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Apoptosis Progression in the Hair Cells in the Organ of Corti of GJB2 Conditional Knockout Mice
Yanping Zhang,Xiaoqiang Zhang,Lina Li,Yurui Sun,Jianhe Sun 대한이비인후과학회 2012 Clinical and Experimental Otorhinolaryngology Vol.5 No.3
Objectives. Apoptosis may play an important role in the mechanism underlying the GJB2 gene conditional knockout (cCx26) mice cochlear cell death. The objective of this study was to explore the the damage mode of the outer hair cells (OHCs) and its real time point of apoptosis and provide information to further explore the role of apoptosis in the happening of hearing loss in cCx26 mice. Methods. Cochleae from mice at various developmental stages (P8, P12, and P21) were dissected out and first used to be observed under the scanning electron microscope (SEM). Basilar membranes from mice at P8, P14, P18, and P21were stained by fluorescein isothiocyanate-conjugated phalloidin and propidium iodide (PI) and examined under confocal microscope. Results. The loss of OHCs of cCx26 knockout mice was first set between P12 and P21 under SEM. Whole mount phalloidin and PI staining revealed that obvious apoptotic appearance of the OHCs surface morphology was observed at P18. Conclusion. Typical apoptotic morphology was found in the OHCs in the organ of Corti of the cCx26 mice at P18. This may provide information to further study the role of apoptosis in the occurrence of hearing loss of cCx26 mice. Objectives. Apoptosis may play an important role in the mechanism underlying the GJB2 gene conditional knockout (cCx26) mice cochlear cell death. The objective of this study was to explore the the damage mode of the outer hair cells (OHCs) and its real time point of apoptosis and provide information to further explore the role of apoptosis in the happening of hearing loss in cCx26 mice. Methods. Cochleae from mice at various developmental stages (P8, P12, and P21) were dissected out and first used to be observed under the scanning electron microscope (SEM). Basilar membranes from mice at P8, P14, P18, and P21were stained by fluorescein isothiocyanate-conjugated phalloidin and propidium iodide (PI) and examined under confocal microscope. Results. The loss of OHCs of cCx26 knockout mice was first set between P12 and P21 under SEM. Whole mount phalloidin and PI staining revealed that obvious apoptotic appearance of the OHCs surface morphology was observed at P18. Conclusion. Typical apoptotic morphology was found in the OHCs in the organ of Corti of the cCx26 mice at P18. This may provide information to further study the role of apoptosis in the occurrence of hearing loss of cCx26 mice.
Underwater contactless wet‑mateable connector using bowl‑shaped coils
Qichao Zhao,Tianlei Wang,Hui Zhi,Xin Wu,Yurui Zhang,Canjun Yang,Yanhu Chen 전력전자학회 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.7
Wet-mateable connectors enabling power and communication for underwater equipment are greatly favored for underwater systems. However, conventional underwater wet-mateable connectors with electrical contact conductors have the features of high cost, limited mate/de-mate times, and high risk of ground faults when being applied underwater, which limits their used. In this paper, a contactless underwater wet-mateable connector with bowl-shaped couplers was proposed, and an equivalent circuit for inductive power transmission was modeled and analyzed. Then the mathematical expression of the magnetic induction intensity of the bowl-shaped coil was derived. The parameters including co , zco , and s0 of the coil were analyzed and optimized to attain better performance and lower cost. A prototype was built and tested. Results showed that it can transmit power up to 1125 W with an efficiency of 85%. In addition, it can achieve a data rate up to 6 MB/s under a water pressure of 20 MPa.