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Yuanxia Sun,Yupin Guan,Shigeru Hayakawa,Masahiro Ogawa,Supaporn Naknukool,Yoshiyuki Matsumoto 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.2
Solid wastes of freshwater clam in food processing, including mainly mantle, were used as a raw material for the recovery of bioactive peptides related to angiotensin I-converting enzyme (ACE) inhibitor. Among the primary hydrolysates of dried mantle (DM), the peptides corresponding to hydrolysates using 2 crude peptidases exhibited a strong ACE inhibitory activity (IC_50, 0.23 mg/mL), and recovery efficiency of soluble materials and their protein content were considerably large with 42.65% and 468.6 mg/g, respectively. The ACE inhibitory activity of all secondary hydrolysates digested by pepsin and trypsin was significantly increased as compared to primary hydrolysates. Furthermore, the peptic secondary hydrolysates were fractionated by gel filtration and reverse phase-HPLC (RP-HPLC) and characterized by matrix-assisted laser desorption-ionization time of flight/mass spectrometry (MALDI-TOF/MS). These peptides with molecular weight of less than 1 kDa possessed the stronger ACE inhibitory effect, and their inhibitory pattern was found to be competitive. The results showed that the DM hydoplysates might be utilized as a rich source of bioactive peptide.
Yan Zeng,Huan Zhang,Yuping Guan,Lili Zhang,Yuanxia Sun 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.2
D-Psicose, recognized as a noncaloric sweetener,has shown a great potential in food industry. In the present study, D-psicose and D-fructose were used to modify bovine β-lactoglobulin (β-Lg) through Maillard reaction. The Maillard reaction process and the physicochemical and structural properties of the modified proteins were also investigated. The result showed that compared to Dfructose,D-psicose played a more effective role in the Maillard reaction, especially after the initial stage of the reaction. Moreover, the modified β-Lg with D-psicose had more polymeric compounds, higher antioxidant activity,but lower thermal stability than that with D-fructose. These findings, especially the structural changes of the modified proteins, supplied detail information on the Maillard reaction of D-psicose, and could provide some guidance to the practical applications of this rare sugar on food industry.
Yan Zeng,Ying Zhang,Lijiao Zhang,Shimao Cui,Yuanxia Sun 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.5
P20, P40, P60, and P80 polysaccharide fractions were isolated from the spent rice substrate of Cordyceps militaris and structure and antioxidant and immunomodulation activities were studied. P20 contained mainly starch from rice. Purified P40 was an α-1, 4-glucan with some α-1, 4, 6 l ink ed branches. P60 containing t-Galp and 1, 2-Manp had the highest FRAP value of 78.4 μmol Fe2+/g. P80 containing t-Galp, 1,6-Galp, and 1, 2-Manp had the highest TEAC and ORACFL values and the lowest IC50 value of 0.32 mg/mL against DPPH radicals. All polysaccharides showed good biosafety in an MTT assay. P40, P60, and P80 stimulated NO production in a dose-dependent manner. Polysaccharides from the spent substrate of C. militaris can be used as safe antioxidants and immunomodulators and can be used for new applications in the food industry.