레이저 박피술의 피부면적 감소효과와 레이저 punch out 병변의 치유에 대한 실험적 연구

강동희,윤을식,구상환,안덕선,박승하 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

An experimental study was designed to analyze the surface reduction effect of ultrapulse carbon dioxide laser on the skin of guinea pigs receiving laser resurfacing. Two squares of skin measuring 3x3 ㎠ were designed on each side of the abdomen of guinea pigs(n=10). Five animals received laser resurfacing on their abdomen at energy of 250mJ/5 watt, and five other animals received 500 mJ/5 watt. On each animal, the left side square was treated with one pass of a CO2 laser, and the right side q=square was treated with two passes using a C.P.G. scanner. The size of the resurfacing area was determined by celluloid overlays at 1,2,3,4 and 6 weeks. Immediately following laser resurfacing, the area was decreased to 69%(left side) and 56%(right side) of the designed area in the 250 mJ/5 watt group, and 59% and 40% in the 500mJ/5 watt group. After 6 weeks, the resurfaced area had been reduced to 70% and 56% of the designed area in the 250mJ/5 watt group, and 60% and 40% in the 500mJ/5 watt group. As a result, the effect of surface reduction by CO2 laser resurfacing increased at a higher power and when more passes of the ultrapulse carbon dioxide laser were applied. The laser treated skin continued to maintain its contracted dimensions. These findings suggest that ultrapulse carbon dioxide laser may be an effective treatment method for rhytides. Another experiment was performed on multiple punch-out lesions on guinea pig skin(n=2) with 1,2,3 mm collimated hand pieces for the purpose of determining a new laser treatment method. After 4 weeks, on gross and histological examination, there were no differences in the healing of multiple punch-out lesions and the healing of punch-out lesions was complete without any scarring. These findings suggest that the laser punch-out method can be used for the treatment of acne scars.

실리카 지지 백금에 있어서 일산화탄소의 흡착에 관한 연구

박상윤,윤구식,이재덕 東亞大學校附設基礎科學硏究所 1999 基礎科學硏究論文集 Vol.16 No.1

실리카 지지 백금에서 백금의 함량, 일산화탄소의 압력, 그리고 흡착온도의 변화에 따른 CO의 흡착 및 탈착거동에 대한 연구를 수행하였다. 선형결합한 일산화탄소에 의한 C-O 신축진동 적외선 흡수띠가 백금 함량이 4%인 경우 ??, 2%인 경우 ??, 1%인경우 ??, 다리 또는 다중결합한 일산화탄소 C-O신축진동 적외선 흡수띠는 ??, 계단자리 또는 모서리에 결합한 일산화탄소에 의한 C-O 신축진동 적외선 흡수띠는 ?? 나타났다. 일산화탄소의 압력을 변화시킬 때(0.2∼50torr), 위에 언급한 흡수띠들이 어느 정도 단파장 쪽으로 이동하고?? 백금 함량 4%의 흡수띠의 세기는 큰 변화가 없지만 백금 함량 1%와 2%의 경우는 커다란 변화가 있었다. 진공탈착시켰을 때 짧은 시간(1시간) 탈착시키면 흡수띠 세기는 큰 변화가 없지만 오랜 시간(12시간) 탈착시키면 그 세기는 현저하게 작아지고 백금 함량이 1%인 경우에는 흡수띠가 거의 사라졌다. 백금 함량 4% 시료에 대해 일정한 일산화탄소 압력(1.0torr)에서 흡착 온도를 변화시켰을 때 선형결합한 일산화탄소에 의한 C-O 신축진동 적외선 흡수띠가 약간 장파장으로 이동하며(?? 온도가 높아질수록 흡수띠의 나비가 점점 넓어졌다. 100℃ 이상부터 나타나는 이 흡수띠 어깨 ?? 실리카 흡수띠에 의하여 생성되는 것으로 볼 수 있다. We have investigated the infrared spectra for CO adsorbed on silica supported platinum at various CO partial pressures and various temperatures within the frequency range of ??. Due to the linear bonded CO, the ?? bands in 4 wt% Pt/SiO₂, the ?? bands in 2 wt% Pt/SiO₂, and the?? bands in 1 wt% Pt/SiO₂ were observed. Also the?? bands due to bridged or multiple bonded CO and the ?? bands due to CO adsorbed on step and edge site were observed. With increasing CO pressure all the above bands showed blue shifts and the intensities of the bands in 4 wt% Pt/SiO₂ increased a little but those in 2 wt% Pt/SiO₂ and in 1 wt% Pt/SiO₂ increased much within our experimental CO pressure range. With pumping for evacuation during 1 hour the intensities of the bands decreased a little but with pumping during 12 hour the intensities of the bands decreased much. Especially the bands in 1 wt% Pt/SiO₂. were almost disappeared. With increasing temperature of the 4 wt% Pt/SiO₂ sample at constant CO pressure(1.0torr) the linear adsorption band showed red shift ?? and the width of the band went broad. It was suggested that the newly appearing ?? shoulder of this band above 100℃ is attributed to th

