전통누룩 진균류를 이용한 입국의 제조 및 입국곰팡이의 동정

김재호 ( Jae Ho Kim ),권영희 ( Young Hee Kwon ),이애란 ( Ae Ran Lee ),김혜련 ( Hye Ryun Kim ),안병학 ( Byung Hak Ahn ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.4

다양한 향미를 가진 막걸리의 개발을 위해 전통누룩으로부터 분리한 곰팡이로 입국을 제조한 후 품질특성을 분석하여 입국의 규격에 적합하며 이취가 없고 관능이 우수한 9균주를 입국 제조용 우수균주로 최종 선발하였다. 선발된 균주는 Aspergillus oryzae(C1-5-2-2, C20-7-3, CN1.3.1-4, CN16.19.1-1, N152-1, N220-1), Mycocladus corymbiferus (N162-2), Rhizopus oryzae(N20), Lichtheimia corymbifera (N21)로 동정되었으며, 제조한 입국의 산도는 5.0~6.8, 당화력은 128~241sp이었다. Various koji were prepared by fungi isolated from traditional nuruk and their quality characteristics were investigated. Acidity and saccharification power of their koji were ranged in 5.0~6.8 and 128sp~241sp. Nine fungi which were showed good quality and sensory evaluation were identified by analysis of their nucleotide sequences with PCR-amplified 18S rDNA internal transcribed spacer-1(ITS-1) and ITS-4 genes. Among them, six strains were identified as Aspergillus oryzae and the other strains were identified as Mycocladus corymbiferus, Rhizopus oryzae, Lichtheimia corymbifera.

특발성 과호산구 증후군 환자의 치료 도중 발생한 간혈종 1례

이영태,김동기,박지훈,김윤정,김기향,박보민,권민정,김애란,이원식,주영돈,손창학 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Idiopathic hypereosinophilic syndrome is characterized by marked elevation of eosinophil count ( >1,500/㎣) in peripheral blood without explaining etiology. It shows tissue involvement in many organs such as liver, G-I tract, myocar dium, lung, skin, kidney and nerve. Some case of hypereosinophilic syndrome with hepatic involvement have been reported, but not much. We have recently experienced a case with idiopathic hypereosinophilic syndrome of a 40 year old man who presented with RUQ pain. By abdominal CT, ill- defined subcapsular hematoma was detected. Liver biopsy showed eosinophilic infiltration within the portal tract and sinusoid. Drain procedure and corticosteroid adminiiatbrought marked improvement of clinical manifestation, hematologic abnormality and radiologic abnormality.

중년기 여성의 노후생활 인지수준과 미래 노인복 구매행동

이혜임,장애란 서울保健大學 1995 論文集 Vol.15 No.1

The clothing for the elderly appers to be more crucial because the elderly become more and more important groups among customers as the number of the elderly is ever growing and their interest in the clothing is also growing, so that leads the fashion industries to concentrate the marketing for the elderly. The industries try to investigate the present fashion tates of middle aged-women and the expecting changes of their fashion tastes when they get older. In this study, the relationship between the aging and their psychological depression and the pattern of purchasing clothing was studied for middle aged -women. The random questionnaires were performed with 553 middle aged-women who lived in Seoul. Used major statistical methods were t-test, X^(2)-test, oneway-ANOVA, Scheffe's test, factor analysis, multiple regression analysis, Cronbach's α and path analysis. It was found that (i) the aging factor of middle aged-women psychologically affects on choosing clothing; the recognition by others, the psychological protection, the expression of beauty and social activity are important factors for the middle aged-women to select clothing(among the above four factors, the recognition by others and the psychological protection are more important), and (ii) the needs of purchasing elderly-clothing was somewhat strong. The information sources of reference, such as friends of fashion expertise, are very important to make the women's mind in selecting clothing. The middle aged-women generally purchase clothing on the beauty at department stores. The very important determinant of satisfaction after purchasing clothing is social psychology. First of all, dissatisfaction factors were understanded through the realities of the ready-to-wear, these factor were taken into consideration in the making clothing construction, and in future, the ready-to-wear of middle and old aged-women will be more improved.

