Exposure of the phages to U.V. for 120 seconds resulted in complete inactivation. In one-step growth experiments, the latent period at 37 ℃ was about 15-50 min and the average burst see was 190-250. Agarose gel electrophoresis of the phage DNA showed single band of high molecular weight from SJ-1 to SJ-7 phages

엔로플록사신 액제의 닭 호흡기 및 소화기 감염증에 대한 예방 및 치료효과

윤효인,박병권,조준형,정상희,박경환,장범수,김종춘,황윤환,임종환,이내경,박승춘 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

We evaluated the efficacy of the liquid form of enrofloxacin against poultry respiratory and digestive infections, the major problems in the poultry industry, from diverse diagnosis viewpoints. Results obtained through this study were as follows: 1. The liquid form of enrofloxacin showed good acceptability to poultry owing to no abhorrence to the drug by chickens, which indicated its suitability for mixing in drinking water. 2. The application of the enrofloxacin formulation improved the productivity of the chicken farm to a great extent by lowering the death toll of chicken raised in the farm. 3. The use of the drug shortened the days to the market, and increased the body weight at the time of marketing. 4. Preventive and therapeutic effects of the liquid enrofloxacin were observed higher than ampicillin, a major antibiotic used in poultry farming. From above results, the liquid form of enrofloxacin would find its use for the prevention and treatment of digestive and respiratory infections, let alone the growth promotion and feed efficiency, especially in the chicken farming practice.

엔로플록사신 액제의 닭유래 병원세균에 대한 시험관내 항균효과 시험

윤효인,박병권,조준형,정상희,박경환,장범수,김종춘,황윤환,임종환,이내경,박승춘 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

In this study the antimicrobial spectrum and sensitivity of a liquid formulation of enrofloxacin against the major six pathogenic bacteria isolated from the diseased poultry in Korea were evaluated in comparison with ciprfloxacin (CFX), gentamicin (GM) and penicillin (PC). A liquid form of enrofloxacin, a test material and ciprofloxacin showed at least 83% antibacterial activity against all of six test pathogenic microorganisms at the fixed concentration of 10 ㎍/㎖. Gentamicin and penicillin revealed much lower antibacterial activity at the same condition. We evaluated the liquid form of enrofloxacin as having widespread antibacterial spectrum against poultry infections. The liquid form of enrofloxacin showed potent antibacterial activity, with the MIC range being 0.12∼2 ㎍/㎖ against Gram positives, 0.06∼2 ㎍/㎖ against Gram negatives and 0.5∼2 ㎍/㎖ against Mycoplasma spp. These values were equivalent to or somewhat higher than those of ciprofloxacin, but much lower than those of gentamicn and penicillin. This new liquid form of enrofloxacin would find its way in application of mixing with drinking water for the prevention and treatment of diverse infections in chickens.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

보문 : Polyethylene Film포장이 포도 Sheridan의 저장력에 미치는 영향

남상영(Sang Young Nam),김경미(Kyoung Mi Kim),박종천(Jong Chun Park),주선종(Seon Jong Joo),정재현(Jai Hyun Jung) 한국식품저장유통학회(구 한국농산물저장유통학회) 1997 한국식품저장유통학회지 Vol.4 No.1

메조포러스 물질의 세공크기 조절

박천관,김종호,서곤 全南大學校 觸媒硏究所 1998 觸媒硏究 論文集 Vol.20 No.-

The pore sizes of MCM-41 and KIT-1 mesoporous materials could be controlled by the addition of 1,3,5-trimethylbenzene as a complementary chemical or the use of templates with different chain lengths such as ?? and ??. The synthesized mesoporous materials are characterized from X-ray diffraction patterns and N₂adsorption isotherms. With the addition of 1,3,5,-trimethylbenzene, the pore size of synthesized, mesoporous material increased, while the pore size distribution became broad. On the other hand, the mesoporous material synthesized using a template with a larger carbon chain such as ?? showed larger pore size with a narrow distribution.

