두 가족의 가족성 모야모야병 환자 4례

구자성,노재규,박성호,이한보,이용석,윤창호,송치성,송재우 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

노인 환자에서의 신경계 응급 질환

구자성 대한노인병학회 2003 대한노인병학회지 Vol.7 No.3

폐암에서 CYFRA 21-1과 다른 종양표지자의 진단적 의의

이상구,이호현,전병철,김성자,이영현,김문연,하경임 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

폐암의 확실한 조직학적 진단이 어려울 경우 종양표지자의 검사가 보조적인 진단 수단이 되고, 조기 진단이나 경과 관찰에 이용되고 있다. 이에 저자는 CYFRA 21-1이 폐암의 종양표지자로서 효용성이 있는지 판정하고, CYFRA 21-1과 다른 폐암 종양표지자인 SCC Ag, CEA, NSE의 폐암의 조직학적 유형에 따른 민감도와 특이도를 비교하였다. 그리고 병기 진행에 따른 CYFRA 21-1치의 증가 여부를 관찰하고, 4가지 종양표지자를 동시에 측정하였을 때의 진단적 효용성을 비교하고자 하였다. 이 연구는 1994년 12월부터 1995년 11월까지 동국대학병원에 입원한 폐암 환자 40명과 양성 폐질환 환자 40명을 대상으로 하였다. 편평상피세포암 21명, 선암 10명, 소세포암 7명, 대세포암 2명이었다. 혈청 CYFRA 21-1의 cytokeratin 19 분절에 대한 쥐의 두 가지 단일 클론항체(KS 19-1과 BM 19-21)를 이용하는 RIA방법으로 측정하였다. CEA는 MEIA 방법으로, SCC 항원과 NSE는 RIA로 측정 하였다. 1. CYFRA 21-1의 혈중 농도는 폐암군 22.08±43.00ng/mL, 양성 폐질환군 1.14±1.04ng/mL로 폐암군에서 양성 폐질환군보다 유의하게 높았다(P<0.05). 폐암환자군에서 55%의 양성율을 보였고, SCC 항원 30%, CEA 44.7%, NSE 54.5%의 양성율을 나타내 CYFRA 21-1이 가장 높은 양성율을 보였다. 2.CYFRA 21-1은 비소세포암군 23.79±44.49ng/mL, 소세포암군 13.90±33.57ng/mL로 양군에서 유의한 차이를 보였다. 폐암환자군에서 민감도와 특이도는 CYFRA 21-1 55.0%와 96.7%, SCC항원 30.0%와 96.4%, CEA 42.5%와 92.8%, NSE 36.4%, 76.9%로 CYFRA 21-1에서 가장 높은 민감도와 특이도를 보였다. 폐암의 조직학적 유형에 따른 민감도는 편평상피세포암에서 CYFRA 21-1이 61.9%로 CYFRA 21-1의 민감도가 가장 높았고, 선암에서는 CEA가 88.8%로, 소세포암에서는 NSE가 85.7%로 가장 민감도가 높은 것으로 나타났다. 3. 비소세포암군에서 CYFRA 21-1치는 병기가 진행됨에 따라 증가하는 경향을 보였으나 통계학적으로 유의하지는 않았다. 4. 폐암환자에서 단일 종양표지자를 측정하는 것 보다 동시에 여러 종양표지자를 측정하는 것이 특이도는 떨어지나 민감도는 80.0%로 더 높은 것으로 나타났으며 정확도에는 큰 차이가 없었다. 양성 표지나 수에 따른 비교분석시 폐암의 상대 예측도는 두 표지자 양성인 경우가 76.5%, 3가지 표지자와 4가지 모든 표지자에서 양성으로 나온 경우는 100%로 나타났다. 본 연구 결과 CYFRA 21-1은 새로운 폐암의 종양표지자로 기존의 종양표지자 보다 민감도가 높고 비소세포암 특히, 편평상피세포암에 유용한 표지자로 사료되며, 폐암이 의심되는 환자에서 단일 종양 표지자를 측정하는 것 보다 수종의 종양표지자를 동시에 측정하는 것이 진단에 더욱 도움이 되리라 생각된다. Background: Cytokeratin 19 is a subunit of cytokeratin intermediate filament expressed in simple epithelia and their malignant counterparts. A fragment of cytokeratin subunit 19 can be measured in serum with a immunoradiometric assay using two mouse MoAb KS 19-1 and BM 19-21. Thus this cytokeratin 19 fragment is referred to as CYFRA 21-1. The aims of this study are to evaluate the clinical utility of CYFRA 21-1 in the diagnosis of lung cancer and to compare the diagnostic sensitivity and specificity of CYFRA 21-1 with those of CEA, SCC Ag, NSE according to histological type of lung cancer. Methods: In 40 patients with lung cancer(21 with squamous cell carcinoma, 10 with adenocarcinoma, 7 with small cell carcinoma, 2 with large cell carcinoma) and 40 patients with non-malignant lung disease, serum CYFRA 21-1 was measured by solid-phase immunoradiometric assay(CIS Bio International, France). Serum NSE and SCC Ag were measured by immunoradiometric assay, and CEA was measured by microparticle enzyme immunoassay. Results: 1) The mean value of CYFRA 21-1 was 22.08±43.00ng/mL in the lung cancer and 1.11±1.04ng/mL in me non-malignant lung disease group(P<O.O5). 2) Using the cut-off value of 3.3ng/mL, the sensitivity and specificity of CYFRA 21-1 were 55.0%, 96.7% in the lung cancer. The sensitivity of CYFRA 21.1 was 61.9% in squamous cell carcinoma 3) The level of CYFRA 21-1 was increasing tendency with the progression of stage in non-small cell carcinoma but statistically not significant. 4) Simultaneous determination of four tumor markers revealed increased sensitivity to 50.0% in lung cancer. As the number of positive markers was increased, the relative possibility of lung cancer was also increased. If two markers were positive, it increased to 76.5% and three markers were positive, it increased to 100%.5 Conclusions: CYFRA 21-1 is a useful serum marker for patients with lung cancer, especially in squamous cell carcinoma of the lung. The simultaneous measurement of CYFRA 21-1, CEA. SCC Ag and NSE would provide additional information for the diagnosis of lung cancer, especially in patients with high risk group of lung cancer.

