Stem cells from human exfoliated deciduous teeth attenuate trigeminal neuralgia in rats by inhibiting endoplasmic reticulum stress

Yang, Zhijie,Wang, Chun,Zhang, Xia,Li, Jing,Zhang, Ziqi,Tan, Zhao,Wang, Junyi,Zhang, Junyang,Bai, Xiaofeng The Korean Pain Society 2022 The Korean Journal of Pain Vol.35 No.4

Background: The treatment of trigeminal neuralgia remains a challenging issue. Stem cells from human exfoliated deciduous teeth (SHED) provide optimized therapy for chronic pain. This study aimed to investigate the mechanisms underlying the attenuation of trigeminal neuralgia by SHED. Methods: Trigeminal neuralgia was induced by chronic constriction injury of the infraorbital nerve. The mechanical threshold was assessed after model establishment and local SHED transplantation. Endoplasmic reticulum (ER) morphology and Caspase12 expression in trigeminal ganglion (TG) was evaluated as well. BiP expression was observed in PC12 cells induced by tunicamycin. Results: The local transplantation of SHED could relieve trigeminal neuralgia in rats. Further, transmission electron microscopy revealed swelling of the ER in rats with trigeminal neuralgia. Moreover, SHED inhibited the tunicamycin-induced up-regulated expression of BiP mRNA and protein in vitro. Additionally, SHED decreased the up-regulated expression of Caspase12 mRNA and protein in the TG of rats caused by trigeminal neuralgia after chronic constriction injury of the infraorbital nerve mode. Conclusions: This findings demonstrated that SHED could alleviate pain by relieving ER stress which provide potential basic evidence for clinical pain treatment.

Analysis on the Digging of Social Network Based on User Search Behavior

Junyi Du,Zhiyong Zhang,Changwei Zhao 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.5

The Key to The Future Development of Interactive Art - Virtual Reality Technology

Wang, Junyi,Wang, Yuanyuan,Zhang, Ning,Lee, Eung-Joo,Yabin, Li,Liao, Gang Korea Multimedia Society 2018 The journal of multimedia information system Vol.5 No.4

Interactive art is an art form that closely links art creators and art participants. It has been doomed with advanced technology from the very beginning. Virtual Reality is a technology which born in the background of the maturity of computer technology. Its birth has brought immeasurable possibilities for interactive art, especially the interactivity and multi-sensory experience. This paper discusses the current development status of interactive art in the field of interactive art through the characteristics of virtual reality technology and the future development of this technology will bring to interactive art.

<i>In Situ</i> Activation of Nitrogen-Doped Graphene Anchored on Graphite Foam for a High-Capacity Anode

Ji, Junyi,Liu, Jilei,Lai, Linfei,Zhao, Xin,Zhen, Yongda,Lin, Jianyi,Zhu, Yanwu,Ji, Hengxing,Zhang, Li Li,Ruoff, Rodney S. American Chemical Society 2015 ACS NANO Vol.9 No.8

<P>We report the fabrication of a three-dimensional free-standing nitrogen-doped porous graphene/graphite foam by <I>in situ</I> activation of nitrogen-doped graphene on highly conductive graphite foam (GF). After <I>in situ</I> activation, intimate “sheet contact” was observed between the graphene sheets and the GF. The sheet contact produced by <I>in situ</I> activation is found to be superior to the “point contact” obtained by the traditional drop-casting method and facilitates electron transfer. Due to the intimate contact as well as the use of an ultralight GF current collector, the composite electrode delivers a gravimetric capacity of 642 mAh g<SUP>–1</SUP> and a volumetric capacity of 602 mAh cm<SUP>–3</SUP> with respect to the whole electrode mass and volume (including the active materials and the GF current collector). When normalized based on the mass of the active material, the composite electrode delivers a high specific capacity of up to 1687 mAh g<SUP>–1</SUP>, which is superior to that of most graphene-based electrodes. Also, after ∼90 s charging, the anode delivers a capacity of about 100 mAh g<SUP>–1</SUP> (with respect to the total mass of the electrode), indicating its potential use in high-rate lithium-ion batteries.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2015/ancac3.2015.9.issue-8/acsnano.5b03888/production/images/medium/nn-2015-03888k_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn5b03888'>ACS Electronic Supporting Info</A></P>