모유수유 실태에 관한 조사연구 : 경상북도성주군을 중심으로

박천만,김규종,이종렬,김혜진 韓國保健敎育.健康增進學會 2000 보건교육건강증진학회지 Vol.17 No.1

This study to study and analyze the current state of infancy breast-feeding & practice and related factors and to provide basic data for the effective practice of breast-feeding and breast-feeding percentage improvement. For the object, 474 mothers of the infants who were born between Jan. 1, 1998 and Dec. 31, 1998 were selected, and the survey period & method were from Oct. 15, 1999 through Nov. 14, 1999(1 month period) and telephone interview, respectively. To examine by monthly breast-feeding type, the average breast-feeding percentage in 1 month after a child's birth was 42.4%, but after 6 month it decreased to 23.0%. To see breast-feeding percentage by academic background, the breast-feeding of elementary school graduated mothers was 33.3%, which was highest, and it decreased as the academic background of mothers went better(p〈0.01). By employment state the percentage for the mothers who were not employed was higher(24.6%) than that of those who were employed at the time, which was 8.0%(p〈0.001). There was no meaningful difference found in breast-feeding percentage by infant mothers age, delivery place, infant sex, child order in family, weight at the time of birth. To examine breast-feeding percentage by delivery method, natural delivery was 27.0% which was higher that 13.1% of Caesarean section(p〈0.01), and by breast-feeding experience before leaving the hospital, 35.6% of those who have breast-fed were breast-feeding while it was 16.4% for those who never did it while in the hospital(p〈0.001) After conducting logistics multi return analysis having breast-feeding percentage as a variable at the time of 6 months after babys birth, I found that infant mothers employment state, delivery method, and brest-feeding experience before leaving the hospital become meaningful variables. As unemployed mothers(p〈0.05), as chose natural delivery(p〈0.05), as experienced breast-feeding before leaving the hospital(p〈0.001), there was more tendency to do breast-feeding. Summing the above results, I would like to suggest the following to raise breast-feeding percentage. Firstly, systematic education about the importance and advantages of breast-feeding and correct breast-feeding method should be practised for women. Secondly, vacation before and after childbirth for working women should be practised and the support of systems for working womens breast-feeding such as installation of nursery in work place are needed. Thirdly, continuous publicity and education for natural delivery inducement is necessary, and for the ones who chose Caesarean section, recommendation of breast-feeding is also needed. Fourth, for breast-feeding within fastest time after delivery, rooming-in required and legal and systematic support is also needed. Lastly, in the governmental level, publicity for breast-feeding and breast-feeding recommending programs that promotes 10 rules for successful breast-feeding bringing-up, provided by UNISEF and WHO, are to be provided.

Methicillin耐性葡萄球菌에 미치는 抗菌劑 倂用效果

全燾基,朴鍾旭 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2

One hundred and thirty-one strains of staphylococci isolated from various clinical specimens in 1985 were tested for antimicrobial susceptibility to 16 drugs by microdilution broth method, and 24 strains were found to be resistant to methicillin (Mt). Mt-resistant staphylococci (MRS) were in most cases resistant to the other drugs tested except vancomycin (Vc) in high frequency, but most Mt-susceptible staphylococci were susceptible to the drugs except erythromycin (Em), teracycline, gentamicin (Gm), and kanmycin (Km). The combined activity of Mt with the other drugs was tested on MRS by checkerboard and time-killing methods. (FIC) index of 0.05 to 0.5 in 95% of strains. Vc, ampicillin, cephalothin, and penicillin also showed synergistic effects in more than 50% of strains. The combination of oxacillin also showed the synergistic activity with some toehr drugs, such as Em, rifampin, and trimethoprim. Gm was antagonistic with Mt in some strains, and Km was antagonistic with Mt in most strains. Vc was synergistic with Mt, and moxalactam.

휘발성유기화합물(VOCs)의 변이원성 검정

박종천,이용탁,송현철,이우범 國立麗水大學校 環境問題硏究所 2004 環境硏究論文集 Vol.6 No.-

The mutagenicity of six kinds of VOCs(benzene, m.p-xylene, chloroform, toluene, methylethylketone, 1,2-dichloroethane) detected in high concentration and frequency in Kwangyang Bay was investigated. VOCs are water-insoluble and volatile. Therefore, when they are tested by the classical Ames test in the assay of mutagenicity, there may be experimental errors. To overcome this limitation, modified Ames test was used to assay mutagenicity of VOCs. By this modified Ames test, we could obtain much more satisfactory results. Even at extremely low concentration(0.0001㎍/㎖). bezene, m,p-xylene, and methylethylketone showed a weak mutagenicity. When S-9 mixture is added in the assay system, all tested VOCs except methylethylketone showed mutagenicity. When Salmonclla typhimurium TA 98 and Salmonella typhimurium TA 100 are exposed to VOCs of high concentration(100㎍/㎖). the number of colony decreased irrespective of the S-9 mixture treatment. It seem to be that the decrement results from the toxic effect of high concentration of VOCs.

강진만 미생물군집의 계절별 분포

박종천,주현수,이우범 國立 麗水大學校 環境問題硏究所 1999 環境硏究論文集 Vol.2 No.-

The biological and ecological surveys were carried out at six site water region from February to October. 1998, In other to analysis of environmental variation, density population of heterotrophic bacteria, general fulgi and fecal coli were determined on each sample. The result, ecosystem of Gangjin bay were regulated by 2~4 factors for four season. The results showed that distribution of the microbial population was mainly infuluenced by inflow of fresh water, salinity and variation of water temperature. And suggested that environmental factor of Gangjin bay was relatively simple than those other bays.