MHC class Ⅰ분자들에 의해 제시되는 Epitope을 인지하는 CD8+ T림프구의 결핵균 감염에 대한 면역반응의 연구 : 결핵환자와 PPD+ 건강개체에 존재하는 결핵균 항원에 특정한 CD8+ T세포 Evidence of M. tuberculosis Specific CD8+ T Cells in TB Patients and PPD+ Healthy Individuals

조장은,이경화,박승규,천선희,조상래,조성애 Official Journal of the Korean society for immunol 2003 Immune Network Vol.3 No.3

Original Articles : Clinical significance of occult hepatitis B virus infection in chronic hepatitis C patients

( Jae Young Jang ),( Soung Won Jeong ),( Sung Ran Cheon1 ),( Sae Hwan Lee1 ),( Sang Gyune Kim ),( Young Koog Cheon ),( Young Seok Kim1 ),( Young Deok Cho1 ),( Hong Soo Kim ),( So Young Jin ),( Yun Soo 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: We investigated the frequency of occult hepatitis B virus (HBV) infection in anti-hepatitis C virus (HCV)-positive individuals and the effects of occult HBV infection on the severity of liver disease. Methods: Seventy-one hepatitis B virus surface-antigen (HBsAg)-negative patients were divided according to their HBV serological status into groups A (anti-HBc positive, anti-HBs negative; n=18), B (anti-HBc positive, anti-HBs positive; n=34), and C (anti-HBc negative, anti-HBs positive/negative; n=19), and by anti-HCV positivity (anti-HCV positive; n=32 vs. anti-HCV negative; n=39). Liver biopsy samples were taken, and HBV DNA was quantified by real-time PCR. Results: Intrahepatic HBV DNA was detected in 32.4% (23/71) of the entire cohort, and HBV DNA levels were invariably low in the different groups. Occult HBV infection was detected more frequently in the anti-HBc-positive patients. Intrahepatic HBV DNA was detected in 28.1% (9/32) of the anti-HCV-positive and 35.9% (14/39) of the anti-HCV-negative subjects. The HCV genotype did not affect the detection rate of intrahepatic HBV DNA. In anti-HCV-positive cases, occult HBV infection did not affect liver disease severity. Conclusions: Low levels of intrahepatic HBV DNA were detected frequently in both HBsAg-negative and anti-HCV-positive cases. However, the frequency of occult HBV infection was not affected by the presence of hepatitis C, and occult HBV infection did not have a significant effect on the disease severity of hepatitis C. (Korean J Hepatol 2011;17:206-212)

알레르기성 비염 환자에서 절지동물성 특이항원의 양성율 및 항원간 일치율

황규윤,남해선,박준수,권준택,백병준,김장묵,오천환,조성란 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

The current study was designed to evaluate the positive rates of arthropod allergens by Korean inhalant panel of MAST-CLA in patients with allergic rhinitis. One hundred sixty patients, diagnosed to allergic rhinitis in an Out Patient Center of Soonchunhyang University Chunan Hopsital during Aug 1998 to July 2000, were studied. 1. The overall positive rate among 35 specific allergens was 59.4% (95 patients). 2. The arthropod related allergen accounted for 82.1% (78 patients) of the positive rate. 3. Each positive rate of Dermatophagoides farinae (Df), Dermatophagoides pteronyssinus (Dp), house dust (Hd), and cockroach mix (Cm) was 45.0% (72 patients), 43.1% (69 patients), 28.1% (45 patients), and 8.8% (14 patients), respectively. 4.Significant agreement between arthropod allergens was observer (P < 0.05). 5. The obseved agreement between Df and Dp was 95.6%, showing most significant Kappa index (Kappa = 0.911, P < 0.001). There results suggested that although MAST-CLA is a useful measure of identifying specific allergens, the specificity of two mite allergen relatively low, implying because of common allergen between mites in Korean panel of MAST-CLA. Our data also supported that arthropod is the major cause of allergen in patients with allergic rhinitis, however, the MAST-CLA should be modified in order to exclude common allergen of mite and to include more specific mite allergen.

