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Synthesis Backbone-Dual-Responsive of Hyperbranched Poly(bis(N,N-ethyl acrylamide))s By RAFT
Yonggang Wu,Libin Bai,Guang Li,Wenliang Li,Sujuan Wang,Xinwu Ba,Guoqiang Zhou,Hongchi Zhao 한국고분자학회 2014 Macromolecular Research Vol.22 No.11
Hyperbranched poly(bis(N,N-ethyl acrylamide))s (HPNAMs) with many vinyls as terminal groups weresynthesized successfully using reversible addition fragmentation chain transfer polymerization (RAFT). Detailedanalyses, based on the molecular weight, α value, degree of branching (DB), and lower critical solution temperature (LCST)obtained from nuclear magnetic resonance (NMR), multi detector-size exclusion chromatography (MDSEC), ultravioletvisible(UV-vis) spectroscopy, and dynamic light scattering (DLS), indicate that we acquired backbone-temperatureand pH responsive HPNAM. Factors, such as DB, molecular weight, and pH value, that affect the LCST were investigated. It was found that the molecular weights of the hyperbranched polymers show significant influence on the LCSTs. For the HPNAMs with low molecular weight, the LCSTs decreased as the DB increased, and the LCSTs can also beadjusted by changing the pH value of solutions. Furthermore, the result of cell cytotoxicity indicates that this new dualresponsive hyperbranched polymer has low cytotoxicity and exhibits potential for biomedical applications.
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Haisong Zhang,Tianci Ren,Meng Yu,Hailei Zhang,Libin Bai,Yonggang Wu,Sujuan Wang,Xinwu Ba 한국고분자학회 2016 Macromolecular Research Vol.24 No.8
Curcumin is the physiologically and pharmacologically active component of turmeric (Curcuma longa L.) with attractive fluorescent property. However, the applications in live cell imaging field are restricted mainly due to the water-insolubility and cytotoxicity. To overcome this problem, curcumin-incorporated glycopolymers are synthesized by free radical copolymerization in this study. The glycopolymers are well-characterized by infrared spectroscopy (IR), nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), UV-Vis absorption spectroscopy and photoluminescence spectroscopy. Furthermore, obtained glycopolymers are assayed by MTT assay and cell imaging for supporting the potential bio application. The MTT result indicates that the introduction of carbohydrate units can reduce the cytotoxicity of curcumin unit. The live cell imaging investigations reveal that the curcuminincorporated glycopolymers are good candidates for cell imaging and may find broad applications in biological areas such as biological diagnosis, imaging, and detection.
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