The Burholderia pyrrocinia Purple Acid Phosphatase Pap9 Mediates Phosphate Acquisition in Plants

Xiaoli Zhu,이승엽,양원태,이선우,백동원,Ming Shun Li,김도훈 한국식물학회 2019 Journal of Plant Biology Vol.62 No.5

Increasing the expression level of purple acidphosphatases (PAPs), which hydrolyze organic phosphate toinorganic phosphate (Pi), is an important response to Pi starvationin plants. PAPs are widely distributed in eukaryotes likemammals and plants but distributed in limited microorganisms. A PAP-like protein named Pap9 from Burkholderia pyrrociniaCH-67 was isolated. The enzyme had optimal activity atatypical pH of 8.5 and temperature of 85°C. When pap9 wasoverexpressed in Arabidopsis thaliana, the APase activity inthe transgenic plants was about 15% to 40% higher underacidic conditions and had an approximately four-fold increaseunder alkaline conditions compared with wild type. Overexpression of pap9 in plants enhanced APase activityunder Pi-deficient conditions more than under Pi-sufficientconditions. In-gel assays revealed that Pap9 exists as amonomer in transgenic plants. Root surface-associatedAPase activity in transgenic plants increased dramaticallyduring acidic and Pi-deficient conditions. These resultsindicated that bacterial Pap9 in transgenic plants wassecreted onto the root surface and released into the rhizosphere. The transgenic plants have significantly enhanced biomassthat have the potential for use in biotechnologicalapplications.

Scheduling with Heterogeneous QoS Provisioning for Indoor Visible-light Communication

Xiaoli Dong,Xuefen Chi,Hongliang Sun,Yuhong Zhu 한국광학회 2018 Current Optics and Photonics Vol.2 No.1

Visible-light communication (VLC) combined with advanced illumination can be expected to become an integral part of next-generation communication networks. One of the major concerns in VLC implementation is developing resource-allocation schemes in a multi-user scenario. However, the scheduling for heterogeneous quality of service (QoS) traffic has not been studied so far, for the indoor VLC downlink system. In this paper, we creatively introduce effective-bandwidth and effective-capacity theory into the multi-user scheduling (MUS) problem, to guarantee the user’s statistical delay QoS. We also take account of the aggregate interference (AI) in the indoor VLC downlink system, and analyze its impact on the user-centric MUS problem for the first time. Simulations show that the AI has a nonnegligible influence on the scheduling result, and that the proposed scheduling scheme could guarantee the user’s QoS requirement under the premise of ensuring sum capacity.

Expressing a Modified Cowpea Trypsin Inhibitor Gene to Increase Insect Tolerance Against Pieris rapae in Chinese Cabbage

Xiaoli Ma,Zhen Zhu,Yane Li,Guangdong Yang,Yanxi Pei 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.2

To increase the tolerance of Chinese cabbage (Brassica campestris L. ssp. pekinensis) to Pieris rapae, weintroduced a modified cowpea trypsin inhibitor (CpTI) gene, SCK, into various cultivars. SCK was derived fromCpTI, an insect-resistance gene. The protein accumulating capacity of CpTI can be improved by adding a signalpeptide sequence at the 5' end and an endoplasmic reticulum-detained signal sequence at the 3' end. Using an optimizedAgrobacterium tumefaciens-mediated transformation system in Chinese cabbage, we obtained a maximum transformationefficiency of ~6.83%. Insect resistance tests and CpTI enzymatic assays showed that most of the transgenic plantshad significant resistance to cabbage worm (Pieris rapae) larvae and that the plants with the highest levels of insectresistance had the greatest CpTI-related capacity, indicating a high correlation between SCK expression and insectresistance. An evaluation of segregation patterns in the independent transgenic line with the highest insect resistance,‘ZB-08-04’, showed that kanamycin resistant versus sensitive plants segregated in a 3:1 Mendelian fashion. This studyprovides a potential germplasm resource for Chinese cabbage breeding in the future.

Prediction of the Amount of Soil Discharged by an Earth Pressure Balanced Shield Machine Based on Feature Engineering

Zhu Wen,Zhen Wang,Xuening Rong,Xiaoli Rong,Ziming Xiong,Baohuai Yang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.12

The ground loss caused by earth pressure balance (EPB) shield tunneling is a serious threat to the safety of ground and underground buildings. The amount of soil discharged is a direct factor causing the ground loss, but the feedback of the amount of soil discharged has the problems of human subjectivity and information lag. Based on the theory of soil discharged and shield construction data, a data model for predicting the amount of soil discharged is constructed, and a predictive method of soil discharged based on adaptive adjustment strategy and parameters to achieve optimal regression is proposed. Taking Changzhou shield tunnel construction project as an example, through comparative analysis, the main influencing factors of soil discharge data model are screw-conveyor torque, screw-conveyor revolutions, total thrust, screw-conveyor entrance earth pressure, the revised standard penetration test blow-count, and compression modulus. The results show that the mean absolute error (MAE) of the model is 0.911 m3, and the mean absolute percentage error (MAPE) is 2.78%, which verifies the effectiveness of the prediction of soil discharged by feature engineering.