Direct Extraction of DNA from Soil for Amplification of 16S rRNA Gene Sequences by Polymerase Chain Reaction

Cho, Jae-Chang,Lee, Dong-Hun,Cheol, Cho-Young,Cho, Jang-Cheon,Kim, Sang-Jong The Microbiological Society of Korea 1996 The journal of microbiology Vol.34 No.3

Microgram quantities of DNA per gram soil were recovered with SDS- based and freeze-and thaw procedures. The average DNA fragment size was > 23 Kb. This method generated minimal shearing of extracted DNA. However, the DNA extracts still contained considerable amounts of humic impurities sufficient to inhibit PCR. Several approaches were used to reduce the interferences with the PCR (use of CTAF in extraction step, Elutip-d column purification, addition of BSA to PCR buffer) to accomplish PCR with DNA extract as a template. Most of the DNA extracts were not digested completely by restriction endonuclease, and CTAB-TREATED ane Elutip-d column purified DNA extracts were partially digested. Regarding as restriction enzyme digestion, all PCRs failed to amplify 16S rRNA gene fragments in the DNA extracts. In the case of DNA extracts only where BSA was added to PCR buffer, PCR was successfully conducted whether the DNA extracts were treated with CTAB or purified with columns. However, these two treatments were indispensable for humic impurity-rich DNA extracts to generate the PCR-compatible DNA samples. Direct extraction of DNA, coupled with these procedures to remove and relieve interferences by humic impurities and followed by the PCR, can be rapid and simple method for molecular microbiological study on soil microorganisms.

A report of 17 unrecorded bacterial species of Korea belonging to the phylum Bacteroidetes

Cho, Jang-Cheon,Seong, Chi Nam,Joh, Kiseong,Cha, Chang-Jun,Bae, Jin-Woo,Yi, Hana,Lee, Soon Dong,Kim, Myung Kyum,Yoon, Jung-Hoon The National Institute of Biological Resources 2018 Journal of species research Vol.7 No.3

While screening indigenous prokaryotic species in Republic of Korea in 2017, a total of 17 bacterial strains assigned to the phylum Bacteroidetes were isolated from a variety of environmental habitats including water of fountain, tidal flat, plant root, soil, the gut of Russian grayling butterfly, ginseng field, seawater, lagoon and seashore sand. From the 16S rRNA gene sequence similarity of more than 98.7% and the formation of a robust phylogenetic clade with the closest species, it was found that the 17 strains belong to independent and recognized bacterial species. There has been no official report that the identified 17 species have been previously isolated in the Republic of Korea. Thus, 15 species in 10 genera of one family in the order Flavobacteriales, one species in one genus of one family in the order Cytophagales, and one species in one genus of one family in the order Sphingobacteriales are proposed as unrecorded species of the phylum Bacteroidetes found in the Republic of Korea. Gram reaction, colony and cell morphology, basic phenotypic characteristics, isolation source, taxonomic status, strain ID and other information are described in the species descriptions.

Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales.

Cho, Jang-Cheon,Giovannoni, Stephen J Society for General Microbiology 2006 International journal of systematic and evolutiona Vol.56 No.4

<P>A Gram-negative, chemoheterotrophic, facultatively anaerobic, slightly halophilic, oval-shaped marine bacterium, designated HTCC2601T, was isolated from the western Sargasso Sea by high-throughput culturing involving dilution to extinction. Although the 16S rRNA gene sequence similarity between the isolate and Salipiger mucosus was 96.5 %, phylogenetic analyses using different treeing algorithms clearly indicated that the strain forms a distinct lineage within a clade containing the recently classified genera Salipiger and Palleronia in the order Rhodobacterales of the Alphaproteobacteria. The DNA-DNA relatedness between strain HTCC2601T and S. mucosus was 26.3 %. Strain HTCC2601T utilized a wide range of carbohydrates, including hexose monomers, sugar alcohols, organic acids and amino acids, as sole carbon sources. The DNA G+C content of strain HTCC2601T was 65.4 mol%, and the predominant constituents of the cellular fatty acids were 18 : 1omega7c (79.7 %) and 11-methyl 18 : 1omega7c (7.5 %). The strain differed from members of the closely related genera Salipiger and Palleronia in its morphological, biochemical and ecological characteristics. On the basis of the taxonomic data obtained in this study, a novel genus and species, Pelagibaca bermudensis gen. nov., sp. nov., is proposed; HTCC2601T (=KCTC 12554T = JCM 13377T) is the type strain of Pelagibaca bermudensis.</P>