Generation of B-Doped Graphene Nanoplatelets Using a Solution Process and Their Supercapacitor Applications

Han, Jongwoo,Zhang, Li Li,Lee, Seungjun,Oh, Junghoon,Lee, Kyoung-Seok,Potts, Jeffrey R.,Ji, Junyi,Zhao, Xin,Ruoff, Rodney S.,Park, Sungjin American Chemical Society 2013 ACS NANO Vol.7 No.1

<P>Chemically modified graphene (CMG) nanoplatelets have shown great promise in various applications due to their electrical properties and high surface area. Chemical doping is one of the most effective methods to tune the electronic properties of graphene materials. In this work, novel B-doped nanoplatelets (borane-reduced graphene oxide, B-rG-O) were produced on a large scale <I>via</I> the reduction of graphene oxide by a borane-tetrahydrofuran adduct under reflux, and their use for supercapacitor electrodes was studied. This is the first report on the production of B-doped graphene nanoplatelets from a solution process and on the use of B-doped graphene materials in supercapacitors. The B-rG-O had a high specific surface area of 466 m<SUP>2</SUP>/g and showed excellent supercapacitor performance including a high specific capacitance of 200 F/g in aqueous electrolyte as well as superior surface area-normalized capacitance to typical carbon-based supercapacitor materials and good stability after 4500 cycles. Two- and three-electrode cell measurements showed that energy storage in the B-rG-O supercapacitors was contributed by ion adsorption on the surface of the nanoplatelets in addition to electrochemical redox reactions.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2013/ancac3.2013.7.issue-1/nn3034309/production/images/medium/nn-2012-034309_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn3034309'>ACS Electronic Supporting Info</A></P>

Morphological study on the mouthparts of four adult Aphodiinae beetles (Coleoptera: Aphodiinae)

Lu Ying,Zhang Junyi,Fang Hong 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

Aphodiinae belong to Scarabaeidae, which are important environment-cleaning insects. In this study, the ul trastructural characteristics of the mouthparts (including the epipharynx, mandible and maxillae) of adult Aphodiinae, namely, Phaeaphodius rectus, Colobopterus erraticus, Paracrossidius transmontanus and Rhyparus sp., were systematically studied. The results are as follows: I. Rhyparus sp. exhibits saprophagy, while the others exhibit coprophagy. II. The three kinds of coprophagous Aphodiinae are all likely to feed on fresh dung. Among them, C. erraticus not only feeds on dung with the highest moisture content but also ingests solid food particles with the smallest diameter. Saprophagous Rhyparus sp. can ingest solid food particles with the largest diameter but feeds on humus with the lowest moisture content. The mouthpart structures of the Aphodiinae were highly consistent with their feeding habits. III. The findings support the utility of the epipharynx as an important morphological characteristic for classification of Aphodiinae at the genus and higher taxonomic levels. However, whether this inference is valid still needs to be verified by molecular biological data. In conclusion, the results of this study not only complement morphological information on the mouthparts of Aphodiinae but also provide basic data for further research on the evolution of the mouthparts of Aphodiinae.

Degrees of Freedom of Multi-Cell MIMO Interference Broadcast Channels With Distributed Base Stations

( Hongbing Huang ),( Junyi Liu ),( Yi Zhang ),( Qing Cai ),( Bowei Zhang ),( Fengwen Jiang ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.2

In this paper, we investigate the degrees of freedom (DoF) of a multi-cell multi-user multiple-input multiple-output (MIMO) interference broadcast channel (IBC) with non-cooperation distributed base stations (BS), where each BS serves users of its corresponding cell. When all BSs simultaneously transmit their own signals over the same frequency band in the MIMO IBC, the edge users in each cell will suffer the inter-cell interference (ICI) and inter-user interference (IUI) signals. In order to eliminate the ICI and IUI signals, a distributed space time interference alignment (DSTIA) approach is proposed where each BS has only limited access to distributed moderately-delay channel state information at the transmitter (CSIT). It is shown that the DSTIA scheme can obtain the appreciate DoF gains. In addition, the DoF upper bound is asymptotically achievable as the number of antenna at each BS increases. It is shown that the DSTIA method can get DoF gains over other interference alignment schemes with delayed CSIT in literature. Moreover, the DSTIA method can attain higher DoFs than the IA schemes with global CSIT for certain antenna configurations.

