Random Amplified Polymorphic DNA Typing에 의한 동물과 사람 유래 Vancomycin 내성 장구균의 유전학적 비교

조윤상,이희수,김종만,류판동,박용호,유한상,이문한 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

It has been recently reported the possibility in the transfer of antimicrobial resistant to other animals and humans. In particular, the occurrence of vancomycin resistant enterococci (VRE), which have been known as a principal antimicrobial resistant bacteria in humans, has been increased as a pathogen of nosocomial infections. And then animal VRE were suspected as an origin of human VRE. In this work, we investigated the relatedness between VRE from animals and humans through random amplified polymorphic DNA (RAPD) typing. The genetic relatedness of vanA, vanB, vanC-1 and vanC-2 genotypes was examined by RAPD typing, respectively. van4 genotypes have not been shown high genetic relationship each other, containing VRE from humans (n = 8) and chickens (n = 5). In vanB genotypes from humans (n = 3), 2 isolates were made up one cluster, shown 77.8% homology. Chicken isolates of vanC-1 genotypes (n = 11) were constituted 2 clusters and the homology of 2 clusters was 81.8% and 80.0%, respectively. In case of vanC-2 genotypes (n = 19), 2 isolates from pigs have been shown 76.9% homology. But, between VRE from animals and humans, the isolates of high genetic relationship could not be found and RAPD typing was a useful epidemiological method that could be confirmed the genetic relationship among VRE.

骨髓纖維化症으로 移行된 骨髓異形成症候群

이동순,황유성,조한익,김노경,김병국 大韓血液學會 1985 大韓血液學會誌 Vol.20 No.2

성남시 일부 성인의 건강지각과 건강행위에 관한 연구

한애경,조동숙 한국전문대학교육연구학회 2001 한국전문대학교육연구학회논문집 Vol.2 No.2

The purpose of this study was to investigate the health perception and health behavior of adults in Sung Nam area, and the correlation between this variable. The subjects were 354 adults who participated voluntary in health examination program. The instrument for this study were health perception scale and health practice performance scale. Health perception scale were developed by Ware(1976) and the health practice performance scale developed by Chang et al(1999). The results of tills study as follows: 1. Of a possible maximum score of 44 points on the 11 question about health perception, the mean score of health perception was 28.21±5.23. And of a possible maximum score of 26 points on the 13 question about health behavior. the mean score of health behavior was 17.58±2.27. So it was found that the level of health perception and health behavior of adults in Sung Nam area was low. 2. Health perception and Health behavior were showed statistically positive correlation (r=.184.p=.000). 3. According to the result of multiple regression analysis, health perception and general charactenstics(age, vocation, religion) predicted 13.9% of health behavior.

임상재료에서 분리된 녹농균(綠膿菌)의 혈청형과 약제감수성(藥劑感受性)에 대하여

조양자,한옥수,이동후 대한미생물학회 1976 大韓微生物學會誌 Vol.11 No.1

Two hundred and fourty eight strains of Pseudomonas aeruginosa isolated from clinical materials at Department of Bacteriology in National Medical Center and Han-iI Hospital during January to November in 1973, were typed serologically by Hommos agglutination method utlizing a routine set of 13 standard sera. In addition, their susceptiblity to several kinds of antibiotics were determind. The following results were obtained; One hundred seventy eight strains (71,77%) were typable with an occurrence of type T5 in 41 strains (16. 58%), type T5, in 36 (14.52%), type T3, in 24 strains (9. 68%) and smalI numbers of strains were distributed in other types. Seventy strains (28. 23%) were nontypable. The rate of isolation of Pseudomonas by clinical meterials was shown as 49. 19% in ous, 16. 53% in sputum and 8. 87% in urine; the isolation rate of 1. 21-3. 15% was shown in other clinical meteraIs and the definite distribution rate could not be observed in the serotype by different materials. Majorities of strains used in this experiment of isolates were resistant to common antibiotics but Gentamycin and Carbenicillin, known relativeIy as sensitive antibiotics to Pseudomonas aeruginosa, were observed resistance of 2. 44-10. 5% and 16. 69-57. 8%. Moreover any particular relationship between serotype and the sensitivity of antibiotics was not identified.

한국인에서 검출된 C형 간염바이러스의 외피 (E2/NSl)유전자 염기 배열 분석

이동순,조한익 대한임상병리학회 1997 대한임상병리학회지 Vol.17 No.5

Experimental study on condensation heat transfer characteristics of special heat transfer tubes

한규일,박종운,권영철,조동현 대한설비공학회 2001 설비공학 논문집 Vol.13 No.9

In this study, condensation heat transfer characteristics were conducted with special heat transfer tubes of SH-C type. Experiments were carried out the saturated vapor temperature of 334K and the wall subcooling of 1.5-4.5K. The refrigerant was R-113 and the enhanced tubes used in the present study were SH-CDR, SH-CYR and SH-CHR. The experimental results showed that the condensation heat transfer coefficients of SH-C type tubes were about 23-66% higher than those of a low integral-fin tube. It was visualized that the condensed liquid on the outer surface of SH-C type tubes flowed continuously down unlike a low integral-fin tube and a plain tube, due to a 3-D extending fin on the outer surface of SH-C type tubes. As a result, the thermal resistance of the condensed liquid decreased and the heat transfer coefficient increased. Also, the enhancement ratio of SH-CDR tube was the highest, and it was about 9-11 times as compared to that of a plain tube.