Over-expression of a seed specific hevein-like antimicrobial peptide from Pharbitis nil enhances resistance to a fungal pathogen in transgenic tobacco plants

Koo, Ja-Choon,Chun, Hyun-Jin,Park, Hyeong-Cheol,Kim, Min-Chul,Koo, Yoon-Duck,Koo, Seong-Cheol,Ok, Hyun-Mi,Park, Soo-Jeong,Lee, Sung-Ho,Yun, Dae-Jin,Lim, Chae-Oh,Bahk, Jeong-Dong,Lee, Sang-Yeol,Cho, Mo Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated pnAMP-h1 and pnAMP-h2, and the corresponding genomic clones encoding these protein suggests that the peptides are produced as a prepropeptide consisting of and N-terminal signal peptide, the mature protein and C-terminal domains. The transcripts of the two genes are accumulated seed-specifically, and the maximum transcripts are observed in the mid-to-late stage of seed development. Constitutive over-expression of the pnAMP-h2 cDNA in transgenic tobacco under the control of the cauliflower mosaic virus 35S promoter conferred enhanced resistance against the oomycete Phytophthora parasitica, the causal agent of black shank disease. Thus the Pn-AMPs may play a role in the protection of seeds and may

이개 상후방 확장 절개를 가한 Zig-Zag 관상 절개법을 이용한 관골골절의 치료

윤을식,구상환,안덕선,박승하 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.1

The ideal surgical approach to treat craniomaxillofacial fractures should provide maximum exposure of the fractured segments, ensure less potential for injury to facial structures, and allow for good cosmetic results. The most frequently used approach is the coronal incision. However, the coronal approach poses some disadvantages. The most common complication is widening of the scar at the head. The inevitable scar, whether narrow or wide, produce a natural separation of the hair. Also, hairline recession may expose the scar above the level of the ear and preauricular sensory deficits and motor deficits involving the frontal branch of the facial nerve have been reported. In order to avoid above problems, we have used zig-zag coronal incision with a postauricular extension from September, 1996 to May, 1998 in 35 cases with diagnosis varying from zygoma complex fracture to extensive facial fracture. Clinical follow-up ranged from 2 to 22 months. In our series of 35 patients, one patient had hematoma, one patient transient weakness of the frontalis muscle and one patient had sensory deficit. We found that this approach maintains the advantages of lowering the pivot point but also lessens the disadvantages of an anterior incision and unlike the straight-line coronal incision, this incision line is immediately much less visible.

The tumorigenic, invasive and metastatic potential of epithelial and round subpopulations of the SW480 human colon cancer cell line.

Yoon, Wan-Hee,Lee, Sang-Kwang,Song, Kyoung-Sub,Kim, Jong-Seok,Kim, Tae-Dong,Li, Ge,Yun, Eun-Jin,Heo, Jun-Young,Jung, Yeon-Joo,Park, Jong-Il,Kweon, Gi-Ryang,Koo, Sun-Hoe,Park, Hae-Duck,Hwang, Byung-Doo D. A. Spandidos 2008 MOLECULAR MEDICINE REPORTS Vol.1 No.5

