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Christophe, Thierry,Ewann, Fanny,Jeon, Hee Kyoung,Cechetto, Jonathan,Brodin, Priscille Future Science 2010 Future medicinal chemistry Vol.2 No.8
<P>Macrophages are reservoirs for replicating mycobacterium during tuberculosis (TB) infections. In this study, small molecules to be developed as anti-tubercular treatments were investigated for their ability to kill intracellular bacteria in in vitro macrophage models. High-content imaging technologies offer a high-throughput method to quantify a drug's ability to inhibit Mycobacterium tuberculosis intracellular invasion and multiplication in host cells. Dedicated image analysis enables the automated quantification of infected macrophages, and compounds that inhibit mycobacteria proliferation can be tested using this method. Furthermore, the implementation of the assay in 384-well microtiter plates combined with automated image acquisition and analysis allows large-scale screening of compound libraries in M. tuberculosis-infected macrophages. Here we describe a high-throughput and high-content workflow and detail its utility for the development of new TB drugs.</P>
Thangapandian, Sundarapandian,John, Shalini,Son, Minky,Arulalapperumal, Venkatesh,Lee, Keun Woo Future Science 2013 Future medicinal chemistry Vol.5 No.1
<P>Human LTA4H catalyzes the conversion of LTA4 to LTB4 and plays a key role in innate immune responses. Inhibition of this enzyme can be a valid method in the treatment of inflammatory response exhibited through LTB4.</P>
Single-drop microextraction in bioanalysis.
Choi, Kihwan,Kim, Jihye,Chung, Doo Soo Future Science 2011 Bioanalysis Vol.3 No.7
<P>Bioanalysis usually requires a preparation procedure for sample cleanup or preconcentration. Conventional sample preparation techniques are often time consuming and labor intensive. Among recent progress in sample preparation, single drop microextraction (SDME) is one of the most efficient techniques providing both sample cleanup and preconcentration capabilities. In SDME, analytes are extracted from a sample solution into an acceptor drop and the drop is introduced to subsequent analysis. Since the volume of the acceptor drop is 1-10 µl or less, the consumption of solvents can be minimized and the preconcentration effect is enhanced. In this review, the basic principles of two-phase and three-phase SDME are described briefly and then recently developed modes of SDME, coupling with analytical instruments, and methods to enhance the drop stability are discussed. Recent applications of SDME to biological samples, including urine, blood and saliva, for the analysis of drugs, metal ions and biomarkers are reviewed.</P>
Oh, Myung Jin,Hua, Serenus,Kim, Bum Jin,Jeong, Ha Neul,Jeong, Seung Hyup,Grimm, Rudolf,Yoo, Jong Shin,An, Hyun Joo Future Science 2013 Bioanalysis Vol.5 No.5
<P>Erythropoietin is a therapeutic glycoprotein that stimulates red blood cell production. The quality, safety and potency of recombinant erythropoietins are determined largely by their glycosylation. Small variations in cell culture conditions can significantly affect the glycosylation, and therefore the efficacy, of recombinant erythropoietins. Thus, detailed glycomic analyses are necessary to assess biotherapeutic quality. We have developed a platform for qualitative and quantitative glycomic analysis of recombinant erythropoietins.</P>
Investigation of metabolite alteration in dimethylnitrosamine-induced liver fibrosis by GC-MS.
Ju, Hyun Kyoung,Chung, Ha Wook,Lee, Hee-Seung,Lim, Johan,Park, Jeong Hill,Lim, Sung Cil,Kim, Joon Mee,Hong, Soon-Sun,Kwon, Sung Won Future Science 2013 Bioanalysis Vol.5 No.1
<P>A metabolomic study of biomarkers associated with dimethylnitrosamine (DMN)-induced hepatic fibrosis in Sprague-Dawley rats was performed using GC-MS. The clinical chemistry of the collected blood and the histopathology of excised liver samples were examined, and urine samples were prepared by solvent extraction.</P>
Design, synthesis and cellular metabolism study of 4'-selenonucleosides.
Yu, Jinha,Sahu, Pramod K,Kim, Gyudong,Qu, Shuhao,Choi, Yoojin,Song, Jayoung,Lee, Sang Kook,Noh, Minsoo,Park, Sunghyouk,Jeong, Lak Shin Future Science 2015 Future medicinal chemistry Vol.7 No.13
<P>4'-seleno-homonucleosides were synthesized as next-generation nucleosides, and their cellular phosphorylation was studied to confirm the hypothesis that bulky selenium atom can sterically hinder the approach of cellular nucleoside kinase to the 5'-OH for phosphorylation.</P>