광합성 세균 Rhodobacter sphaeroides KS56에 의한 수소 생성

이은숙,권애란 동아시아식생활학회 1997 동아시아식생활학회지 Vol.7 No.3

The optimum temperature and pH for growth and hydrogen evolution of the organism were observed at 30~35℃, and around pH 7.0, respectively. The efficiency of various sugars and organic acids on hydrogen evolution as electron donors by the organism was examined. Among them, higher rates of hydrogen evolution were observed with sugars such as glucose or fructose and organic acids such as malate or pyruvate. From the result, it was evident that Rhodobacter sphaeroides KS56 had a great capacity of utilizing various kinds of reduced carbon compounds as electron doners.

생활치 표백술 후 수종의 자유 산소기 제거제 처리가 복합 레진-법랑질 전단 접착 강도 및 파절 양상에 미치는 영향

임경한,금기연,김애리,장수미 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5

Tooth bleaching has been prevailing recently for its ability to recover the color and shape of natural teeth without reduction of tooth material. However, it has been reported that bleaching procedure adversely affects the adhesive bond strength of composite resin to tooth. At the same time the bond strength was reported to be regained by application of some chemical agents. The purpose of this in vitro study was to investigate the effect of the removal of residual peroxide on the composite- enamel adhesion and also evaluated fracture mode between resin and enamel after bleaching. Sixty extracted human anterior and premolar teeth were divided into 5 groups and bleached by combined technique using office bleaching with 35 % hydrogen peroxide and matrix bleaching with 10% carbamide peroxide for 4 weeks. After bleaching, the labial surfaces of each tooth were treated with catalase, 70% ethyl alcohol, distilled water and filled with composite resin. Shear bond strength was tested and the fractured surfaces were also examined with SEM. Analysis revealed significantly higher bond strength values. (p<0.05) for catalase-treated specimens, but water-treated specimens showed reduction of bond strength, alcohol- treated specimens had medium value between the two groups(p<0.05). The fracture mode was shown that the catalase group and the alchohol group had cohesive failure but the water sprayed group had adhesive failure. It was concluded that the per-oxide residues in tooth after bleaching seems to be removed by gradual diffusion and the free radical oxygen from peroxide prevents polymerization by combining catalyst in the resin monomer. Therefore it may be possible to eliminate the adverse effect on the adhesion of composite resin to enamel after bleaching by using water displacement solution or dentin bonding agent including it for effective removal of residual per-oxide Key words : Vital tooth bleaching, Shear bond strength, Composite resin, Fracture mode catalase, Ethylalcohol, Free oxygen radical, Adhesive failure, Cohesive failure

급성심근경색후 발생한 심실중격결손 2례

김태희,이재호,김애란,허민영,문치숙,정수룡,김대경,김두일,김동수 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Ventricular septal defect remains an infrequent but devastating complication of acute MI. Approximately 1∼3% of cases of acute myocardial infarction are complicated by ventricular septal rupture in the prethrombolytic era and the incidence has decreased to 0.2% with reperfusion therapy. This mechanical complication usually occurs within the first 10 to 14 days when necrotic tissue is most abundant and the collateral coronary circulation is not well developed. This lesion is generally associated with complete coronary obstruction rather than severe stenosis. Rupture of the ventricular septum is a severe mechanical complication of acute myocardial infarction, usually resulting in death unless surgical repair is performed. The bad prognosis of this event within the first 2 weeks indicates the need for early surgical rapair. This complication is more frequent after the first acute myocardial infarction in the elderly and secondary to a transmural myocardial infarction. We report two cases of ventricular septal rupture after acute myocardial infarction.