<P>It has been reported that the SW480 human colon cancer cell line consists of E-type and R-type cells. The long-term tumorigenic potential, invasive and metastatic properties of these subclones have not been characterized. E-type and R-type cells were subcloned using limiting dilution methods from parental SW480 cells. The cell growth rate was determined by MTT colorimetric assay, and colony forming efficiency was analyzed using Matrigel-coated plates. The activity of matrix metalloproteinase (MMP) and of urokinase plasminogen activator (uPA) was assessed by zymography. Invasive and locomotive ability was analyzed using transwell chambers. In situ apoptosis detection of these subclones was also performed. In vivo long-term tumorigenicity and nodal metastasis were evaluated using nude mice. E-type cells produced spontaneously regressive tumors in spite of invasion and lymph node metastasis. In contrast, R-type cells revealed progressively growing tumors without invasion or metastasis. E-type cells exhibited increased apoptosis and invasive and motile ability, as well as strong MMP-9 and -2 activity. Although phorbol 12-myristate 13-acetate treatment induced MMP-9 activity in E-type cells, it had no effect on R-type cells. These findings suggest that E- and R-type cells may have different biological properties in terms of colon cancer progression, regression, invasion and nodal metastasis, and might serve as a useful model for these studies.</P>

부안각 경결막 절개를 통한 안와 골절의 정복

안덕선,박승하,구상환,윤을식 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.3

Orbital fracture represents one of the common lesions encountered today with our modern mechanized life. Reduction of these fracture is important in preventing the untoward complications that may ensue such as diplopia and enopththalmos. Although various types of incision have been employed to approach the orbital floor and infraorbital rim, subcilliary and transconjunctival approach are most commonly used. The subcilliary approach has been used for a number of years successfully although it does have shortcommings such as cutaneous scar and ectropion in many cases. The conventional transconjunctival approach provides limited exposure, so it can be used only with small fractures. In order to avoid above problems, it is preferable to use a paracanthal transconjunctival incision: however, it is combined with severance of the lower limb of the lateral canthal tendon approximately 2mm from the canthus. We have used this technique in fifty one cases with diagnosis varying from blow-out fracture to extensive facial fracture. We found that this approach provides good exposure and gives an excellent postoperative result with a concealed apparent cutaneous scarring and no scleral or corneal complications and visualization of the orbital floor and rim with minimal morbidity and insignificant cosmetic impact. In contrast to previously reported similar approaches, it is simple to use in traumatic and congenital lesions that go beyond the orbital floor.

임상 병기에 따른 췌장암의 생존 기간과 예후 인자

김윤지 ( Yoon Ji Kim ),서동완 ( Dong Wan Seo ),백관미 ( Kwan Mi Pack ),정은희 ( Eun Heui Jeong ),김송철 ( Song Cheol Kim ),한덕종 ( Duck Jong Han ),이상수 ( Sang Soo Lee ),이성구 ( Sung Koo Lee ),김명환 ( Myung Hwan Kim ) 대한소화기학회 2008 대한소화기학회지 Vol.51 No.3