노인의 식습관과 건강상태에 관한 연구 : 부산시 남ㆍ녀 노인을 중심으로 the Elderly Man and Woman in Pusan

조경자,한동희 慶星大學校 1994 論文集 Vol.15 No.2

This study was undertaken to investigate the relationship between the food habits and the health status. For this purpose, data were collected from deep interviews with 124 the elderly women and men, age 65 or greater in Pusan, Korea. For the data analysis, percentages, frequencies and means were calculated in order to describe the data. t-test, ANOVA, χ²-test were used. As a result of the data anylsis, the following results were obtained : 1. The age about the object person of research was the most numerous in 79% at 65-74 years old, in 70.9% with any their children, most elderly women and men were without job, regular excercise, regular health check and regular income. 2. The average man's weight was 61.89±6.33kg, woman's weight was 52.75±4.42kg, the average man's height was 166.47±7.92cm, woman's height was 154.96±6.02cm. 3. Both of the elderly men and women had regular meals at breakfast, lunch and supper. The most delicious meal was supper. The style of food intake was vegetables or mixed with vegetables and meats. Eventhough the participating at eating out with family was low, having meals with family at home usually. The problem of food habits was too much salty. 4. Eventhough both of the elderly men and women had eating some snacks between meals, they didn't have any nutritious reasons. Usually their child supplied it and in case of spouse, only the elderly women had the charge. 5. The elderly men were higher than the elderly women in case of smoking and drinking. 6. The elderly men wee better score than the elderly women with food habits. 7. Most of the elderly men and women had any chronic illness. 8. The elderly men were higher score with most areas except respiratory organs, the elderly women were score than the elderly men in the state of health. 9. The relationship between the elderly men and women showed a meaningful difference, fatigue showed a symptom as physical health, and nervousness showed a symptom as mental health with having poor food habits. The chronic illness also connected with poor food habits.

燐酸施肥量 差異가 靑刈豌豆의 主要形質 및 收量에 미치는 影響

趙男棋,韓英明,朴良門,高東煥 濟州大學校 亞熱帶農業硏究所 1998 亞熱帶農業硏究 Vol.15 No.-

本 硏究는 濟州道에서 燐酸施肥量差異가 靑刈豌豆의 主要 形質과 收量에 미치는 影響을 究明하기 위하여 濟州在來豌豆와 Sparkle을 供試하여 1998年 3月부터 5月까지 修行하였으며 試驗한 結果를 要約하면 다음과 같다. 1. 開花日數는 燐酸施肥量이 많아짐에 따라 늦어지는 傾向이었다. 品種問의 開花日數는 濟州在來豌豆가 빨랐고, Sparkle은 늦은 편이었다. 2. 草長은 燐酸施肥量이 많을수록 길었으며, 品種間에는 各 處理區에서 濟州在來豌豆가 길었고, Sparkle은 짧았다. 3. 莖直經, 主莖節數는 濟州在來豌豆 Sparkle 두 品種 供히 燐酸施肥量이 增加할수록 增加하였다. 4. 10a當 靑刈收量은 두 品種 모두 燐酸施肥量이 많아짐에 따라 漸次的으로 增加되는 傾向이었다. 5. 濟州求來談臺의 草長과 靑刈收量과는 高度로 有意한 正의 相關을, Sparkle은 正의 相關關係가 있어서 두 品種 모두가 草長이 길수록 靑刈收量이 많았다. Two pea cultivars, 'Cheju local pea' and 'Sparkle', were planted on 3 March at Cheju at 5 phosphate rates(0, 5, 10, 15, 20kg/10a) to determine the optimum phosphate fertilization rate. Days to flowering increased as phosphate rate was increased. Days to flowering of Cheju local pea was earlier than that of Sparkle. The more phosphate rate, the higher plant height Plant height of cultivar Cheju local pea was taller than of Sparkle. As phosphate rate was increased, stem diameter and number of nodes per plant of two cultivars increased. Fresh forage yield of two cultivars linearly increased as phosphate rate was increased from 0 to 20kg/10a. Plant height for each cultivar was positively correlated with fresh forage yield.

