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Yang Yang,Yuwei Zhang,Zijin Wang,Xuefeng Si,Jinran Wu 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.2
Fault-tolerant control (FTC) of a non-affine system with uncertainties is one of critical issues in nonlinear control. In this paper, in the presence of unknown actuator failures, an event-trigger-based FTC scheme is proposed for such a nonlinear system with predefined performance. For actuator failures, a compensation mechanism is designed to alleviate their impacts. By utilizing a predefined performance function, higher tracking accuracy can be obtained. Meanwhile, an event-triggered mechanism with a time-varying threshold, depending on tracking error, reduces the number of communications for a controller-to-actuator channel. An adaptive event-triggered function is then proposed with the compensation mechanism to improve the self-adjusting ability of the triggered function. Also, extended state observers and tracking differentiators are utilized to reconstruct unknown dynamics of the system and to simplify high-order derivation of virtual control laws, respectively. The stability of the closed-loop system is analyzed by input-to-state practically stability. Finally, two simulation results are supplied to verify the effectiveness of the proposed control method.
Cao Hongli,Zhang Guosheng,Ma Hui,Xue Zhongwen,Huo Ran,Wang Kun,Liu Zijin 한국미생물·생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.1
Refractory infections, such as hospital-acquired pneumonia, can be better diagnosed with the assistance of precise methicillin-resistant Staphylococcus aureus (MRSA) testing. However, traditional methods necessitate high-tech tools, rigorous temperature cycling, and the extraction of genetic material from MRSA cells. Herein, we propose a sensitive, specific, and extraction-free strategy for MRSA detection by integrating allosteric probe-based target recognition and exonuclease-III (ExoIII)-enhanced color reaction. The penicillin-binding protein 2a (PBP2a) aptamer in the allosteric probe binds with MRSA to convert protein signals to nucleic acid signals. This is followed by the DNA polymerase-assisted target recycle and the production of numerous single-strand DNA (ssDNA) chains which bind with silver ion (Ag+ ) aptamer to form a blunt terminus that can be identified by Exo-III. As a result, the Ag+ aptamer pre-coupled to magnetic nanoparticles is digested. After magnetic separation, the Ag+ in liquid supernatant catalyzes 3,3’,5,5’-tetramethylbenzidine (TMB) for a color reaction. In addition, a concentration of 54 cfu/mL is predicted to be the lowest detectable value. Based on this, our assay has a wide linear detection range, covering 5 orders of magnitude and demonstrating a high specificity, which allows it to accurately distinguish the target MRSA from other microorganisms.