Modulation of Gene Expressions and Enzyme Activities of Methionine Sulfoxide Reductases by Cold, Aba or High Salt Treatments in Arabidopsis

Oh, Jee-Eun,Hong, Suk-Whan,Lee, Yunsook,Koh, Eun-Ji,Kim, Kideok,Seo, Youngwon,Chung, Namhyun,Jeong, Moonsoo,Chang,Chang, Cheolseong,Lee, Byoungcheon,Kim, Kyoung Heon,Lee, Kwangwon,Lee, Hojoung 생명자원연구 2005 생명자원연구 Vol.13 No.-

영아반 그림책 읽기 활동, 교사의 언어적,비언어적 행동 및 영아의 어휘력 간의 관계

정지은(Jee Eun Jeung),김명순(Myoung Soon Kim) 한국아동학회 2012 아동학회지 Vol.33 No.4

The purpose of this study was to investigate the relationship of toddlers` vocabulary ability with reading activities and teachers` verbal and nonverbal behaviors during book reading. The subjects were 52 teachers and 104 toddlers at age 2. Lee and Kim (2004)`s categories of the Teachers` Verbal Behaviors, the categories of the Nonverbal Behavioral Analysis(Kim. 2005), and the Peabody Picture Vocabulary Test-Revised (Kim, et al.1995) were used. The data was analyzed by t-test, ANOVA, and Correlations. The results indicated that there were significant correlations in the subcategories of reading activities in classrooms, teachers` picture book reading behaviors, and toddler`s vocabulary ability. The toddler`s vocabulary ability increased as the ``interacting time both before and after book reading`` became longer. The toddler`s vocabulary ability increased as ``connecting with previous experience``, ``asking questions for confirmation``, ``acknowledging,`` and ``expanding`` of verbal behavior, as well as the nonverbal behavior, such as ``requesting behavior``, ``emotional contact,`` and ``empathetic behavior.`` In conclusion, teachers` reading behaviors and activities were related to toddlers` vocabulary ability.

국가예방접종전산등록체계 개발을 위한 민간의료 기관 하부구조 및 환경 조사연구

이무식,김은영,이건세,이석구,홍지영,김건엽,김대경,신의철,이연경,기몽서,나백주,김영택 한국모자보건학회 2003 한국모자보건학회지 Vol.7 No.2

키토산 처리가 숙주의 생육 특성에 미치는 영향

김철재,문영은,이지해 숙명여자대학교 2000 生活科學硏究誌 Vol.15 No.-

The effects of chitosan on the germination and growth of mungbean sprouts were investigated on a laboratory scale. Mungbean was separately imbibed in 500 and 1,000 ppm chitosan solutions for 7 hrs followed by sprinkling with distilled water, and the other mungbean was also soaked in 500 ppm chitosan solution followed by sprinkling with 500 ppm chitosan solution, and then kept at 25±1℃ for 5 days. The growth characteristics such as whole length, hypocotyl length and thickness, root length and hair, and sprout yield were maintained and increased in the chitosan-imbibed mungbean. As mungbean grew, the proximate components such as carbohydrate were almost catabolized in the chitosan-imbibed mungbean sprouts during germination. The growth rate of sprouts expressed as whole length per day was comparatively higher in the chitosan-imbibed mungbean. One of the important enzyme during germination, α-amylase, increased its activity until the 4th day and thereafter slowly decreased except 500 ppm chitosan-sprinkled mungbean sprouts showing the lower response in its activity. Consequenctly, when mungbean imbibed in chitosan solution was cultivated, the chemical composition of the resultant sprouts did not change but their growing characteristics and sporut yield were enhanced as compared with others. Therefore, chitosan treatment during the imbibition of mungbean is potential to activate the growth of mungbean but decrease it when sprinkled with chitosan solution.