특수학교 전공과 운영개선 방안

류문화,김종무,유애란,이상훈,장병연 국립특수교육원 2000 연구보고서 Vol.- No.3

특수학교 전공과 운영 개선방안 연구는 현재 특수학교에 설치되어 있는 전공과의 전반적인 현황을 문헌을 분석하여 살펴보고, 설문지조사 및 방문조사를 실시하여 전공과 운영실태와 전공과 설치에 대한 요구사항을 파악하여 전공과 운영의 적절성과 효율성을 높이기 위한 개선방안을 제시하는데 그 목적이 있었다. 본 연구의 주된 내용으로는 문헌을 통해 전공과의 설치현황과 설치근거, 설치배경 및 설치과정, 전공과와 직업교육 및 전환교육과의 관계, 전공과 운영의 문제점 등을 분석하여 기술하였으며, 그 다음 설문지조사 및 방문조사 실시 과정과 실시 결과를 요약 정리하여 기술한 후, 이를 토대로 개선방안을 제시하였다. 본 연구의 조사는 2000년 6월 8일부터 6월 30일까지 실시하였으며, 연구방법은 전공과 설치학교의 운영상의 문제점을 파악하기 위해 전국의 특수학교 전공과 설치학교를 대상으로 운영실태 조사를, 지역을 고려한 전공과 미설치학교를 대상으로 요구조사를 설문지를 통해 실시하였다. 이와 병행하여 전공과 설치학교 중 장애영역과 지역을 고려한 14개교를 방문조사 하였으며, 설문지조사는 우편으로 조사하였고, 방문조사는 본원 연구자 및 공동 연구자가 대상학교를 직접 방문하여 면담을 통해 이루어졌다. 다음으로는 설문지조사 및 방문조사를 실시하여 나타난 운영실태 조사와 요구조사 결과에 의해 도출된 문제점과 이에 대한 개선방안을 알아보고 결론과 제언을 제시하고자 한다. 운영실태 조사 결과 나타난 문제점은 전공과에 대하여 전공과에 재학하고 있는 학생의 학부모와 지역사회의 협조가 잘 이루어지지 않으며, 전공과에 대한 국가나 시·도 교육청의 행·재정적인 지원행정이 미흡하고, 학교에서 선택한 직종과 취업의 연계가 어렵고 지원고용이 실시되지 못하고 있는 것으로 나타났다. 그리고 전공과 지도교사에 대한 자격기준을 마련할 것과 성공적인 전공과 운영을 위해서는 전문요원의 학보와 배치를 위한 국가차원의 기준안 마련이 시급하다고 하였으며, 향후 전공과 설치는 현재 전공과를 운영하는데 미흡하고 어려운 점이 있다 하더라도 장애학생들의 사회로의 전환을 위해서는 전공과를 확대 설치되어야 한다는 의견을 제시하였다. 전공과를 설치하고 있지 않은 특수학교 중 21개교가 전공과 운영 요구조사에 참여하였으며, 설문지에 응답한 학교는 대체적으로 현재 전공과 운영에 대한 상황을 파악하고 있었고, 대다수 학교가 전공과 우녕이 부실하게 운영되고 있는 것으로 인식하고 있었다. 또한 전공과가 원활히 이루어지기 위해서는 행·재정적인 지원이 필요하고 전공과 정체성 확립이 필요하다고 응답하였다. 그리고 다수의 교사들이 전공과의 정체성 확립, 교육과정 개발, 전문교원의 배치, 행·재정적인 지원체계 확립을 통해 장애학생들의 학교에서 직업·사회로의 성공적인 전환을 위한 전공과 설치가 필요하다고 하였다. 방문조사 결과를 요약하면, 모든 전공과 설치학교는 교육과정 운영, 교재선정, 교사 배치, 별도의 지침 등이 없기 때문에 학교마다 다양하게 전공과를 운영하고 있었으며 학교 자체계획으로 전공과 관련 학칙 및 운영규칙을 마련하여 시행하고 있었다. 정신지체학교 전공과는 계속 신설되고 있는 추세이며, 현재의 전공과 형태를 수정하여 보호작업장과 그룹 홈의 형태로 운영하는 것을 바라고 있었고, 청각장애학교 전공과 운영의 가장 큰 문제점으로는 입학할 학생이 없어 전공과 운영이 부실하게 운영되고 있었다. 특히, 시각장애학교의 경우는 전공과 운영에 모든 기대를 걸고 있었으며 단순한 기능의 심화나 고등부 연장을 위한 또 다른 형태의 학제가 아닌 전문의료인을 양성하기 위한 심화과정으로 인식하고 있었다. 이상의 내용을 종합하여 결론적으로 전공과 운영 개선방안을 제시하면 다음과 같다. 첫째, 전공과 정체성 정립의 문제로서 전공과의 역할을 분명하게 해야 한다. 둘째, 전공과를 확대 설치해야 하며 각 학교의 장애영역, 지역 등의 특성에 따른 학교별 전공과 운영 모형 및 지침이 개발되어야 한다. 셋째, 전공과 대상학생들에 대한 선발, 직업평가, 직업훈련, 배치, 추수지도 등에 대한 운영체제를 확립해야 한다. 넷째, 전공과의 교육과정은 학생의 수준과 장애의 특성을 고려한 직업훈련 교육과정으로 편성해야 한다. 다섯째, 장애유형에 따라 전공과 운영을 특성화해야 한다. 여섯째, 시설, 장비, 재료 등에 대한 행·재정적 지원체제를 확립해야 한다. 여덟째, 전공과 담당교사의 전문성이 확보해야 한다. 아홉째, 효율적인 전공과 운영을 위해서는 관련부처들(교육부, 노동부, 보건복지부 등)이 상호 협조하는 지원체제가 확립되어야 한다. In our country, professional skill training has been administrated as educational curriculum above one year after graduating high school for the students with disabilities in special school since 1993. This is defined as Specialized Vocational Program(SVP) in this study. This study investigated the actual management condition and management needs assessment of the SVP in order to improve the quality of vocational education through suggesting the way of improvement for appropriateness and effectiveness of menagement. This study was conducted by a review of the literature about the SVP through a survey and interview in the field of special schools. The major content of this study describes the actual condition, basis, background, and progress of the SVP establishment, the relationship among vocational education, the SVP, and transition education, and the problem of management of the SVP. For this study, a mail survey and interview were used. These were conducted from June 8 to