Comparison of the Nutritional Composition of Bullfrog Meat from Different Parts of the Animal

Yanli Zhu,Min Bao,Chong Chen,Xiaoli Yang,Wenliang Yan,Fazheng Ren,Pengjie Wang,Pengcheng Wen 한국축산식품학회 2021 한국축산식품학회지 Vol.41 No.6

The objective of this study was to evaluate the nutritional content of bullfrog meat from different parts of the animal, including fore-chest, thigh and calf. Bullfrog meat was found to be a rich source of proteins, essential amino acids and minerals, but with a low fat content, compared with other aquatic meat products. There was no significant difference (p>0.05) between thigh and calf in mineral content (K, P, Na, Mg, Ca, Zn, Fe, Cu, and Mn), but the contents of K, P, and Mg were higher in thigh and calf than in the fore-chest (p<0.05). The salt-soluble, water-soluble and insoluble protein bands in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis, from fore-chest, thigh and calf were similar, with the most abundant bands being 35 kDa (salt-soluble protein), 35-48 kDa (water-soluble protein) and 48 kDa (insoluble protein). The results showed that the insoluble protein content in the fore-chest meat was higher than that in the thigh meat and calf meat, but the salt-soluble protein fraction was the most abundant in thigh meat. These results showed that the nutrients in different parts of bullfrog meat were different.

20(S)-ginsenoside Rh2 induces caspase-dependent promyelocytic leukemia-retinoic acid receptor A degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades

Sirui Zhu,Xiaoli Liu,Mei Xue,Yu Li,Danhong Cai,Shijun Wang,Liang Zhang 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2

Background: Acute promyelocytic leukemia (APL) is a hematopoietic malignancy driven by promyelocytic leukemia―retinoic acid receptor A (PML-RARA) fusion gene. The therapeutic drugs currently used to treat APL have adverse effects. 20(S)-ginsenoside Rh2 (GRh2) is an anticancer medicine with high effectiveness and low toxicity. However, the underlying anticancer mechanisms of GRh2-induced PML-RARA degradation and apoptosis in human APL cell line (NB4 cells) remain unclear. Methods: Apoptosis-related indicators and PML-RARA expression were determined to investigate the effect of GRh2 on NB4 cells. Z-VAD-FMK, LY294002, and C 87, as inhibitors of caspase, and the phosphatidylinositol 3-kinase (PI3K) and tumor necrosis factor-α (TNF-α ) pathways were used to clarify the relationship between GRh2-induced apoptosis and PML-RARA degradation. Results: GRh2 dose- and time-dependently decreased NB4 cell viability. GRh2-induced apoptosis, cell cycle arrest, and caspase3, caspase8, and caspase9 activation in NB4 cells after a 12-hour treatment. GRh2-induced apoptosis in NB4 cells was accompanied by massive production of reactive oxygen species, mitochondrial damage and upregulated Bax/Bcl-2 expression. GRh2 also induced PML/PML-RARA degradation, PML nuclear bodies formation, and activation of the downstream p53 pathway in NB4 cells. Z-VAD-FMK inhibited caspase activation and significantly reversed GRh2-induced apoptosis and PML-RARA degradation. GRh2 also upregulated TNF-a expression and inhibited Akt phosphorylation. LY294002, an inhibitor of the PI3K pathway, enhanced the antitumor effects of GRh2, and C 87, an inhibitor of the TNF-α pathway, reversed NB4 cell viability, and GRh2-mediated apoptosis in a caspase-8-dependent manner. Conclusion: GRh2 induced caspase-dependent PML-RARA degradation and apoptosis in NB4 cells via the Akt/Bax/caspase9 and TNF-α/caspase8 pathways.

Study on the Vertical Dynamic Coupled Effects of the Crowd-structure System based on the Social Force Model

Qiankun Zhu,Xiaoli Hui,Nana Nan,Yongfeng Du 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.5

Due to the complexity and randomness of the crowd motion, it is not easy to establish the crowd-structure coupled governing equation. Therefore, the idea of the solution procedure for the coupled system of the single pedestrian and structure is extended to carry out the research of crowd-structure coupled system. Then based on the social force model and the deceleration mechanism aswell as the self-stopped mechanism, the one-way pedestrian flow is simulated. The modified Differential Quadrature-Integral Quadrature (DQ-IQ) mixed method is used to solve the dynamic response of the structure under the moving stiffness-mass-damping model, which can demonstrate this method enjoys higher accuracy than the mode superposition method and can take enough higher modes into consideration. Additionally, the fact that the DQ-IQ mixed method can be used to solve the dynamic response of the structure subjected to the crowd is verified. The final results under random crowd show that the varying tendencies of structural modal parameters respectively changed with the the number of pedestrian acting on the structure, In order to more comprehensively and more scientifically measure the vibration function of the structure, the 1 s-RMS acceleration is introduced to weaken the interference effect of random fluctuation.