관찰학습에 따른 아동의 운동수행에 관한 효과

조동진,한정선 朝鮮大學校 스포츠科學硏究所 2007 스포츠科學硏究 Vol.18 No.-

The present study purposed to examine the effects of children's learning of physical education activities in elementary school according to model type and to provide basic strategic materials for observational learning through presenting demonstrative models in the field of physical education for children. The participants in this study are 40 male sixth graders. They were assigned to the non model group, the skillful model observation group, the learning model observation group or the mixed model observation group at random, and 10 students to each group. The task used in this study was shooting, a basic motion in soccer, and the experiment was conducted at a mini soccer field suitable for children. The shooting motion pattern score was given for dribble shoot, pass short and penalty shoot, and the score ranged from 1 to 5 points. In this study, statistical processing used SPSS (Ver 12.0) for Windows, and AxB two way ANOVA with repeated measures on blocks was performed to see differences between the acquisition and retention stages, among the observation groups, and among the divisions, Statistics related to the research task were analyzed at significance level a=0.05, focused on main effects and interaction effects among the groups and among the blocks, and posttest on the significance of difference among the groups and among the blocks was performed using Tukey's HSD. The results of this study are summarized as follows. First, no statistically significant difference was observed in the score of shooting pattern at the acquisition stage and the retention stage between the control group bservation groups. Second, no statistically significant difference was observed in the score of shooting pattern at the acquisition stage and the retention stage according to model type. Third, in the children s learning of shooting as a physical education activity, the score of shooting pattern at the acquisition stage according to the type of model presented (no model, skillful model, learning model, mixed model) showed the tendency of increasing through the learning compared to that before the learning. That is, statistically significant difference was observed among the division at the acquisition stage but not at the retention stage.

민어(Miichthys miiuy)의 卵發生過程 및 仔稚魚 발육단계에 따른 형태발달

한경호,오성현,황동식,조영현,서대철 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.2

본 연구는 2000년 8월 19일 전라남도 신안군 임자도 연안에서 2각망으로 어획된 민어 어미를 인위적인 방법으로 수정하여 부화 사육하면서 관찰한 卵發生 過程 및 仔稚魚 형태변화는 다음과 같다. 1. 민어의 受精卵은 구형의 분리 부성난으로 알의 크기는 0.96~1.08㎜(평균 1.01㎜, n=50)였고, 크고 작은 油球가 1~5개(0.06~0.34㎜) 존재하였다. 2. 사육수온 25.2~28.4℃에서 受精 후 17시간 10분에는 배체가 卵膜을 뚫고 머리부터 孵化하기 시작하였다. 3. 孵化 직후 仔魚의 전장은 2.36~2.63㎜(평균 2.48㎜, n=10)로 전장의 45.0~47.0%에 달하는 큰 卵黃(장경 1.11~1.20㎜, 단경 0.92~0.96㎜)을 가지고 있으며 筋節은 7~8+ 17~19=24~27개였다. 4. 孵化 후 3일째 仔魚는 전장 3.98~4.32㎜(평균 4.16㎜, n=10)로 입은 열려있었고, 이 시기에 섭이활동이 활발하게 관찰되면서 卵黃이 완전히 흡수되어 後期仔魚期로 이행되었다. 5. 孵化 후 10일째 後期仔魚는 전장이 5.88~6.74㎜(평균 6.30㎜, n=10)로 꼬리의 말단이 굽어지기 시작하는 시기로 黑色素胞가 기저후두골 뒷부분의 체측에서 점모양 및 나뭇가지모양 으로 출현하였다. 6. 孵化 후 31일째 개체의 전장은 9.62~12.76㎜(평균 11.12㎜, n=10)로 모든 지느러미가 정수에 달하였고, 체형이나 반문이 成魚와 닮아 種魚期로 이행하였다. Egg development and morphological change of larvae of the brown croaker, Miichthys miiuy, were investigated after obtaining samples by artificial insemination. The spawning season extends from the middle to the end of August in the seas off Lim-ja Island, Cheollanam-do. The fertilized eggs were transparent, spherical, separate and they floated. Egg diameter ranged 0.96~1.08㎜ with several oil globules of 0.06~0.34㎜. Hatching occurred 17 hours 10 minutes after fertilization in 25.2~28.4℃ water temperature. The newly-hatched larvae were 2.36~2.63㎜(mean:2.48㎜, n=10) in total length(TL), had a large yolk, and 7~8+17~19=24~27 myotomes. Melanophores were distributed on the eye lens. Three days after hatching the larvae had attained 3.98~4.32㎜ in TL(mean:4.16㎜, n=10), the yolk sac was completely absorbed, and they had transformed into the postlarva stage. The larva reached the juvenile stage with all fins formed and with a complete set of fin rays(D.Ⅹ-28~31;A.Ⅱ-7~8;P. 21~23;C. 19~21;V. 25~26) on the 31st day after hatching. The juveniles were 9.62~12.76㎜(mean:11.12㎜, n=10) in total length. At this point the juvenile was similar to the adult in body form and pigmentation pattern.