Cefradine 함유 alginate bead의 제조 및 용출

김대섭,구자성,양재헌 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

Cefradine, first generation cephalosporins, is a water soluble antibiotic agent. We prepared the alginate beads containing cefradine for developing release and evaluated the dissolutions of cefradine from beads. Cefradine was encapsulated into inner water phase of W/O/W emulsion beads which were prepared by cross-linking of alginate with Ca^2+. The delayed release patterns of cefradine for W/O/W emulsion beads were observed as it was compared with alginate simple beads without oil phase. As a results, W/O/W multiple emulsion beads could be use a controlled release DDS of water soluble agents such as cefradine. Also chitosan of high M.W could be a good intermediator of controlled release DDS.

혈관성 파킨슨증의 경사에코 자기공명촬영 소견 : 환자-대조군 연구 A Case-Control Study

배희준,김병건,구자성,김현숙 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.2

Background & objective : Changes of signal intensity in the various Structures of basal ganglia(BG), Which can be detected by gradient echo MRI(GE-MRI). have been reported in different kinds of parkinsonism Altered iron content in these structures may be a possible explanation of above phenomenon Vascular parkinsonism is a kind of parkinsonism syndrome and is common However. There have been no reports about the BG signal changes on GE-MRI in vascular parkinsonism Methods : We investigated the difference of signal intensity in various BG and midbrain structures between 36 patients with vascular parkinsonism. which was defined as clinical parkinsonism+evidence of relevant vascular lesion on MRI+poor or no response to levodopa, and age-& sex- matched controls. The intensity of BG on GE-MRI was graded as 0-3 relative to cortical gray matter Results : There were no significant differences in clinical characteristics between two groups Among patients with vascular parkinsonism, hypointensity was observed in globus pallidus in 94.4%. in putamen in 58.3% in head of caudate in 33.3%, in substantia nigra in 72.2%,& in red nucleus in 52.8% There was no significant linear trend in the association between signal intensity of above structures and the presence of vascular parkinsonism (p>0 05 on Mantel-Haenszel chi-square test) However, the correlation between the degree of white matter lesions and the presence of vascular parkinsonism was statistically significant (p=003). Conclusions : Our GE-MRI study fails to prove that iron content of various BG structures in patients with vascular parkinsonism is different from controls.