A full path assessment approach for vibration serviceability and vibration control of footbridges

Qiankun Zhu,Xiaoli Hui,Yongfeng Du,Qiong Zhang 국제구조공학회 2019 Structural Engineering and Mechanics, An Int'l Jou Vol.70 No.6

Most of the existing evaluation criteria of vibration serviceability rely on the peak acceleration of the structure rather than that of the people keeping their own body unmoved on the structure who is the real receiver of structural vibrations. In order to accurately assess the vibration serviceability, therefore, a full path assessment approach of vibration serviceability based on vibration source, path and receiver is not only tentatively proposed in this paper, taking the peak acceleration of receiver into account, but also introduce a probability procedure to provide more instructive information instead of a single value. In fact, semi-rigid supported on both sides of the structure is more consistent with the actual situation than simply supported or clamped due to the application of the prefabricated footbridge structures. So, the footbridge is regarded as a beam with semi-rigid supported on both sides in this paper. The differential quadrature-integral quadrature coupled method is not only to handle different type of boundary conditions, but also after being further modified via the introduction of an approximation procedure in this work, the time-varying system problem caused by human-structure interaction can be solved well. The analytical results of numerical simulations demonstrate that the modified differential quadrature-integral quadrature coupled method has higher reliability and accuracy compared with the mode superposition method. What’s more, both of the two different passive control measures, the tuned mass damper and semi-rigid supported, have good performance for reducing vibrations. Most importantly, semi-rigid supported is easier to achieve the objective of reducing vibration compared with tuned mass damper in design stage of structure.

Ultrasonic Synthesis of Au/AgCl Hybrid Cubes and Their Evolution Under Electron Beam Irradiation

Hongliang Cao,Xiaoli Miao,Mengqin Zhu,Chang Li,Feifei Wei,Xin Chen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.6

The utilization of ultrasound offers a facile tool for the synthesis of Au/AgCl hybrid particles. We have obtained the architectures of Au nanoparticles on AgCl sub-micrometer cubes by simply ultrasonic irradiating their precursors in ethylene glycol (EG). The formation process of Au/AgCl hybrid particles in the reaction solution has been studied based on the optical absorption spectra and the scanning electron microscope (SEM) images of the particles obtained at different ultrasonic irradiation stages. The dynamic changes of Au/AgCl hybrid particles under electron beam irradiation have been investigated in both SEM and in situ liquid cell transmission electron microscope (TEM). Hollow Au/AgCl hybrid particle structures have been obtained under the electron beam irradiation in liquid, with the cuboid contour shape preserved.

AMPK alleviates high uric acid-induced Na+-K+-ATPase signaling impairment and cell injury in renal tubules

Jing Xiao,Sibo Zhu,Haochen Guan,Haochen Guan,Fengqin Li,Xiaoli Zhang,Hui Guo,Xiaojun Wang,Zhibin Ye 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

One of the mechanisms in hyperuricemia (HUA)-induced renal tubular injury is the impairment of Na+-K+-ATPase (NKA) signaling, which further triggers inflammation, autophagy, and mitochondrial dysfunction and leads to cell injury. Here, we used RNA sequencing to screen the most likely regulators of NKA signaling and found that the liver kinase B1(LKB1)/adenosine monophosphate (AMP)-activated protein kinase (AMPK)/ mammalian target of rapamycin (mTOR) pathway was the most abundantly enriched pathway in HUA. AMPK is a key regulator of cell energy metabolism; hence, we examined the effect of AMPK on HUA-induced dysregulation of NKA signaling and cell injury. We first detected AMPK activation in high uric acid (UA)-stimulated proximal tubular epithelial cells (PTECs). We further found that sustained treatment with the AMPK activator 5-aminoimidazole-4-carboxamide 1-β-d-ribofuranoside (AICAR), but not the AMPK inhibitor Compound C, significantly alleviated UA-induced reductions in NKA activity and NKA α1 subunit expression on the cell membrane by reducing NKA degradation in lysosomes; sustained AICAR treatment also significantly alleviated activation of the NKA downstream molecules Src and interleukin-1β (IL-1β) in PTECs. AICAR further alleviated high UA-induced apoptosis, autophagy, and mitochondrial dysfunction. Although AMPK activation by metformin did not reduce serum UA levels in hyperuricemic rats, it significantly alleviated HUAinduced renal tubular injury and NKA signaling impairment in vivo with effects similar to those of febuxostat. Our study suggests that AMPK activation may temporarily compensate for HUA-induced renal injury. Sustained AMPK activation could reduce lysosomal NKA degradation and maintain NKA function, thus alleviating NKA downstream inflammation and protecting tubular cells from high UA-induced renal tubular injury.