Vibration characteristics investigation of mistuned blisks with receptance substructure component modal synthesis method

Bin Bai,Junyi Zhang,Yanchao Cui,Han Li 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.7

To improve the simulation efficiency of mistuned blisk, a method called receptance substructure component modal synthesis method (RSCMSM) is proposed to reduce its degrees of freedom (DOFs). The advantage of this method is that only the interface DOFs need to be solved, which observably enhances the computational efficiency. The modal frequencies, maximum modal shape and frequency response function are calculated via RSCMSM. It is seen that the smooth frequency band is governed by blade vibration and the steep frequency band is governed by disk or bladed-disk coupling vibration. In addition, a peak is observed for the tuned blisk but many peaks appear for the mistuned blisk and many small wave crests are observed near the peak. To verify validity of this method, the computational time of RSCMSM is compared with high fidelity finite element method (HFFEM) and classical substructure component modal synthesis method (CSCMSM), which manifests that the computational efficiency increases by 32.19 %-80.82 % than that of HFFEM when the mistuned level is 0 %~5 %. Moreover, computational efficiency of RSCMSM is increased by 0.85 %-7.56 % than that of CSCMSM. The validity of RSCMSM is verified for calculating the complex mechanical structure.

속도선택에 기초한 주행위험도 평가모델 개발

김원철(Kim, Won-Cheol),Junyi Zhang,Akimasa Fujiwara 대한교통학회 2010 대한교통학회 학술대회지 Vol.62 No.-

Gemcitabine Inhibits the Progression of Pancreatic Cancer by Restraining the WTAP/MYC Chain in an m6A-Dependent Manner

Pei Cao,Weigang Zhang,Junyi Qiu,Zuxiong Tang,Xiaofeng Xue,Tingting Feng 대한암학회 2024 Cancer Research and Treatment Vol.56 No.1

Purpose Pancreatic cancer (PC) is a common malignant tumor of the digestive system, and its 5-year survival rate is only 4%. N6-methyladenosine (m6A) RNA methylation is the most common post-transcriptional modification and dynamically regulates cancer development, while its role in PC treatment remains unclear.Materials and Methods We treated PC cells with gemcitabine and quantified the overall m6A level with m6A methylation quantification. Real-time quantitative reverse transcription polymerase chain reaction and Western blot analyses were used to detect expression changes of m6A regulators. We verified the m6A modification on the target genes through m6A-immunoprecipitation (IP), and further <i>in vivo</i> experiments and immunofluorescence (IF) assays were applied to verify regulation of gemcitabine on Wilms’ tumor 1–associated protein (WTAP) and MYC.Results Gemcitabine inhibited the proliferation and migration of PC cells and reduced the overall level of m6A modification. Additionally, the expression of the “writer” WTAP was significantly downregulated after gemcitabine treatment. We knocked down WTAP in cells and found target gene MYC expression was significantly downregulated, m6A-IP also confirmed the m6A modification on MYC. Our experiments showed that m6A-MYC may be recognized by the “reader” IGF2BP1. <i>In vivo</i> experiments revealed gemcitabine inhibited the tumorigenic ability of PC cells. IF analysis also showed that gemcitabine inhibited the expression of WTAP and MYC, which displayed a significant trend of co-expression.Conclusion Our study confirmed that gemcitabine interferes with WTAP protein expression in PC, reduces m6A modification on MYC and RNA stability, thereby inhibiting the downstream pathway of MYC, and inhibits the progression of PC.