한국인 편두통 환자와 미토콘드리아 DNA 11,084 유전자 다형성의 연관성

김병건,배희준,구자성,권오현,김만호 대한두통연구회 2002 두통 Vol.3 No.2

병력청취에 의한 양성 발작성 체위성 어지럼증의 진단

김병건,배희준,구자성,권오현 대한평형의학회 2003 Research in Vestibular Science Vol.2 No.2

동백종실 및 유박의 식품으로서의 이용성 검토

김영환,정현숙,김용두,최옥자,강성구 順天大學校 師範大學 附屬 科學敎育硏究所 1997 科學과 敎育 Vol.5 No.-

동백종실의 이용성을 검토하기 위해서 NaCl(1.5%), ethanol(99.9%), blanching의 방법으로 쓴맛을 제거하여 관능검사를 한 결과는 다음과 같다. 1. 동백종실의 쓴 맛을 제거하는데는 NaCl용액보다는 알콜이 더 효과적이었으며, blanching, NaCl용액 및 알코올을 병행처리 하였을때 쓴맛이 잘 제거되었다. 그러나 동백종실은 알맹이로 되어 있어 쓴맛의 제거에 많은 시간이 소요되고, 완전하게 쓴맛이 제거되지 않으므로 종실자체를 식품으로 이용하는 것은 문제점이 있다고 생각된다. 2. 동백유박의 쓴맛제거는 증류수와 NaCl용액에서도 쓴맛이 거의 제거되었으며, 알콜의 경우는 30% 농도일 때 가장 효과가 좋은 것으로 나타났다. 동백유박은 동백종실에 비하여 쓴맛의 제거가 훨씬 더 용이하였으며, 식품재료로서의 이용가능성에 대한 기호도가 높은 것으로 나타나 이를 이용한 식품의 개발은 가능하다고 생각된다. This study was carried out to investigate the utilization of Camellia seed and defatted Camellia seed flour as food. We removed bitter taste of Camellia seed and defatted Camellia seed flour by using NaCl solution, ethanol, and blanching method. The following are the results of a sensory evaluation. 1. It was bitter taste rather than color or flavor which has made a great effect on the possibility of utilization for food materials. Alcohol was more effective than NaCl solution in removing bitter taste of Camellia seed, while bitter taste was easily removed when the processing with blanching, NaCl solution, and alcohol was employed. Since Camellia seed was consisted of grain, however, much time was needed to remove bitter taste. Because bitter taste wasn't perfectly removed, it has a problem in using Camellia seed for food materials. 2. Bitter taste of the defatted Camellia seed flour was almost removed in the distilled water, NaCl solution, and alcohol with a variety of concentrations. When various concentrations of alcohol were used, 30% concentration worked best, Bitter taste of defatted Camellia seed flour was removed more easily than that of Camellia seed. In conclusion, it is thought to be possible to develop into food, because of a high possibility of utilization as food materials

정보제공자와의 면담에 의해 진단된 뇌졸중후치매의 유병률과 상관인자에 관한 연구

배희준,이건세,김형수,김병건,구자성,권오현,박종무 대한치매학회 2004 Dementia and Neurocognitive Disorders Vol.3 No.2

Background and Objectives: With increasing age of population, stroke and dementia become greater health problems in Korea. However, there have been no studies on poststroke dementia in Korea. We intended to elucidate the frequency and clinical correlates of poststroke dementia in a hospital-based cohort. Methods: From July 2001 to July 2002, 372 patients were hospitalized to Eulji General Hospital within 7 days from onset with acute stroke or transient ischemic attack. Two hundred-eight patients (55.9%) were followed up and interviewed more than 1 year later (451 ±83.3 days). Dementia was diagnosed by Korean Dementia Screening Questionnaire (KDSQ) obtained by a direct interview. Based on Eulji Stroke Registry, demographic factors, risk factors for stroke, stroke characteristics, and stroke outcomes were gathered and examined. Results: Of 208 patients, poststroke dementia was detected in 36 patients (17.3%, 20 males, age=64.5±9.4 years). Poststroke dementia was associated with history of stroke, aphasia, modified Rankin Disability Scale (MRDS) at 3 month after stroke and when interviewed, and Barthel index at 6 months after stroke (p;0.05). Its correlation with diabetes mellitus and National Institute of Health Stroke Scale at discharge were marginally significant (0.05;p;0.1). Conclusions: Post-stroke dementia is common in Korean stroke patients. Its frequency is comparable to that in other countries. The correlates of poststroke dementia and their clinical meanings are demonstrated.

Treatment with ionomycin improves pregnancy rates in poor responders

( Ja-seong Koo ),( Sung-eun Moon ),( Hyeyoung Lee ),( Hwa-sook Moon ),( Sung-hun Min ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: Ionomycin can induce Ca2+ influx by altering the plasma membrane permeability or can act directly on intracellular organelles which release Ca2+. Studies in somatic cell nuclear transfer bovine oocytes showed that ionomycin is a more potent and more specific Ca2+ ionophore than A23187. When ionomycin is used as an assisted oocyte activation agent, the reported fertilization rates are usually higher than with the use of A23187. In humans, calcium fluctuations were detected with a peak shortly before cell division. Interestingly, these calcium oscillations disappeared in arrested embryos. 방법: This prospective study was performed between October 2014 and December 2015 at a reproductive center. All patients involved gave written consent, and institutional review board approval was granted. This study includes 21 non-treatment and 22 treatment couples. A prospective study was conducted including patients with a poor responders following conventional ICSI in our center. In the treatment cycles, all metaphase II-oocytes were exposed to a commercially available ready-to-use 0.5 μM ionomycin for 5 min immediately after ICSI. After a three-step washing procedure, in vitro culture was performed as in the control cycles, when possible up to 8-cell stage. Cleavage and clinical pregnancy rates were compared, p-value of <0.05 was considered statistically significant. 결과: The average female age, number of oocytes retrieved, embryo developmental stage and number of embryos transferred did not vary significantly amongst the groups. Fertilization rate did not differ (75.4 versus 73.2%); however, further cleavage to 8-cell stage was significantly higher (p<0.001) in the ionomycin group (98.5%) when compared with the control cycles (91.9%). Most importantly, clinical pregnancy rates were higher (p<0.01) in the ionomycin treatment group (44.4%) when compared to the control group (20.8%). 결론: The findings of this study indicate that ionomycin treatment improves the rates of cleavage to 8-cell stage and clinical pregnancy in poor responders. However, this treatment does not seem to completely resolve the poor responders. Further investigations are necessary to determine the effects of ionomycin treatment of the culture conditions.