The miR-145-5p/CD36 pathway mediates PCB2-induced apoptosis in MCF-7 cells

Yuan Yuan,Caihua Xue,Qiang Wu,Mengjie Wang,Jiahua Liu,Longfei Zhang,Qianwen Xing,Jingyan Liang,Hua Wu,Zhi Chen 한국유전학회 2021 Genes & Genomics Vol.43 No.2

Background Procyanidin B2 (PCB2) can increase the levels of anti-infammatory and immune mediators. Objectives However, its molecular mechanism in human breast cancer remains unclear. This study aimed to investigate the antitumor efect of PCB2 on MCF-7 cells and to examine the underlying mechanism. Methods The fow cytometry and EdU incorporation assays were measured the PCB2-induced BMECs. The expression levels of infammatory factors and immune response genes were upregulated in MCF-7 cells, high-throughput sequencing was used to detect diferentially expressed genes in blank and PCB2-treated MCF-7 cells. Results The results showed that PCB2 induced the apoptosis of MCF-7 cells. CD36 profles were afected in MCF-7 cells. Additionally, prediction software identifed a miR-145-5p binding site in the CD36 sequence. Luciferase reporter assays and Western blot analysis were used to verify the regulatory relationships between the diferentially expressed miRNA miR145-5p and CD36. MiR-145-5p and its key target (CD36) constitute a potential miRNA-mRNA regulatory pair. Functional studies in MCF-7 cells revealed that CD36 promotes but miR-145-5p inhibits apoptosis. Conclusion Overall, these data suggest that miR-145-5p inhibits the enhancing efect of PCB2 on CD36 expression by binding CD36 and subsequently regulating apoptosis, the immune response and anti-infammatory pathways. These results provide theoretical and experimental support for the treatment of breast cancer.

Stable Cu-Co/C carbon-based composites for efficiency catalytic degradation of Orange II by PMS: Effect factors, application potential analysis, and mechanism

Xiaodan Huang,Caihua Liu,Zhiyong Zhang,V. Vasanthakumar,Huiying Ai,Lei Xu,Ming-Lai Fu,Baoling Yuan 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-

Carbon-based metallic catalysts (CMCs) have emerged as efficient materials for removing water contaminants,but preparing CMCs with high efficiency and stability remains a great challenge. In this study, weprepared a bimetallic composite of Cu-Co/C catalyst via a gelation and calcination method to degradeOrange II by activating peroxymonosulfate (PMS). The characterization results demonstrated that thespecific surface area and pore size of Cu-Co/C catalysts were 1.675 m2/g and 6.313 nm, respectively,which can provide larger catalytic active sites for boosting PMS activation. Moreover, the effects of differentfactors on Orange II degradation efficiency, cyclic experiments, and possible application in realwater bodies were investigated. The Cu-Co/C catalyst can effectively activate PMS, and 50 mg/L ofOrange II was almost completely removed within 15 min. Additionally, the Cu-Co/C catalyst exhibitedhigh degradation efficiency for other organic dye pollutants. The radical quenching experiments indicatedthat the main active species for Cu-Co/C catalyzed degradation of Orange II were SO4and singlet oxygen(1O2). The catalyst demonstrated good stability and performance in real water bodies. We believe thatthis study will promote the application of carbon-based bimetallic catalysts in the environmental remediationprocess that utilize SO4based advanced oxidation.

Inhibition of MicroRNA-15a/16 Expression Alleviates Neuropathic Pain Development through Upregulation of G Protein-Coupled Receptor Kinase 2

( Tao Li ),( Yingchun Wan ),( Lijuan Sun ),( Shoujun Tao ),( Peng Chen ),( Caihua Liu ),( Ke Wang ),( Changyu Zhou ),( Guoqing Zhao ) 한국응용약물학회 2019 Biomolecules & Therapeutics(구 응용약물학회지) Vol.27 No.4

