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( Yoonhang Lee ),( Nameun Kim ),( Ju-yeop Lee ),( Hyoyeong Kang ),( Minji Sung ),( Young-bin Yu ),( Kyunghoi Kim ),( Jae-young Je ),( Hyun-woo Kim ),( Ju-chan Kang ),( Do-hyung Kim ) 한국어병학회 2021 한국어병학회지 Vol.34 No.2
This is the first report describing acute mass mortality occurred in juvenile olive flounder (Paralichthys olivaceus) caused by gas bubble disease (GBD). A total of 610 fish (average weight = 35 g), which were more than half of the fish acclimated at 17℃ in an aquarium, were killed within two days of acclimation. The dead and moribund fish showed excessively opened opercula and mouths, and occasionally, severe exophthalmia. Through microscopic observation, numerous gas emboli were found in the gills of the dead and live fish, while the fish were not infected with any microbial pathogens. The dissolved oxygen (DO) saturation level of the rearing water and seawater nearby the facility reached 145% and 286%, respectively, whereas other water quality parameters (such as salinity, pH, and chemical oxygen demand) were normal. The extreme saturation rate of seawater in the shore nearby seemed to be due to an enormous algal bloom that occurred there. Through molecular identification based on 18S rDNA sequences, the most dominant algal species was most closely related to Ulva californica (99.87% sequence identity) followed by U. prolifera, U. linza, and U. curvata (99.81%). Therefore, it can be concluded that supersaturated seawater due to mass algal bloom caused gas bubble disease in the olive flounder, leading to mass mortality. After technical adjustment, such as increased aeration, lowered water circulation rate, and inlet water filtration using micro-pore carbon filters, the DO level became normal, no further mortality occurred and the status of the fish was stabilized.
Differential proteome profile of gill and spleen in three pathogen-infected Paralichthys olivaceus
A-Reum Lee,Hyunsu Kim,Kyung-Yoon Jeon,고은지,Ahran Kim,Nameun Kim,HyeongJin Roh,Yoonhang Lee,Jiyeon Park,Do‑Hyung Kim,Yung Hyun Choi,김석만,김희수,Mee Sun Ock,차희재 한국유전학회 2021 Genes & Genomics Vol.43 No.7
Background Olive founder (Paralichthys olivaceus) is one of the major cultured fsh species in Asia including Korea. However, the mass mortality of olive founder caused by various pathogens leads to huge economic loss. The pathogens that lead to fsh mortality include parasites, bacteria, and viruses that can cause various kinds of diseases. Objective The purpose of this study was to investigate the protein expression patterns in the gills and spleens of olive founder after artifcial infection. We hypothesized that proteomics levels in gills and spleen may be diferentially expressed depending on infectious agents. Methods To investigate the expression pattern of proteins in gills and spleens, olive founders were experimentally infected with VHSV (virus), S. parauberis (bacteria), or M. avidus (pathogenic ciliate). Proteins were extracted from the gills and spleens of infected olive founder. We used 2-DE analysis with LC–MS/MS to investigate proteome changes in infected olive founders. Results The results of the LC–MS/MS analyses showed diferent protein expression profles depending on pathogenic sources and target organs. Proteins related to cytoskeletal structure like keratin, calmodulin and actin were mostly expressed in the infected gills. Proteins involved in the metabolism pathway like glycolysis were expressed mainly in the spleens. The protein profles of S. parauberis and VHSV infection groups had many similarities, but the profle of the M. avidus infection group was greatly diferent in the gill and spleen. Conclusion Our results indicate that measures according to the characteristics of each pathogen are necessary for disease prevention and treatment of farmed fsh.
포르말린과 청색 LED 병용처리에 의한 항스쿠티카 효과
강문경,이주엽,이윤항,김도형,Kang, Mun-Gyeong,Lee, Ju-Yeop,Lee, Yoonhang,Kim, Do-Hyung 한국어병학회 2022 한국어병학회지 Vol.35 No.1
Scuticociliatosis caused by Miamiensis avidus is a very important parasitic disease in olive flounder farming industry. The aim of this study was to determine effect of combined treatment with blue LED (light-emitting diode) illumination and formalin on olive flounder (Paralichthys olivaceus) infected with M. avidus. Different intensity of 405 nm LED (20, 40, and 60 μmol·m<sup>-2</sup>·s<sup>-1</sup>) was illuminated on 2.2×10<sup>4</sup> cells/well of M. avidus in a 24 well microplate for 24 h. Also, 2.4×10<sup>4</sup> cells/well of M. avidus were exposed to varying combinations of 60 μmol·m<sup>-2</sup>·s<sup>-1</sup> of 405 nm LED and serial 10-fold dilutions of formalin (from 10 to 100 ppm) for 15, 30, 45, and 60 min. Surviving M. avidus were counted using a hemocytometer. For in vivo test, flounder acclimatized at 11-12 practical salinity unit (psu) were challenged with 2×10<sup>6</sup> cells/ml of M. avidus by immersion method for 1 h. Then, fish were moved and divided into four groups; "F" group, treated with formalin at 50 ppm; "L" group, treated with 60 μmol·m<sup>-2</sup>·s<sup>-1</sup> of 405 nm LED; "C" group, treated with combination of the two methods; and the control group. After treatment for 30 min, fish were transferred to new tanks (salinity = 11-12 psu) and observed for 3 weeks. As a result, illumination of 405 nm LED at 60 μmol·m<sup>-2</sup>·s<sup>-1</sup> killed 100% of M. avidus after 12 h, while 67% and 90% of the scuticociliate died at 20 and 40 μmol·m<sup>-2</sup>·s<sup>-1</sup>, respectively, after 24 h exposure. One hundred percent of M. avidus was killed at 90, 80, 80 and 70 ppm after exposure to formalin for 15, 30, 45 and 60 min, respectively. However, combined method (e.g., 60 μmol·m<sup>-2</sup>·s<sup>-1</sup> of 405 nm-LED plus 50 ppm formalin) killed the parasite within 30 min. From in vivo test, similarly, survival rates of fish challenged with M. avidus were 100%, 43%, 29% and 0% in the C, F, L, and control groups, respectively. Results obtained in this study demonstrates that the combined treatment method has clear synergistic effect on scuticociliatosis in fish.