발아현미가루를 첨가한 머핀 제조조건의 최적화

김철재,이지해,이은혜,문혜진,주나미 숙명여자대학교 건강·생활과학연구소 2006 生活科學硏究誌 Vol.- No.22

In order to develop the functional muffin prepared with different levels of sprouted brown rice flour (SBRF), the mixing conditions of SBRF, butter and water were optimized by response surface methodology(RSM), using central composite design with 3 variables and 5 levels. The result of three-dimensional plot was showed that decrease of SBRF content increased the volume, maximum length, crumb grain, moistness and overall quality of muffin. The overall quality of the functional muffin was maximized under the preparing condition of 31.49 g SBRF(15% of flour used), 85.36 g butter, and 123.86 g water. Moreover, the optimum conditions for preparing muffin were as follow : 29.95 g, 81,24 g, 120.45 g for volume ; 23.67 g, 88.18 g, 118.24 g for maximum length ; 38.44 g, 63.38 g, 117.26 g for flavor ; 29.86 g, 93.04 g, 126.42 g for taste ; and 55.43 g, 94.40 g, 133.61 g for moistness. The range of optimum preparing conditions for the functional muffin in consideration of the physical and sensory characteristics were shown to the 30~40 g as SBRF, 80~90 g as butter, and 120~130 g as water.

프로그램 분위기와 프로그램 관여도에 따른 PPL효과 연구

김정현,이명천,김지은 한국방송광고공사 2008 광고연구 Vol.0 No.78

This study is designed to investigate PPL effects by program mood and program involvement.An experiment was performed to test 4 hypotheses about the main effect of program mood on PPL and interaction effect of program mood and program involvement. 288 subjects were conveniently sampled and randomly assigned to experimental groups. This study adopted 2×2 factorial design by the program mood(positive vs negative) and program involvement(high vs low). The findings were as follows: Firstly, the main effect of program mood was supported. That is, the recall of PPL brand was significantly high when the program mood was negative. And the attitude toward PPL brand was significantly positive when it placed in positive program mood than in negative program mood. Secondly, program involvement and program mood caused interaction effect only to the recall of PPL brand. That is, the brand recall increased when the program mood was negative and the program involvement was low. However, program involvement and program mood did not cause interaction effect to the attitude of PPL brand. 급격한 매체 환경 변화로 촉발된 광고 환경의 변화로 인해 광고효과에 대한 의문이 제기되면서, 광고주 및 실무자들은 전통적인 광고의 약점을 극복하고 설득효과를 제고시킬 수 있는 새로운 방법을 모색하게 되었다. 이에 영화나 드라마 속에 제품을 제시하는 대표적 혼합 메시지라 할 수 있는 PPL(product placement)은 이러한 욕구를 충족시켜 주는 새로운 마케팅 수단으로 활용되고 있다. 본 연구는 국내외적으로 PPL의 활용빈도가 점증하고 있는 현실에 비해 PPL효과를 다룬 실증 연구들이 충분히 축적되어 있지 못한 국내 연구 현실을 고려하여 프로그램 분위기와 프로그램 관여도에 따른 PPL효과를 실험연구를 통해 알아보았다. 구체적으로 본 연구는 PPL효과(브랜드 회상과 브랜드태도)를 종속변인으로, 프로그램 분위기(긍정적/부정적)와 프로그램 관여도(고/저)를 독립변인으로 하는 2×2 요인설계방안을 채택하고 대학생을 대상으로 실험을 실시하였다. 연구 결과 긍정적 분위기보다 부정적 분위기의 프로그램에서 제시된 PPL의 브랜드 회상이 높게 나타났고, 부정적 분위기보다 긍정적 분위기의 프로그램에서 제시된 PPL의 브랜드태도가 높게 나타났다. 따라서 본 연구 결과를 토대로 프로그램 분위기가 PPL에 미치는 효과는 검증되었다고 할 수 있다. 그러나 프로그램 분위기와 프로그램 관여도가 PPL에 대해 상호작용효과를 유발하는가를 검증해본 결과, 브랜드 회상의 경우만 유의하게 나타나 부분적으로만 검증되었다고 할 수 있다.

효과적인 영어학습을 위한 멀티미디어 학습 도구의 설계 및 구현

김지원,이정선,안성은,최황규 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.A

There has been a study about the effective multimedia education using a computer following the appearance of a virtual space. Also, there has been an effort to connect the information & communication technology with education. The popular on-line lecture systems are mostly on English lecture sites. However, they just offer the YOD(Video On Demand) services ignoring students' convenience. To improve these week points, we design and implement the multimedia leaching system focusing on an efficient repeat-effect in order that students can control the Media Player by clicking a sentence on a web page. This paper presents the Editor and Player considering students' interest and the effective learning fruits. So users can easily make multimedia materials and use them to improve their English listening skill.

