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      저자 : Zahra Kharat (검색결과 1 건)
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        Herbal Extract Loaded Chitosan/PEO Nanocomposites as Antibacterial Coatings of Orthopaedic Implants

        Zahra Kharat,Minoo Sadri,Mahboubeh Kabiri 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4

        One of the most substantial complications in orthopedic surgeries is implant associated infection. While theimplant interface can be a permissive surface for bacterial colonization, an appropriate coating on the implant can effectivelyhinder biofilm formation. To this aim, henna and thyme extract loaded into a chitosan (CS)/polyethylene oxide (PEO) basednanofibrous hydrogel. CS/PEO/henna and CS/PEO/thymus extract nanofibers were fabricated via two-nozzle electrospinning. The proper weight ratio of CS/PEO/herbal extract was optimized. The appropriate ratio of CS/PEO and the most suitableextract concentrations was obtained to be 7/3 (v/v) and 1 % (v/v), respectively. Contact angle measurements showed anappropriate degree of hydrophilicity in all groups including herbal loaded electrospun nanosheets. Tensile strengthmeasurements showed that inclusion of henna and thymus made minor changes in tensile strength and strain of herbal loadednanofiber, compared to control CS/PEO fibers. Then antibacterial tests were performed and the fibers either by themselves orwhen used as coating on orthopedic screws exhibited a good inhibition zone against both gram negative and gram positivebacteria. The coatings showed no adverse effect on the red blood cells in leading to hemolysis. Cytocompatibility assayindicated that the prepared nanofibrous hydrogel coating was absolutely biocompatible as tested with fibroblast cells. Theresults of this study indicated that our henna and thyme extracts loaded CS/PEO coatings can be used in various medicalfields such as wound dressing and implantation coating to prevent bacterial adhesion, growth and generally, infection.

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