만성 뇌경막하 혈종 외막의 병리조직소견과 외막 투과성에 대한 연구

박윤관,이기찬,이훈갑 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.4

Permeability of membrane of chronic SDH is a unique characteristic. And various types of attenuation of chronic subdural hematoma(SDH) appearing on computerized tomography(CT) scans are related to the changes occurring as the result of recurrent bleeding. The authors have investigated the membrane permeability of SDH according to the histological changes of the outer membrane of chronic SDH. A consecutive seires of 19 adult patients with chronic subdural hematoma has been studied in respect to the post-operative subdural drainage after burr-hole craniostomy and closed system drainage. The subdural drain was kept for 5 days. The amount and the pattern of daily drainage were correlated with histological findings of the outer membrane. The outer membrane of chronic SDH was very rich in blood vessels and contains giant capillaries. One electron microscopic finding of giant capillaries was the frequent formation of gap junctions between adjacent endothelial cells. Numerous red or white blood cells was spilling into the interstitial space of the outer membrane. In positive cases to both membrane hemorrhage and surface clot, total amount of drainage was significantly different from the cases without these findings(p=0.0263). In cases without membrane hemorrhage, the presence of migrating inflammatory cell was well correlated with total drainage amount(p=0.028). From these findings it is suggested that endothelial gap junction of macrocapillaies might play an important role in permeability of the outer membrane. But the recent episode of bleeding could act as a barrier and decrease the permeability actually. After resolution of fibrin clot infiltrated inflammatory cells which contribute to fibrinolysis would decrease and then the permeability would increase.

Functional properties of the major outer membrane protein in Stenotrophomonas maltophilia

Yih-Yuan Chen,Han-Chiang Wu,Juey-Wen Lin,Shu-Fen Weng 한국미생물학회 2015 The journal of microbiology Vol.53 No.8

Stenotrophomonas maltophilia is an opportunistic pathogen that is closely associated with high morbidity and mortality in debilitated and immunocompromised individuals. Therefore, to investigate the pathogenesis mechanism is urgently required. However, there are very few studies to evaluate the functional properties of outer membrane protein, which may contribute to the pathogenesis in S. maltophilia. In this study, three abundant proteins in the outer membrane fraction of S. maltophilia were identified by liquid chromatography- tandem mass spectrometry as OmpW1, MopB, and a hypothetical protein. MopB, a member of the OmpA family, was firstly chosen for functional investigation in this study because many OmpA-family proteins are known to be involved in pathogenesis and offer potential as vaccines. Membrane fractionation analyses demonstrated that MopB was indeed the most abundant outer membrane protein (OMP) in S. maltophilia. For functional studies, the mopB mutant of S. maltophilia (SmMopB) was constructed by insertional mutation. MopB deficiency resulted in a change in the protein composition of OMPs and altered the architecture of the outer membrane. The SmMopB strain exhibited reduced cytotoxicity toward L929 fibroblasts and was more sensitive to numerous stresses, including human serum, sodium dodecyl sulfate, and hydrogen peroxide compared with wildtype S. maltophilia. These results suggest that MopB may be a good candidate for the design of vaccines or anti-MopB drugs for controlling serious nosocomial infections of multidrug- resistant S. maltophilia, especially in immunosuppressed patients.

Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1

윤성호,이상엽,최치원,이하영,노현주,전상미,권용민,권개경,김상진,김건화,김승일 한국미생물학회 2017 The journal of microbiology Vol.55 No.1

Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several polycyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo[a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25–70 nm. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N. pentaromativorans are the major protein components of OMVNovo. Comparative proteomic analysis with the membrane-associated protein fraction and correlation analysis demonstrated that the outer membrane proteins of OMVNovo originated from the membrane- associated protein fraction. To the best of our knowledge, this study is the first to characterize OMV purified from halophilic marine bacteria.

Construction of Antigen 43 System for Escherichia coli-derived Outer Membrane Particles Displaying Recombinant Antigen

Hyo-won YOON,Gna AHN,Yang-Hoon KIM,Ji-Young AHN 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Antigen 43(Ag43), an autotransporter protein, consists of the N-terminal signal peptide sequence to move into inner membrane, the C-terminal β-barrel domain forming a pore in the outer membrane and the passenger domain exposed to bacterial surface. By replacing the passenger domain sequence with desired recombinant antigen, the Ag 43 system can be used to the bacterial surface display. We confirmed constructs of the β-barrel domain by deletion of the N-terminal sequence and evaluated to expose protein to the surface. It was performed by exposing eGFP to the surface, and the Ag43_700 was the most efficient construct through the flow cytometry analysis and extraction of outer membrane. This exposed platform enables the application of antigen display on outer membrane particles.