만 5세 유아를 위한 지역사회 참여 활동의 효과적인 운영에 관한 실행연구

김지은,엄정애 이화여자대학교 교과교육연구소 2014 교과교육학연구 Vol.18 No.1

The purposes of this study were to explore and implement activities for five-year-olds' effective participation in the community and investigate the responses of the children and teachers to the operation of the activities. This study was conducted with a class of twenty-eight, five-year-old children in a private kindergarten in Seoul, Korea. This study was conducted from April until October in 2013, and the community participation activities were analyzed. The conclusions of this study are as follows. Based on a problem analysis of those activities that had been performed previously, the researchers planned and executed the first implementation. In the first implementation, the researchers devised and executed a plan for cultivating a rooftop garden so that the children could utilize the diverse resources of the community. In addition, as a place for education, the study used the community an out-of-classroom venue. Using the assessment results of the first implementation, the second implementation was conducted in a way that involved the public institutions in the community. As a result, the children were helped to understand the necessity for public institutions and organizations which helped the children to solve problems. Also, the Children under study participated in these activities to achieve a common goal, the renaissance of a tree-lined road, and worked together as main agents with community members, such as children, parents and residents. This study suggests a need for five-year-olds to be involved in the community and provides suggestions for the means to achieve this involvement. In addition, it suggests that future studies examine a variety of ways to encourage participation in the Community by using specific human and material resources of the community. 본 연구는 사회과 교육의 한 방법으로 만 5세 유아를 위한 지역사회 참여 활동을 효과적으로 운영하기 위한 방안을 모색하여 실행하고, 실행 과정을 통해 유아와 교사의 반응을 살펴보는데 목적이 있다. 이를 위해 서울특별시에 소재한 사립유치원 만 5세 28명을 연구 참여자로 선정하여 2013년 4월부터 10월까지 활동을 실행하고 수집된 자료를 토대로 그 결과를 분석하였다. 본 연구 결과는 다음과 같다. 만 5세 유아를 위한 지역사회 참여활동의 효과적 운영을 위해 지난 2년간 실행한 지역사회 참여활동을 분석하여 문제점을 파악 하고, 1차 실행을 계획 및 실시하였다. 1차 실행에서는 유아의 관심과 흥미를 반영한 옥상텃밭가꾸기 활동이 이루어졌으며 이를 통해 지역사회의 다양한 인적·물적 자원을 활용할 수 있었다. 또한 교실이라는 제한된 공간에서 벗어나 지역사회를 유아의 교육 공간으로 활용함으로서 지역사회 주민들과 적극적으로 상호작용이 이루어졌다. 2차 실행에서는 1차실행 평가를 토대로 공공기관 및 단체와 연계한 지역사회 참여활동을 실시하였다. 유아들은 일반사회의 중요한 내용인 공공기관 및 단체의 필요성을 이해할 수 있었다. 또한 지역사회 환경을 위해 유아들이 기여할 수 있는 활동을 실시하여, 유아들이 지역사회의 주체자로서 활동에 참여하는 기회를 가졌다. 이를 통해 지역사회를 구성하는 유아, 부모를 포함한 지역사회 주민들이 가로수길 살리기라는 공동의 목표를 가지고 함께 참여할 수 있었다. 이상의 연구 결과는 유아를 위한 사회교과 교육의 방법으로 지역사회 참여 활동의 필요성을 제기하였으며, 각 유아교육 현장에서는 지역사회의 특수성이 반영된 인적·물적 자원을 활용하여 다양한 형태의 지역사회 참여 활동을 제시하는 추후 연구가 이루어질 필요가 있음을 시사했다.

대장통과시간 측정용 방사선 비투과성 표지자인 KolomarkTM의 국내 개발

김지은,이풍렬,김영호,성인경,심상군,김재준,고광철,최규완,임효근,서수원 대한소화관운동학회 1999 Journal of Neurogastroenterology and Motility (JNM Vol.5 No.2