목적: 췌장암은 예후가 극히 불량하며, 점차 발생빈도가 증가하고 있다. 췌장암의 예후에 영향을 미치는 인자에 대한 많은 연구 결과가 보고되었다. 그러나, 각기 다른 환자, 기와 치료 방법을 포함하고 있어 일정한 결론에 이르지 못하였다. 이에 저자 등은 대규모 연구를 통해 췌장암의 생존기간에 영향을 주는 예후 인자들을 분석하여 향후 치료의 방향을 결정하는 데 도움이 되고자 하였다. 대상 및 방법: 1994년 1월부터 2004년 1월까지 서울아산병원에서 췌장암으로 진단되어 치료를 받았던 971명의 환자를 대상으로 하였다. 환자들은 임상 병기에 따라 절제 가능형(226명), 국소 진행형(409명), 말기 진행형(336명)의 세 군으로 나누었다. 후향 조사를 통하여 각 군 간의 생존기간과 이에 영향을 주는 인자에 대해 분석하였다. 또한 치료 방법에 따른 생존기간을 비교하여 췌장암의 적극적인 치료가 생존율의 향상에 도움을 주는지 알아보았다. 결과: 적극적인 치료를 한 환자와 보존 치료를 받은 환자의 중앙 생존기간은 절제 가능형에서 각각 18개월과 9개월, 국소 진행형에서 각각 10개월과 7개월, 말기 진행형에서 5개월과 3개월로, 모든 병기에서 적극적인 치료를 받은 환자들의 생존기간이 길었다. 다변량 분석 결과, 절제 가능형에서는 CA 19-9 농도, 조직 분화도, 종양의 크기, 국소 림프절 전이 여부가 예후에 영향을 주는 독립적인 인자로 나타났다. 반면에 말기 진행형에서는 연령, 수행 능력, 알부민 농도가 예후와 관련된 인자로 확인되었다. 결론: 췌장암의 적극적인 치료는 진행된 병기를 가진 환자에서도 생존기간을 연장시킬 수 있었다. 또한 췌장암의 예후와 관련된 인자는 임상 병기에 따라 차이를 보여, 절제 가능형 췌장암에서는 주로 종양의 특성과 관련된 인자가, 반면에 말기 진행형 췌장암에서는 주로 환자의 특성과 관련된 인자가 생존율에 영향을 주었다. Background/Aims: The prognosis of patients with pancreatic cancer remains very poor. Although many studies have evaluated the prognostic factors of pancreatic cancer, their results are inconclusive because of different inclusion criteria, tumor stages, and treatment modalities. This large scale retrospective analysis was performed to assess whether active treatment of pancreatic cancer, even in its advanced stage, could improve patients` survival. In addition, we sought to identify factors associated with favorable prognosis of pancreatic cancer. Methods: Between 1994 and 2004, a total of 971 patients with pancreatic cancer were treated at Asan Medical Center. The patients were classified into three groups according to clinical stages: resectable (RE, n=226), locally advanced (LA, n=409), and far advanced (FA, n=336). Treatment response and prognostic factors for survival were analyzed in each group. Results: Compared to supportive care, active treatment significantly increased the median survival time in all groups (RE: 18.0 vs. 9.0 months; LA: 10.0 vs. 7.0 months; FA: 5.0 vs. 3.0 months). Multivariate analysis showed that prognostic factors for survival differed according to clinical stages. In the RE group, unfavorable prognostic factors were high CA 19-9, poor histologic differentiation, large tumor size, and regional lymph node involvement. In the FA group, however, poor outcomes were associated with old age, poor performance status, and hypoalbuminemia. Conclusions: More active treatment of pancreatic cancer, even in advanced stage, can make a significant difference in terms of patient`s survival. The prognosis of resectable pancreatic cancer is dependent on tumor-related factors, while the prognosis of patients with far advanced pancreatic cancer is dependent on patient-related factors. (Korean J Gastroenterol 2008;51:181-189)

요천추부 X-선상 이상소견과 요통 발생의 상관적 연구

윤형구(Yoon Hyung Koo),박준식(Park Joon Sik),조덕연(Cho Duck Yun),김기용(Kim Key Yong) 대한정형외과학회 1978 대한정형외과학회지 Vol.13 No.4

한단계 이개전방 피판을 이용한 남성형 탈모증의 치료

안덕선,윤을식,구상환,박승하 大韓成形外科學會 1996 Archives of Plastic Surgery Vol.23 No.6

Traditionally, the methods of treating male pattern baldness has been classified as hair graft, scalp transposition flap and scalp reduction. The most effective method of treating baldness in the frontal region in the flap technique, which is ideal for getting a sufficial amount of hair to cover an extensive area of baldness at one time. Since the first description by Dardour in 1983, the one-stage preauricular flap has shown the excellent result in the treatment of male pattern baldness. In this paper, the authors used One-stage Preauricular flaps to treat 15 frontal baldness patients with various causes from January, 1995 to April 1996. This method is suitable for Types II and III baldness, which, according to Hamilton classification, is a mild baldness where the hairline retreats in the front and the temporal region is a triangle. Its advantages include high hair density, anterior direction of hair growth, the fact that it can be done in a single stage under local anesthesia, and finally, an aesthetic result characterized by a pleasing frontal hairline with natural temporal recess. This surgical technique is not frequently used when reconstructing frontal hairline but it can bring satisfactory aesthetic results if conducted on the cautiously selected patients under the age of 40 who are non-smokers with good skin elasticity. The technique can also be applied primarily in reconstructing frontal hairlines of male pattern baldness patients belonging to other types of Hamilton classification. Adjuvant micrografting refined the frontal hairline for the most natural-appearing result.

말초신경 손상에 의한 배근신경절 세포의 전기생리적 특성 변화

장성구 ( Sung Koo Chang ),정승수 ( Seung Soo Chung ),임중우 ( Joong Woo Leem ),은정우 ( Jung Woo Eun ),윤덕미 ( Duck Mi Yoon ),남택상 ( Taick Sang Nam ) 대한통증학회 2003 The Korean Journal of Pain Vol.16 No.1