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RISS 인기검색어
- 검색키워드
- 발행기관 : R. Wegmann (검색결과 4 건)
- 무료
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Im, W,Chung, J-Y,Kim, S-H,Kim, M R. Wegmann 2011 Cellular and molecular biology Vol.57 No.suppl
<P>Human adipose-derived stem cells (hASCs) are a feasible source of stem cells for use in clinical applications. hASCs are typically cultured in medium containing fetal bovine serum (FBS); however, use of FBS is not recommended due to issues of clinical safety with regard to infections or immune response. Replacement of FBS with autologous human serum (autoHS) can eliminate these problems; however, their maintainability as potent ASCs in autoHS needs to be confirmed. Thus, we conducted an investigation of characterizations and functions of hASCs grown in medium containing autoHS compared to FBS. Cell counting and the WST-8 assay were used in assessment of the proliferation rate. In hASC cultured with culture medium plus autoHS or FBS, cell phenotypes were characterized by flow cytometry (CD13, CD29, CD31, CD34, and CD44) and expression of BDNF, HGF, IGF, LIF, NGF, and VEGF was determined by RT?CR. Adipogenic differentiation was confirmed by oil red O stain. hASC showed greater expansion in AutoHS than in FBS. Cell surface markers of hASCs grown in autoHS (autoHS-hASCs) were similar to markers of those grown in FBS (FBS-hASCs). AutoHS-hASCs also expressed multiple growth factors as well as FBS-hASCs. In addition, autoHS was effective in growth of hASCs as well as FBS and autoHS-hASCs retained their ability for adipogenic differentiation. In summary, autoHS-hASCs have multiple growth factor expressions with the same cell surface markers as FBS?ASCs in vitro. Our results suggest that autoHS can provide sufficient ex vivo expansion of hASCs.</P>
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Kirby, K A,Singh, K,Michailidis, E,Marchand, B,Kodama, E N,Ashida, N,Mitsuya, H,Parniak, M A,Sarafianos, S G R. Wegmann 2011 Cellular and molecular biology Vol.57 No.1
<P>4'—Ethynyl—2—fluoro—2'—deoxyadenosine (EFdA) is the most potent inhibitor of HIV reverse transcriptase (RT). We have recently named EFdA a Translocation Defective RT Inhibitor (TDRTI) because after its incorporation in the nucleic acid it blocks DNA polymerization, primarily by preventing translocation of RT on the template/primer that has EFdA at the 3'—primer end (T/PEFdA). The sugar ring conformation of EFdA may also influence RT inhibition by a) affecting the binding of EFdA triphosphate (EFdATP) at the RT active site and/or b) by preventing proper positioning of the 3'—OH of EFdA in T/PEFdA that is required for efficient DNA synthesis. Specifically, the North (C2'—exo/C3'—endo), but not the South (C2'—endo/C3'—exo) nucleotide sugar ring conformation is required for efficient binding at the primer—binding and polymerase active sites of RT. In this study we use nuclear magnetic resonance (NMR) spectroscopy experiments to determine the sugar ring conformation of EFdA. We find that unlike adenosine nucleosides unsubstituted at the 4'—position, the sugar ring of EFdA is primarily in the North conformation. This difference in sugar ring puckering likely contributes to the more efficient incorporation of EFdATP by RT than dATP. In addition, it suggests that the 3'—OH of EFdA in T/PEFdA is not likely to prevent incorporation of additional nucleotides and thus it does not contribute to the mechanism of RT inhibition. This study provides the first insights into how structural attributes of EFdA affect its antiviral potency through interactions with its RT target.</P>
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Asarone inhibits adipogenesis and stimulates lipolysis in 3T3-L1 adipocytes.
Lee, S H,Kim, K-Y,Ryu, S Y,Yoon, Y,Hahm, D-H,Kang, S A,Cho, S H,Lim, J-S,Moon, E-Y,Yoon, S R,Lee, H G,Yoon, D Y,Yang, Y R. Wegmann 2010 Cellular and molecular biology Vol.56 No.suppl
<P>Asarone is a molecule found in certain plants such as Acorus calamus, the root of which is used in traditional medicine to treat diabetes. We determined the molecular mechanism underlying the anti-diabetic activity of asarone. Treatment of asarone significantly inhibited the differentiation of 3T3-L1 preadipocytes through suppression of expression of the transcription factors, CCAAT/enhancer binding protein-alpha and peroxisome proliferator activated receptor-gamma, which activate adipogenesis. Intracellular triglyceride levels were reduced by asarone in a dose-dependent manner and asarone treatment stimulated the phosphorylation of hormone-sensitive lipase. Together, the present findings indicate that asarone inhibits adipogenesis by down-regulation of PPARgamma and C/EBPalpha and reduces lipid accumulation by stimulation of lipolysis through an increase in hormone-sensitive lipase activity.</P>
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VDUP1 potentiates Ras-mediated angiogenesis via ROS production in endothelial cells.
Piao, Z H,Yoon, S R,Kim, M S,Jeon, J H,Lee, S H,Kim, T D,Lee, H G,Bae, K H,Min, J K,Chung, S J,Kim, M,Cho, Y S,Oh, D B,Park, S Y,Chung, J W,Choi, I R. Wegmann 2009 Cellular and molecular biology Vol.55 No.suppl
<P>Vitamin D3 up-regulated protein 1 (VDUP1) is a tumor suppressor of which expression is reduced in a variety of cancer cells, and enforced expression inhibits the tumor cell proliferation. It inhibits the activity of thioredoxin, thus contributing cellular ROS generation. Since ROS is a critical factor for angiogenesis, we investigated the role of VDUP1 in angiogenesis and endothelial proliferation. The expression of VDUP1 was upregulated by overexpression of an oncogene, Ras. Enforced expression of VDUP1 increases ROS production and proliferation of Ras-overexpressing endothelial cells. Overexpression of VDUP1 increases the resistance to the anchorage-dependent cell death and tube formation of the Ras-overexpressing endothelial cell. In addition, the removal of ROS by ROS scavenger attenuates the effect of VDUP1 on tube formation. These results suggest that VDUP1 is involved in Ras-mediated angiogenesis via ROS generation in endothelial cells.</P>
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