Chlorhexidine digluconate가 세균외막과 인공세포막의 회전운동에 미치는 비대칭적 영향

김태영,차성권,엄승일,배문경,배수경,주지민,윤일희,김다솔,장혜옥 대한구강악안면병리학회 2021 대한구강악안면병리학회지 Vol.45 No.3

Chlorhexidine is a cationic bisbiguanide with broad antibacterial activity, and diverse activity encompassing gram-positive and gram-negative bacteria, yeasts, dermatophytes, and some lipophilic viruses. Chlorhexidine alters the permeability of the bacterial membrane. They also neutralize periodontal pathogens such as Streptococcus aureus, Porphyromonas gingivalis, and Prevotella intermedia. Porphyromonas gingivalis were cultured to isolate the Porphyromonas gingivalis outer membranes (OPG). Also, OPG were used to prepare large unilamellar liposomes with total lipids (OPGTL) extracted and prepared with the phospholipids mixture (PL). The effect of chlorhexidine digluconate on cell membrane kinetic changes was investigated using fluorescence polarization of n-(9-anthroyloxy) stearic acid with different rotational mobility depending on the probe substitution position (n) in the membrane phospholipid aliphatic chain. Chlorhexidine digluconate increased the hydrocarbon rotational mobility interior of the OPG, OPGTL and PL, but native and model membranes interfacial mobility were decreased. The sensitivity to increasing chlorhexidine digluconate effect on rotational mobility was proportional to the depth of probe position in descending order of 16-AP, 12-AS, 6-AS and 9-AS. Chlorhexidine digluconate disordering or ordering the effects on the membrane lipids may cause its bacteriostatic and bacteriocidal actions.

Postoperative changes in the retinal thickness and volume after vitrectomy for epiretinal membrane and internal limiting membrane peeling

Won, Jae Yon,Kim, Mirinae,Park, Young-Hoon Wolters Kluwer Health 2017 Medicine Vol.96 No.19

<P><B>Abstract</B></P><P>The aim of the study was to investigate the thickness and volume profiles of each retinal layer in postoperative patients with epiretinal membranes.</P><P>Twenty-four patients who underwent pars plana vitrectomy with internal limiting membrane (ILM) peeling for epiretinal membrane were included. The best corrected visual acuity, thickness, and volume were recorded from the medical records through a retrospective review. Spectral domain optical coherence tomography was used to measure the average thickness and volume of each retinal layer before surgery and 6 months postoperatively.</P><P>All 24 patients were monitored for 60 months after surgery. In all Early Treatment Diabetic Retinopathy Study (ETDRS) subfields, the thickness and volume of the retinal nerve fiber layer and the inner retinal layer decreased significantly. In contrast, the thickness and volume of the ganglion cell layer, inner nuclear layer, inner plexiform layer, and outer plexiform layer only decreased in some ETDRS subfields. Finally, there was no significant change in the thickness or volume of the outer nuclear layer (ONL), retinal pigment epithelium (RPE), and photoreceptor layers in all ETDRS subfields.</P><P>The thickness and volume of the inner retina layer decreased significantly after pars plana vitrectomy using ILM peeling. However, there was no significant change in the thickness and volume of the outer retinal layers (ONL, RPE, and photoreceptor) after surgery.</P>

The Transmembrane Domain of AtTco64 and Its C-terminal Lysine-rich Flanking Region Are Targeting Signals to the Chloroplast Outer Envelope Membrane

이용직,황인환,Eun Ju Sohn,Kwang Hee Lee,이동욱 한국분자세포생물학회 2004 Molecules and cells Vol.17 No.2

The targeting mechanism of chloroplast outer enve-lope membrane proteins remains largely unknown. We investigated the targeting of AtToc64. In protoplasts, the transmembrane domain (TMD) and its C-terminal lysine-rich flanking region (LFR) were both necessary and sufficient for targeting to the outer envelope membrane. The lysine residues of the flanking region were critical; without the LFR, the TMD was targeted to the ER or the plasma membrane. In addition, the types of amino acid residues of the TMD, but not the amino acid sequence per se, is a signal for targeting to the chloroplast envelope membrane. TMDs containing phenylalanines were not targeted to the chloroplast in vivo. Based on these results, we propose that the chloroplast targeting signal of AtToc64 comprises two different components: 1) the LFR, which is a signal for evading SRP-mediated co-translational translocation and 2) the hydrophobic amino acid side chains of the TMD, whose size functions as a signal for a cytosolic factor that mediates transport to the chloroplast

High-performance nanofiltration of outer-selective thin-film composite hollow-fiber membranes via continuous interfacial polymerization

최욱,백동현,박철호 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.103 No.-

Outer-selective thin film composite (TFC) hollow fiber membranes have the advantages of fiber blockagewith less pressure drop and high surface area module. This work highlights novel strategies to designcontinuous interfacial polymerization that can allow mass production, performance controllability, andsmall margin of error in performance. Processing parameters were symmetrically optimized to maximizehigh water flux and salt rejection using poly(piperazine-amide) TFC membranes. For further improvingthe membrane performance, polyvinyl alcohol was incorporated for an interlayer, thereby having thewater flux of 48 LMH/bar and the salt rejection (Na2SO4) of 99.6%. The system can be used in a varietyof applications such as reverse osmosis, forward osmosis, or gas separations by simply replacing themonomers.

