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Abdul Nasir Sofian,Md. Habibur Rahman,Yun Ju Jeong,Eun-Sook Jeong,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2021 한국물학회지 Vol.9 No.1
Hydrogen mineral disinfectant farming (HMDF) applies hydrogen mineral disinfectant water which is containing hypochlorous acid component in the production quality and high yield agricultural produce. Hydrogen mineral disinfectant (HMD) water has been found to have antimicrobial effects and is used in washing eggs, processing meat and the control of disease in livestock. In poultry, it increases body weight of livestock and also reduces smell in the farm. Endotoxin or lipopolysaccharide is part of outer membrane architecture of gram-negative bacteria, and a common food contaminant, which poses great health risk to humans, characterized by sepsis, shock or even death. Endotoxin detection and removal is an important part of the industrial food, pharmaceutical and medical equipment production process. Our study aims to compare the endotoxin levels of eggs produced by HMDF and eggs on the market. The limulus amoebocyte lysate test was used to determine the level of endotoxin in whole liquid eggs from HMDF (n = 12) and eggs from the market (n = 12), randomly sampled. Analysis of variance followed by multiple comparison test was used to analyze the data. As a result, The endotoxin level was significantly low in the experimental eggs (0.25 EU/mL) compared to the contol (10 EU/mL) (p >0.005). This result suggests that the application of HMDF in egg production could reduce risk associated with endotoxin-induced health problems.
Abdul Rahman Abdul Rahim,Iswarya,Khairiraihanna Johari,Nasir Shehzad,Norasikin Saman,Hanapi Mat 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.4
Desiccated coconut waste (DCW) is an agricultural waste that originates from the coconut milk processing industry. In this study, it was utilized for the removal of copper (Cu(II)) and nickel (Ni(II)) via adsorption process. The physicochemical characterization of the DCW adsorbent shows that the adsorbent have a surface area of 6.63 ㎡/g, have high elemental carbon content and existences of important functional groups on its surface. The adsorptive capability of DCW adsorbent in removing the heavy metal were conducted in batch studies. DCW adsorbent performed highest Ni(II) and Cu(II) adsorption capacity at pH 6, where equilibrium is achieved at 450 minutes. The kinetic analysis showed the adsorption agreed with pseudo-second order kinetic model, indicating the Cu(II) and Ni(II) adsorption were a chemical adsorption, limited by the film diffusion. The DCW adsorbent still retained its effective adsorption capacity after 2 adsorption-desorption cycles, which is one of the excellent criteria of a good adsorbent for an adsorption process.
( Nasir Abdul ),유태현 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
The production of biodiesel has become an important strategy to address the fading petroleum reservoirs and environmental issues. Recently biological processes to produce high-value products from glycerol have drawn much attention. The first step of such processes is the dehydration from glycerol to form 3-hydroxypropanal (3-HPA), which can be catalyzed by glycerol dehydratase (GDHt). During the purification of KpGDHt, β subunit was highly prone to dissociate from the functional (αβγ)2 complex. This has been circumvented through the addition of a linker to fuse the α and β subunits to simplify the purification. Interestingly, the catalytic activity of the linked enzyme was improved than the unfused wild type. The structure, dynamics and kinetics advantages of this arrangement in fused proteins are not known. Therefore, it is of interest to probe into the structure and dynamics properties of the fused and unfused structures of KpGDHt using molecular dynamics simulation.
Rational Engineering of Glycerol Dehydratase for production of 3-hydroxypropionic acid from glycerol
( Nasir Abdul ),유태현 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
The production of biodiesel has become an important strategy to address the fading petroleum reservoirs and environmental issues. Recently biological processes to produce high-value products from glycerol have drawn much attention. Glycerol dehydratase (GdHt)-dimer of an αβγ-heterotrimer (α2β2γ2), involved in the dehydration of glycerol to form 3-hydroxypropanal (3-HPA), and the intermediate can be converted into 1,3-propandiol or 3-hydroxypropionic acid. The dissociation of β subunit in purification has been circumvented through the linking of α and β subunits which interestingly increase the catalytic activity of the linked enzyme. Therefore, it is of interest to probe into the structure and dynamics properties of the linked and unlinked structures of KpGDHt using molecular dynamics simulation. The information about GDHt and the engineering method will be a valuable source to engineer GDHt to convert glycerol into valuable products.