여성에서 Dehydroepiandrosterone sulfate와 인슐린저항성증후군의 연관성

김효정,홍은순,오지영,홍영선,성연아 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.5

연구배경: DHEA는 안드로겐 전구체로서 연령 증가에 따라 감소한다. 남성에서 DHEA는 인슐린저항성 및 심혈관질환에 대한 보호 작용을 하는 것으로 알려져 있으나 여성에서는 인슐린저항성과 심혈관질환에 대한 DHEA의 역할은 정립되어 있지 않다. 본 연구는 여성에서 인슐린저항성과 심혈관질환에서 DHEA의 역할을 규명하고자 시행하였다. 방법: 지역사회에서 무작위 추출된 471명의 여성을 대상으로 DHEA보다 반감기가 길고 일중 변동이 적은 것으로 알려져 있는 DHEAS의 농도를 방사면역측정법으로 측정하였다. DHEAS 농도와 인슐린저항성증후군 및 이 증후군을 구성하는 인자들과의 연관성을 관찰하였다. 결과: 1. 인슐린저항성증후군 인자의 빈도는 비만 25.3%, 내당능장애 8.5%, 고혈압 21.9%, 이상지질혈증 6.2%이었으며 인슐린저항성증후군의 빈도는 16.5%이었다. 2. DHEAS는 연령(r=-0.47, p<0.001), 수축기혈압(r=-0.18, p<0.001), 이완기혈압(r=-0.10, p<0.05), 공복혈당(r=-0.10, p<0.05), 포도당부하 2시간 혈당(r=-0.12, p<0.01), 및 중성지방(r=-0.16, p<0.01)과 의미있는 음의 상관관계가 있었다. 3. DHEAS 농도를 사분위수로 나눈 후 연령을 보정한 후 인슐린 저항성증후군 구성요소의 빈도를 관찰하였을 때 DHEAS 농도 감소에 따라 고혈압의 빈도가 유의하게 증가하였다.(p<0.05). 4. 연령(p<0.0001) 및 체질량지수(p<0.05)가 DHEAS에 영향을 주는 인자로 작용하였다. 5. 연령을 보정한 후 낮은 DHEAS농도는 인슐린저항성증후군의 위험인자로 작용하지 않았다. 결론: 여성에서 혈청 DHEAS 농도는 혈압, 혈당 및 혈청 지질 농도와 음의 상관관계가 있었으며, 연령을 보정한 후 DHEAS 농도가 감소함에 따라 고혈압의 빈도가 증가하는 것으로 보아 최소한 DHEAS는 인슐린저항성증후군에 대해 위해 작용을 가지지는 않는 것으로 생각된다. 그러나 DHEAS의 인슐린저항성증후군 및 심혈관질환에 대한 보호 작용을 증명하기 위해서는 이들 대상자를 전향적으로 추적 관찰하여 인슐린저항성증후군 및 심혈관질환의 발생과 DHEAS의 관계를 규명해야 할 것이다. Background: Dehydroepiandosterone (DHEA) is an androgen precursor, and is known to be decreased by the aging process. DHEA has been known to have a protective effect on insulin resistance and cardiovascular disease in men, but remains controversial in women. The aim of this study was to elucidate the role of DHEA on insulin resistance, and the risk for cardiovascular disease, in women. Methods: We analyzed the relationship between DHEA sulfate (DHEAS), known to have a longer half-life and less diurnal variation than DHEA, and insulin resistance syndrome (IRS) in 471 non-diabetic women from an urban community diabetes prevalence study. Serum DHEAS concentrations were measured using a commercially available radioimmunoassay kit. Results: 1. the frequencies of obesity, impaired glucose tolerance, hypertension and dyslipidemia were 25.3, 8.5, 21.9 and 6.2%, respectively, and the frequency of IRS was 16.5%. 2. DHEAS was significantly inversely correlated with age (r=-0.47, p<0.001), systolic blood pressure (r=-0.18, p<0.001), diastolic blood pressure (r=-0.10, p<0.05), fasting serum glucose (r=-0.10, p<0.05), postchllenge 2 hour glucose (r=-0.12, p<0.01) and triglycerides (r=-0.16, p<0.01). 3. As serum DHEAS concentrations, by quartiles, were decreased, the age-adjusted frequency of hypertension was significantly increase (p<0.05). 4. A Multiple linear regression analysis revealed that DHEAS was significantly associated with age (p<0.0001) and BMI (p<0.05). 5. A Logistic regression analysis showed that DHEAS was not associated with IRS after adjustment for age. Conclusion: DHEAS is inversely associated with age. DHEAS has no harmful effect, and may even have a protective role, on insulin resistance syndrome. Prospective examinations of DHEAS and insulin resistance syndrome in women are needed to confirm the mechanism for the association between DHEAS and the development of cardiovascular disease (J Kor Soc Endocrinol 17:675∼684, 2002).