Polyphyletic photosynthetic reaction centre genes in oligotrophic marine <i>Gammaproteobacteria</i>

Cho, Jang-Cheon,Stapels, Martha D.,Morris, Robert M.,Vergin, Kevin L.,Schwalbach, Michael S.,Givan, Scott A.,Barofsky, Douglas F.,Giovannoni, Stephen J. BLACKWELL SCIENCE 2007 ENVIRONMENTAL MICROBIOLOGY Vol.9 No.6

<P>Summary</P><P>Ecological studies indicate that aerobic anoxygenic phototrophic bacteria (AAP) that use bacteriochlorophyll to support phototrophic electron transport are widely distributed in the oceans. All cultivated marine AAP are alpha-3 and alpha-4 <I>Proteobacteria</I>, but metagenomic evidence indicates that uncultured AAP <I>Gammaproteobacteria</I> are important members of ocean surface microbial communities. Here we report the description of obligately oligotrophic, marine <I>Gammaproteobacteria</I> that have genes for aerobic anoxygenic photosynthesis. Three strains belonging to the OM60 clade were isolated in autoclaved seawater media. Polymerase chain reaction assays for the <I>puf</I>M gene show that these strains contain photosynthetic reaction centre genes. DNA sequencing and phylogenetic analysis indicate that the <I>puf</I>M genes are polyphyletic, suggesting multiple instances of lateral gene transfer. Peptide sequences from six photosynthesis genes (<I>puf</I>L, <I>puf</I>M, <I>puf</I>C, <I>puf</I>B, <I>puf</I>A and <I>puh</I>A) were detected by proteomic analyses of strain HTCC2080 cells grown aerobically in seawater. They closely match predicted peptides from an environmental seawater bacterial artificial chromosome clone of gammaproteobacterial origin, thus identifying the OM60 clade as a significant source of gammaproteobacterial AAP genes in marine systems. The cell yield and rate of growth of HTCC2080 in autoclaved, aerobic seawater increased in the light. These findings identify the OM60 clade as a source of <I>Gammaproteobacteria</I> AAP genes in coastal oceans, and demonstrate that aerobic, anoxygenic photosynthetic metabolism can enhance the productivity of marine oligotrophic bacteria that also grow heterotrophically in darkness.</P>

Efficacy and Safety of Sipjeondaebo-Tang for Anorexia in Patients with Cancer: A Pilot, Randomized, Double-Blind, Placebo-Controlled Trial

Cheon, Chunhoo,Yoo, Jeong-Eun,Yoo, Hwa-Seung,Cho, Chong-Kwan,Kang, Sohyeon,Kim, Mia,Jang, Bo-Hyoung,Shin, Yong-Cheol,Ko, Seong-Gyu Hindawi 2017 Evidence-based Complementary and Alternative Medic Vol.2017 No.-

<P><B>Background</B></P><P> Anorexia occurs in about half of cancer patients and is associated with high mortality rate. However, safe and long-term use of anorexia treatment is still an unmet need.</P><P><B> Objective</B></P><P> The purpose of the present study was to examine the feasibility of Sipjeondaebo-tang (Juzen-taiho-to, Shi-Quan-Da-Bu-Tang) for cancer-related anorexia.</P><P><B> Methods</B></P><P> A total of 32 participants with cancer anorexia were randomized to either Sipjeondaebo-tang group or placebo group. Participants were given 3 g of Sipjeondaebo-tang or placebo 3 times a day for 4 weeks. The primary outcome was a change in the Anorexia/Cachexia Subscale of Functional Assessment of Anorexia/Cachexia Therapy (FAACT). The secondary outcomes included Visual Analogue Scale (VAS) of anorexia, FAACT scale, and laboratory tests.</P><P><B> Results</B></P><P> Anorexia and quality of life measured by FAACT and VAS were improved after 4 weeks of Sipjeondaebo-tang treatment. However, there was no significant difference between changes of Sipjeondaebo-tang group and placebo group.</P><P><B> Conclusions</B></P><P> Sipjeondaebo-tang appears to have potential benefit for anorexia management in patients with cancer. Further large-scale studies are needed to ensure the efficacy.</P><P><B> Trial Registration</B></P><P> This trial is registered with ClinicalTrials.gov NCT02468141.</P>

