http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Bhuiyan, Mohammed Shafi Ullah,Min, Sung Ran,Jeong, Won Joong,Sultana, Sayeda,Choi, Kwan Sam,Lim, Yong Pyo,Song, Won Yong,Lee, Youngsook,Liu, Jang R. Japanese Society for Plant Cell and Molecular Biol 2011 Plant biotechnology Vol.28 No.1
<P>An efficient <I>Agrobacterium</I>-mediated genetic transformation method was established for <I>Brassica juncea</I> by investigating several factors responsible for successful gene transfer. Four-day-old cotyledon explants from <I>in vitro</I> grown seedlings were co-cultivated with <I>Agrobacterium</I> strain GV3101 harboring the binary vector EnPCAMBIA1302-YCF1, which contained the hygromycin phosphotransferase (<I>HPT</I>) gene as a selectable marker and the yeast cadmium factor 1 (<I>YCF1</I>) gene. Two days co-cultivation period on shoot induction medium (MS medium supplemented with 0.1 mg l<SUP>−1</SUP> α-naphthaleneacetic acid, 1.0 mg l<SUP>−1</SUP> 6-benzyladenine, and 2.0 mg l<SUP>−1</SUP> silver nitrate) containing 20 mg l<SUP>−1</SUP> acetosyringone and five days delaying exposure of explants to selective agent enhanced transformation efficiency significantly. A three-step selection strategy was developed to select hygromycin resistant shoots. Hygromycin-resistant shoots were subsequently rooted on root induction medium. Rooted plantlets were transferred to pot-soil, hardened, and grown in a greenhouse until maturity. Using the optimized transformation procedure, transformation efficiency reached at 16.2% in this study. Southern blot analysis was performed to confirm that transgenes (<I>HPT</I> and <I>YCF1</I>) were stably integrated into the plant genome. All transgenic plants showed single-copy of transgene integration in the host genome. Segregation analysis of T<SUB>1</SUB> progeny showed that the transgenes were stably integrated and transmitted to the progeny in a Mendelian fashion.</P>
<i>Agrobacterium</i>-mediated genetic transformation of radish (<i>Raphanus sativus</i> L.)
Cho, Mi Ae,Min, Sung Ran,Ko, Suk Min,Liu, Jang Ryol,Choi, Pil Son Japanese Society for Plant Cell and Molecular Biol 2008 Plant biotechnology Vol.25 No.2
<P>In order to generate transgenic radish (<I>Raphanus sativus</I> L., cv. Jin Ju Dae Pyong), hypocotyl explants were cultured on Murashige and Skoog medium containing 4 mg l<SUP>−1</SUP> AgNO<SUB>3</SUB>, 5 mg l<SUP>−1</SUP> acetosyringone, 4 mg l<SUP>−1</SUP> 6-benzyladenine, and 3 mg l<SUP>−1</SUP> α-naphthaleneacetic acid in addition to either 10 mg l<SUP>−1</SUP> hygromycin or 100 mg l<SUP>−1</SUP> paromomycin after co-cultivation with disarmed <I>Agrobacterium tumefaciens</I> harboring a plant expression binary vector. Explants co-cultivated with <I>A. tumefaciens</I> GV3101 harboring pCAMBIA1301 and <I>A. tumefaciens</I> EHA101 harboring pPTN290 produced putative transgenic adventitious shoots at frequencies of 0.26% and 0.18%, respectively. Northern blot analysis revealed the <I>gus</I> gene transcript was detected in 8 regenerated plants which confirmed their genetic transformation. The transgenic plants were grown to maturity after vernalization in a greenhouse and appeared morphologically normal. Progeny analysis of independent transgenic plants demonstrated that the <I>gus</I> gene was transmitted in a Mendelian pattern in 3 lines, indicating a single copied gene was incorporated into the genome.</P>