Salmonella Gallinarum 세포외막단백질의 프로테옴 분석 및 닭에서의 방어능 효과

선지선,조영재,장주현,강정무,한장혁,한태욱,Sun, Jisun,Cho, Youngjae,Jang, Joo-Hyun,Kang, Zheng-Wu,Han, Jang-Hyuk,Hahn, Tae-Wook 한국축산식품학회 2013 한국축산식품학회지 Vol.33 No.2

Salmonella Gallinarum (SG) is known as an important pathogen that causes fowl typhoid in chickens. To investigate SG outer-membrane proteins (OMPs) as a vaccine candidate, we used proteomic mapping and database analysis techniques with extracted OMPs. Also, extracted OMPs were evaluated in several aspects to their safety, immune response in their host and protective effects. Our research has established a proteomic map and database of immunogenic SG-OMPs used as inactive vaccine against salmonellosis in chickens. A total of 22 spots were detected by 2-dimensional gel electrophoresis and immunogenic protein analysis. Eight spots were identified by Matrix-Assisted Laser Desorption/Ionization-Time of Flight-Mass spectrometry (MALDI-TOF-MS) and peptide mass fingerprinting (PMF) and categorized into four different types of proteins. Among these proteins, OmpA is considered to be an immunogenic protein and involved in the hosts' immune system. To estimate the minimum safety dose in chickens, 35 brown layers were immunized with various concentrations of OMPs, respectively. Consequently, all chickens immunized with more than a $50{\mu}g$ dose were protected against challenges. Moreover, intramuscular administration of OMPs to chickens was more effective compared to subcutaneous administration. These results suggest that the adjuvanted SG-OMP vaccine not only induces both the humoral and cellular immune response in the host but also highly protects the hosts' exposed to virulent SG with $50{\mu}g$ OMPs extracted by our method.

Salmonella typhimurium 외막 단백질 OmpW의 발현조절 및 기능에 관한 연구

유아영(Ah Young Yoo),유종언(Jong Earn Yu),양지선(Jiseon Yang),김영희(Young Hee Kim),백창호(Chang Ho Baek),오정일(Jeong-Il Oh),강호영(Ho Young Kang) 한국생명과학회 2008 생명과학회지 Vol.18 No.11

Salmoenella를 포함한 그람 음성 세균의 외막 단백질들은 외부 환경 조건에 따라 그 발현에 변화가 생기는 경우가 많으며, 이렇게 발현된 외막 단백질들은 수송에 관여하는 통로, 외부 물질의 인지, 병원체의 부착인자 등 다양한 기능을 가진다. 본 연구에서는 이러한 외막 단백질 중 소수성 porin으로 알려진 OmpW 단백질에 주목하였다. ompW 유전자가 결손된 돌연변이주를 구축하여 CK10으로 명명하였으며, 야생주와 그 표현형적 특징을 비교한 결과 SDS에 대한 내성이 다소 증가함을 확인할 수 있었다. OmpW 특이적인 다클론성 항체를 조제하여 OmpW 단백질의 발현연구에 사용하였다. NaCl의 농도가 높아질수록 OmpW 단백질의 발현이 감소하였으며, OmpW의 발현이 최대인 조건은 NaCl이 배지에 첨가되지 않았을 경우이다. 따라서 OmpW는 Salmonella 균이 삼투환경에 대응하는데 관여할 것으로 예상되어 일차적으로 균의 생육을 조사한 결과, 다양한 삼투환경 하에서 Salmonella의 생육에는 OmpW 단백질의 결손이 큰 영향을 미치지는 않는 것을 확인하였다. 삼투환경 변화에 따른 OmpW 발현의 변화가 지니는 생물학적 의미는 앞으로 연구해야 할 숙제로 남는다. Outer membrane proteins (OMPs) expressed in the Gram negative bacteria such as Salmonella play multiple functions including material transports, adhesive factors and reception of external signals. This study has been focused on an OmpW protein known as a protein required to form a hydrophobic porin in outer membrane. We have constructed a S. typhimurium CK10 mutant deleting an ompW gene on chromosome. The CK10 strain was more tolerant to SDS than the wild-type strain did. As increase of salt concentration in the culture media, significantly decreased amount of OmpW protein in cells were detected. The maximum OmpW protein was expressed in the absence of salt supplement. However, the growth of CK10 strain was indistinguishable compared to that of the wild-type strain at the variable osmotic conditions. The biological role of differential OmpW expression in response to osmotic conditions remains to be investigated.