Korean Red Ginseng Extract Attenuates 3-Nitropropionic Acid-Induced Huntington's-Like Symptoms

Jang, Minhee,Lee, Min Jung,Kim, Cheon Suk,Cho, Ik-Hyun Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Korean red ginseng (KRG) possesses neuroprotective activity. However, the potential neuroprotective value of KRG for the striatal toxicity is largely unknown. We investigated whether KRG extract (KRGE) could have a neuroprotective effect in a 3-nitropropionic acid- (3-NP) induced (i.p.) Huntington's disease (HD) model. KRGE (50, 100, and 250 mg/kg/day, p.o.) was administrated 10 days before 3-NP injection (pre-administration), from the same time with 3-NP injection (co-administration), or from the peak point of neurological impairment by 3-NP injection (post-administration). Pre-administration of KRGE produced the greatest neuroprotective effect in this model. Pre-administration of KRGE significantly decreased 3-NP-induced neurological impairment, lethality, lesion area, and neuronal loss in the 3-NP-injected striatum. KRGE attenuated microglial activation and phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-<I><I>κ</I></I>B) signal pathway. KRGE also reduced the level of mRNA expression of tumor necrosis factor-alpha, interleukin- (IL-) 1<I><I>β</I></I>, IL-6, inducible nitric oxide synthase, and OX-42. Interestingly, the intrathecal administration of SB203580 (a p38 inhibitor) or PD98059 (an inhibitor of MAPK Kinase, MEK) increased the survival rate in the 3-NP-induced HD model. Pre-administration of KRGE may effectively inhibit 3-NP-induced striatal toxicity via the inhibition of the phosphorylation of MAPKs and NF-<I><I>κ</I></I>B pathways, indicating its therapeutic potential for suppressing Huntington's-like symptoms.</P>

Incidental Focal $^{18}F$-FDG Uptake in the Prostate: Clinical Significance and Differential Diagnostic Criteria

Cho, Suk-Kyong,Choi, Joon-Young,Yoo, Jang,Cheon, Mi-Ju,Lee, Ji-Young,Hyun, Seung-Hyup,Lee, Eun-Jeong,Lee, Kyung-Han,Kim, Byung-Tae The Korea Society of Nuclear Medicine 2011 핵의학 분자영상 Vol.45 No.3

Purpose The extent and intensity of $^{18}F$-FDG uptake in prostate cancer patients are known to be variable, and the clinical significance of focal $^{18}F$-fluorodeoxyglucose (1$^{18}F$-FDG) uptake that is incidentally found on positron emission tomography (PET) has not been established. We investigated the clinical significance of incidental focal prostate uptake of $^{18}F$-FDG on PET/computed tomography (CT) and analyzed differential findings on PET/CT between malignant and benign uptake. Methods A total of 14,854 whole-body $^{18}F$-FDG PET/CT scans (4,806 that were conducted during cancer screening and 10,048 that were conducted to evaluate suspected or alleged cancer outside of the prostate) were retrospectively reviewed to determine the presence, location, multiplicity and maximum standardized uptake value (SUVmax) of focal prostate uptake and combined calcification. The final diagnosis determined by serum prostate-specific antigen (PSA) level and biopsy was compared with PET findings. Results Incidental focal prostate uptake was observed in 148 of 14,854 scans (1.0 %). Sixty-seven of these 148 subjects who had diagnostic confirmation were selected for further analysis. Prostate cancer was diagnosed in nine of 67 subjects (13.4%). The remaining 58 subjects had no malignancy in the prostate based on normal serum PSA level (n=53), or elevated serum PSA level with a negative biopsy result (n=5). While 84.6% (11/13) of malignant uptake was peripherally located in the prostate glands, 60.2% (50/83) of benign uptake was centrally located (p<0.05). The positive predictive value of peripheral focal uptake for malignancy was 25%. The SUVmax, multiplicity and combined calcification were not significantly different between the two groups. Conclusion Although incidental focal $^{18}F$-FDG uptake in the prostate is not common, the incidence of cancer with focal uptake is not low. Therefore, these findings deserve further evaluation.The location of the focal prostate uptake may help with the selection of high-risk prostate cancer patients.