June 30 2000. The mail survey on the actual management condition of the SVP was conducted in twenty-five special schools (except one special school, Sunhee School) in the nation. An interview on the actual management condition of the SVP was conducted by researchers in fourteen special schools which were selected based on disability categories and regions. A mail survey on management needs assessment of the SVP was conducted in twenty special schools which do not have the SVP. Descriptive statistical methods were used to analyze the survey data. Following are the results of the study from the survey and interview in this research, discussion of results, and recommendations to improve the management of the SVP. 1. Results of the survey on actual management condition of special school with SVP. 1) Poor Cooperation between parents and community; 2) Poor vacational curriculum and field training; 3) Poor implementation in supported employment; 4) National level qualification reference for professionals and teachers; 5) Poor administrative and financial support from the central and local educational administration for SVP. 2. Results fo the survey on management needs assessment needs assessment of special school without SVP. 1) The actual management condition of SVP is well realized(75%); 2) SVP is poorly implemented in the schools(86%); 3) Appropriate vocational curriculum and field training needs to be developed; 4) Professional personnel needs to be placed in schools; 5) Administrative and financial support from the central and local government for SVP need to be expanded; 6) The identity of SVP needs to be more strongly established. 3. Results of the interview on actual management condition of special school with SVP. 1) There is no national standard for curriculum, textbook selection, and teacher placement on SVP. Thus, each special school develops its own curriculum and administration regulation; 2) The SVP in many schools for mental retardation is being increased. People in these school want a sheltered workshop and a group home program added to the SVP; 3) The SVP in the schools for hearing impairment does not attract students; 4) The curriculum does not fully reflect the student's vocational aptitude, ability, and preference; 5) Cooperation among the educational departments (ministry of education, ministry of labor, and ministry of health and welfare) in the field is not set. Conclusion are as follows. 1) The identity(concept, role, and, purpose) of SVP needs to be clarified. 2) The SVP needs to be installed in more special schools nationwide, and improved according to the local characteristics of the schools. 3) The administrative system of SVP needs to be improved and specialized : Student selection, vocational assesment, training, placement, and follow-up, and category of disability. 4) The curriculum of SVP should be developed according to the students' characteristics, ability, and interest. 5) Financial and administrative support need to be increased for facilities equipment, and materials. 6) Professionalism needs to be guarnateed. 7) Related government departments (ministry of education, ministry of labor, and ministry of health and welfare) need to cooperate more actively for the effective operation of the SVP.

