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( Zhilin Wang ),( Li Wang ),( Zhuang Chen ),( Xianyong Ma ),( Xuefen Yang ),( Jian Zhang ),( Zongyong Jiang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6
Probiotics are considered as the best effective alternatives to antibiotics. The aim of this study was to characterize the probiotic potential of lactobacilli for use in swine farming by using in vitro evaluation methods. A total of 106 lactic acid bacterial isolates, originating from porcine feces, were first screened for the capacity to survive stresses considered important for putative probiotic strains. Sixteen isolates showed notable acid and bile resistance, antibacterial activity, and adherence to intestinal porcine epithelial cells (IPEC-1). One isolate, LR1, identified as Lactobacillus reuteri, was selected for extensive study of its probiotic and functional properties in IPEC-1 cell models. L. reuteri LR1 exhibited good adhesion to IPEC-1 cells and could inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells. L. reuteri LR1 could also modulate transcript and protein expression of cytokines involved in inflammation in IPEC-1 cells; the Lactobacillus strain inhibited the ETEC-induced expression of proinflammatory transcripts (IL-6 and TNF-α) and protein (IL-6), and increased the level of anti-inflammatory cytokine (IL-10). Measurement of the permeation of FD-4 showed that L. reuteri LR1 could maintain barrier integrity in monolayer IPEC-1 cells exposed to ETEC. Immunolocalization experiments showed L. reuteri LR1 could also prevent ETEC-induced tight junction ZO-1 disruption. Together, these results indicate that L. reuteri LR1 exhibits desirable probiotic properties and could be a potential probiotic for use in swine production.
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Quan Li,Zongyong Wang,Ruting Lin,Shuai Li,Chao Wang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.11
This paper adopts the standard k-ε model and mixture model of the FLUENT code to numerically analyze the components characteristics of the gravity separator. It intends to involve several combinations of four types of rectifiers and two types of coalescers of corrugated plates to optimize the structure of the components. Numerical simulation analysis is carried out on the synergistic effect of the reflective inlet component, the rectifier of square grids, and the coalescer of perforated corrugated plates under variable working conditions. The results illustrate that when the distance between the rectifier and the separator’s head is L 1 = 3/4 D, the width L 2 = 1/4 D, and the plate spacing L 3 = 1/10 D, the separation effect gets improved. Additionally, the perforated corrugated plates prove better effects of rectification and coalescence. And when the distance between the coalescer and the head is L 4 = 25/183 L and the elevation angle α = 2.5°, the separation performance will be further improved. What’s more, the influence of the Reynolds number (Re i , from 5640 through 8297 in the inlet component) and the volume fraction of the dispersed phase (from 5 % through 30 %) on the separation effects of various combination conditions of the internal components has also been investigated. As the former increases, the combined of reflective inlet component, rectifier and coalescer will perform the best separation effects. As the latter increases, the separation efficiency of low-oil ratio of oil-water mixture of the jointly structured separator will increase the most.
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Huibo Meng,Xiuhui Jiang,Yanfang Yu,Zongyong Wang,Jianhua Wu 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.5
The laminar flow and chaotic mixing characteristics of a high-viscosity fluid in static mixers with staggered perforated helical segments were numerically investigated in the range of Re=0.1-150. The numerical results of pressure drop of Kenics static mixer have a good agreement with the reported data from the literature. The effects of aspect ratio Ar and Reynolds number on the mixing performance of Modified Kenics Static Mixers (MKSM) were evaluated by Darcy friction coefficient, shear rate, stretching rate, and Lyapunov exponent, respectively. The product of f×Re for MKSM linearly increased with the increase of Re, but it was constant under Re<10. The values of shear rate in the first perforated hole of mixing elements gradually became much larger by 1.10%-11.78% than those in the second perforated hole with the increasing Re. With the increase of dimensionless axial mixing length, the stretching rate increased linearly and the sensitivity for initial condition gradually weakened. A larger Ar is beneficial for micro-mixing in creeping flow. The average Lyapunov exponent linearly increases with the increase of Re. The profiles of Lyapunov exponent at different dimensionless perforated diameter (d/W) and perforated spacing (s/W) indicate that the chaotic mixing in MKSM is much more sensitive to d/W than s/W. A dimensionless parameter η taking into account the mixing degree and pressure drop was employed to evaluate the mixing efficiency. The optimization of perforated helical segments with the highest mixing efficiency at Re=100 was d/W=0.55 and s/W=1.2.
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