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- 저자 : geret, Bertrand (검색결과 1 건)
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Yamaoka, Yasuyo,Shin, Seungjun,Choi, Bae Young,Kim, Hanul,Jang, Sunghoon,Kajikawa, Masataka,Yamano, Takashi,Kong, Fantao,Lé,geret, Bertrand,Fukuzawa, Hideya,Li-Beisson, Yonghua,Lee, Youngsook American Society of Plant Biologists 2019 The Plant cell Vol.31 No.5
<P>The mRNA of a Chlamydomonas bZIP transcription factor is spliced by CrIRE1 under ER stress, and the resulting protein protects Chlamydomonas cells from ER stress by modulating lipid remodeling.</P><P>Endoplasmic reticulum (ER) stress is caused by the stress-induced accumulation of unfolded proteins in the ER. Here, we identified proteins and lipids that function downstream of the ER stress sensor INOSITOL-REQUIRING ENZYME1 (CrIRE1) that contributes to ER stress tolerance in Chlamydomonas (<I>Chlamydomonas reinhardtii</I>). Treatment with the ER stress inducer tunicamycin resulted in the splicing of a 32-nucleotide fragment of a basic leucine zipper 1 (bZIP1) transcription factor (<I>CrbZIP1</I>) mRNA by CrIRE1 that, in turn, resulted in the loss of the transmembrane domain in CrbZIP1, and the translocation of CrbZIP1 from the ER to the nucleus. Mutants deficient in <I>CrbZIP1</I> failed to induce the expression of the unfolded protein response genes and grew poorly under ER stress. Levels of diacylglyceryltrimethylhomoserine (DGTS) and pinolenic acid (18:3Δ5,9,12) increased in the parental strains but decreased in the <I>crbzip1</I> mutants under ER stress. A yeast one-hybrid assay revealed that CrbZIP1 activated the expression of enzymes catalyzing the biosynthesis of DGTS and pinolenic acid. Moreover, two lines harboring independent mutant alleles of <I>Chlamydomonas desaturase</I> (<I>CrDES</I>) failed to synthesize pinolenic acid and were more sensitive to ER stress than were their parental lines. Together, these results indicate that <I>CrbZIP1</I> is a critical component of the ER stress response mediated by CrIRE1 in Chlamydomonas that acts via lipid remodeling.</P>
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