There is accumulating evidence that microRNAs are emerging as pivotal regulators in the development and progression of neuropathic pain. MicroRNA-15a/16 (miR-15a/16) have been reported to play an important role in various diseases and inflammation response processes. However, whether miR-15a/16 participates in the regulation of neuroinflammation and neuropathic pain development remains unknown. In this study, we established a mouse model of neuropathic pain by chronic constriction injury (CCI) of the sciatic nerves. Our results showed that both miR-15a and miR-16 expression was significantly upregulated in the spinal cord of CCI rats. Downregulation of the expression of miR-15a and miR-16 by intrathecal injection of a specific inhibitor significantly attenuated the mechanical allodynia and thermal hyperalgesia of CCI rats. Furthermore, inhibition of miR-15a and miR-16 downregulated the expression of interleukin-1β and tumor-necrosis factor-αin the spinal cord of CCI rats. Bioinformatic analysis predicted that G protein-coupled receptor kinase 2 (GRK2), an important regulator in neuropathic pain and inflammation, was a potential target gene of miR-15a and miR-16. Inhibition of miR-15a and miR-16 markedly increased the expression of GRK2 while downregulating the activation of p38 mitogen-activated protein kinase and NF-κB in CCI rats. Notably, the silencing of GRK2 significantly reversed the inhibitory effects of miR-15a/16 inhibition in neuropathic pain. In conclusion, our results suggest that inhibition of miR-15a/16 expression alleviates neuropathic pain development by targeting GRK2. These findings provide novel insights into the molecular pathogenesis of neuropathic pain and suggest potential therapeutic targets for preventing neuropathic pain development.

The Mechanism of Rockbolt in the Jointed Rock Under Uniaxial Tension

Shen Zhou,Xiaoyu Ji,Li-Ping Li,Hong-liang Liu,Chun Zhu,Hongyun Fan,Qi Zhang,Caihua Shi,Xutong Zhang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.11

In this research, the rockbolt mechanism of a jointed rock mass under uniaxial tension was systematically revealed with a laboratory test and a numerical simulation. It was found that the rockbolt rock mass experienced five stages under uniaxial tension, the densification stage, elastic stage, plastic deformation stage, progressive debonding stage, and complete debonding stage. The stress-strain curve and ultimate tensile strength of a rockbolt rock mass were analyzed by taking the rockbolt spacing and rockbolt angle as variables. It was found that the improvement effect of the reduction of the rockbolt spacing on the ultimate tensile strength was limited. When the rockbolt spacing was reduced to a certain limit, the stress concentration area between adjacent rockbolts was connected and destroyed, resulting in the increase of the rockbolt rock strength becoming smaller, and even having a downward trend. The increase of the rockbolt angle led to the change of the stress mode and failure mode of the whole structure, and the ultimate tensile strength first increased and then decreased. The optimal rockbolt angle was between 60° and 70°. It is worth noting that there was an obvious mechanical occlusion between the thread on the rockbolt surface and the rock mass, resulting in the multi-stage step-down characteristic of the stress-strain curve in the complete debonding stage. The results of this study can provide a reference for the design and construction of similar projects.

Enhancing the Adsorption Capacity of Hematite by Manganese Doping: Facile Synthesis and its Application in the Removal of Congo Red

Huaifen Su,Xiaoxue Wang,Yuanwei Sun,Dan Xu,Lei Li,Caihua Liu,Suyuan Zeng,Dezhi Sun 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.10

This work aims to provide a facile method for the preparation of Mn-doped hematite microrods and reveals the relationship between Mn doping and the enhanced adsorption properties. The adsorption performance of hematite could be greatly enhanced by Mn doping. The maximal adsorption capacity of the Mn-doped α-Fe2O3 is calculated to be 84.54 mg/g, which exhibits obviously enhanced adsorption capacity as compared to the un-doped α-Fe2O3 (33.02 mg/g). To further understand the adsorption process, the adsorption kinetic as well as thermodynamic behaviors are carefully investigated. The results suggest that the adsorption process of CR onto the adsorbents follows the pseudo-second-order kinetic model. Meanwhile, the adsorption process can be designated to be a Langmuir process. The adsorption capacities of CR vary as a function of Mn contents, which are regarded as the synergistic effect between surface areas and pore sizes.