베체트병 환자에서 Azathioprine으로 유발된 급성 췌장염 1례

김기향,권민정,김동기,김애란,김윤정,박지훈,이영태,박보민,김동욱 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Although many drug are thought capable of initiating acute pancreatitis, following azathioprine administration represents the 5% incidence by far the highest recorded incidence to date. We report a 35-year-old male who developed acute pancreatitis in the course of treatment with azathioprine for Behcet's disease. He was admitted due to abdominal pain, diarrhea, and fever for 10 days. Serum amylase and lipase levels were 510U/L and 3702U/L respectively. The abdominal CT scan revealed diffuse enlargement of the pancreas. There was no history of alcohol use or gall bladder disease. We considered drugs, especially azathioprine, as the cause of acute pancreatitis. We diagnosed it as acute pancreatitis. The patient was treated conservatively with hydration and analgesics. The symptom subsided within four days. Five days later, azathioprine was resumed. And the patient complained of abdominal pain. The elevated levels of serum amylase and lipase confirmed the recurrence of pancreatitis. His symptom subsided when azathioprine intake the stopped, and the serum amylase and lipase levels returned to normal level within five days.

Increased c-Fos Expression Contributes to Cell Survival Enhanced by Stromal Cell Derived Factor-1/CXCL12 in Synergy with other Cytokines in Mo7e Cells

( Ae Ran Lee ),( Jeong A Park ),( Young Eun Kim ),( Hyung Joo Kwon ),( Young Hee Lee ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.14

Stromal cell-derived factor-1 (SDF-1/CXCL12) enhances the survival of hematopoietic stem cells, progenitor cells, and a human progenitor cell line MO7e, especially in synergy with other cytokines. Previously, we reported that c-Fos was synergistically induced by combined stimulation with SDF-1/CXCL12 plus other cytokines. Here, we aimed to evaluate that induced c-Fos expression is required for the survival enhancement in MO7e cells. First by using RNase protection assay, we confirmed that synergistic induction of c-Fos was unique among several stress-related genes. Since c-Fos is known as one of the components of transcription factor AP-1, we performed electrophoretic mobility shift assay. The DNA binding activity of AP-1 was also synergistically enhanced by combined stimulation, and c-Fos was identified in the DNA bound complex. To further evaluate the significance of c-Fos expression for survival of MO7e cells, we employed cell permeable c-Fos dominant negative mutant form (A-Fos-MTS) and protein transduction procedure. Inhibition of c-Fos by treatment with A-Fos-MTS significantly reduced survival of MO7e cells in the presence of SDF-1/CXCL12 plus low concentration of GM-CSF. Therefore, we suggest that induction of c-Fos and enhanced AP-1 activity contribute to survival effect of cytokines in MO7e cells.

Food Science/Microbiology : Characterization of a 27 kDa Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH86-1 Isolated from Cheonggukjang

Ae Ran Lee,Gyoung Min Kim,Jae Yong Park,Hyeon Deok Jo,Jae Ho Cha,Young Sun Song,Ji Yeon Chun,Jeong Hwan Kim 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1

Bacillus amyloliquefaciens CH86-1 isolated from cheonggukjang was found to have strong fibrinolytic activity when grown on Luria-Bertani medium, and this activity increased sharply when the cells entered the stationary phase. The major fibrinolytic enzyme