Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

韓國産 자작나무科의 系統分類學的 硏究(序論)

李相泰,蘇雄永,田寬培,李愚喆 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-

The present paper is an introduction to the whole project of "The systematic studies on Korean Betulaceae". Three studies under the project were performed this year and presented in this report : a review of taxonomic literatures and the construction of distribution maps by Woo-Tchul Lee, pollen morphology by Sang-Tae Lee and Moo-Yeol Kim, and wood anatomy by Woong-Young Soh and Kwan-Bae Jeon.

자연산 점몰개, Squalidus multimaculatus Hosoya et Jeon의 새인두흡충, Clinostomum complanatum (Rudolphi, 1814) 감염

박명애 ( Myoung Ae Park ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),최희정 ( Hee Jung Choi ),전은지 ( Eun Ji Jeon ),지보영 ( Bo Young Jee ),이완옥 ( Wan-ok Lee ),우승호 ( Sung Ho Woo ),이은혜 ( Eun Hye Lee ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2

The metacercariae of Clinostomum complanatum (Rudolphi, 1814) were isolated in the whole body muscle of wild freshwater fish, spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon) from the local river in Yeongdeok, Gyeongsangbuk-do, Korea. The infected fish with bumpy body surface by yellow grub showed erratic swimming behavior and some fish were died. The isolated excysted metacercariae were tongue shaped and 2.85~5.89 mm × 0.8~1.99 mm in size. 1~98 metacercariae were isolated in individual fish and mean infection intensity was 25. All examined adult spotted barbel gudgeon sizing 7 cm in body length were infected. This is the first report of disease outbreak and mortality caused by C. complanatum infection in wild freshwater fish in Korea.

탈북자들의 신앙 경험과 교회의 통일 준비

전우택,조영아 연세대학교 통일연구원 2003 통일연구 Vol.7 No.2

This study was conducted to investigate the religious experiences of North Korean defectors in South Korea and the preparation of the Church for the Korean unification. Twenty-three North Korean defectors were interviewed qualitatively, and the data from the interview were analyzed using the theme analysis method. Results showed that the reasons why North Korean defectors held a religion in South Korea were as follows: (1) to receive help for overcoming life difficulties (2) to maintain relationship with the people who helped the defectors when they entered South Korea (3) to broaden personal connections (4) to receive financial support and (5) to be guided by moral aspects of the religion. On the other hand, North Korean defectors reported the reasons why they had difficulties in keeping religion and why they abandon ed it as follows: (1) unfamiliarity with religion (2) religion posing aspects that were similar to Ju-Che ideology, which were educated in North Korea (3) negative experiences in the church and (4) their absolute judgemental attitude. Theses results suggests ways that South Korean churches could prepare for Korean unification as follows: (1) Churches should try to understand the psychological and cognitive characteristics of North Korean people and North Korean defectors (2) need to develop religion education programs for North Korean defectors (3) need to confirm the basic doctrine of Christianity in preparing unification and lastly (4) need to be more active, balanced, and initiative i n the process of unification.

철근콘크리트 구조물의 균열발생 현황 및 원인 조사연구

전광우,정재동,최영화 대구대학교 과학기술연구소 1997 科學技術硏究 Vol.4 No.3

The purpose of this study is to propose the pertinent plan for crack through analyzing types, styles, periods, parts and features of crack for national concrete-related pursuers in domestic working site. And this is a basic study on control of crack which is presented to reinforced concrete structure. For these purposes, as we make check-list about each cause of crack and eliminate the cause of crack in first place in job site, we can make an effective method of control the crack as a whole.

체중감량이 뇨전해질, 일반혈액성분에 미치는 영향

전승훈,김창근,권영우 한국체육대학교 2001 論文集 Vol.24 No.-

The purpose of this study to investigated the effects of wrestling' weight loss on urine electrolyte (Na+, K+), Blood cell count, heart rate. Twenty one subjects participated in this study and reduced approximately 7% per body weight. The subject was divided short period weight reduction (n=6) during 7day, long period weight reduction (n=6) during 14day. Urine sample extracted 20 ml for electrolyte analysis from pre-, post body weight reduction, recovery(1day later). Similarly Blood cell count measured. For measuring heart rate, ubject performed anaerobic power test during 30 second, measuring immediately, 3, 6, 9. 15 minute' recovery period. The result following: Short terms weight reduction group showed no significant different in body composition (LBM and fat %) but long terms weight reduction group was significantly reduced lean body mass after weight reduction but did not change fat. The content of urine Na+ and K+ decreased significantly after body weight reduction in both group. In recovery period, urine K+ did not show any variation in both group. Urine Na+ returned to base line during recovery period in long term body weight reduction group, but these changes was not statistically significant. In conclusion, weight reduction of wrestling caused the change body composition, electrolyte and short terms weight reduction may detrimental effects body' homeostasis.

오존노출이 실험적으로 유발된 알레르기성 비염에 미치는 영향

전우진,변상내,김용민,김병국,나기상,박찬일 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.11

정상 인체피부섬유모세포종에 대한 벤조산의 독성효과

전주원,손영우,서부일,한두석 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objective : The aim of this study was to evaluate cytotoxic effect of benzoic acid on normal human skin fibroblast cell line , Detroit 551. Methods : The cytotoxicity was determined by colorimetric assays such as MTT assay or XTT assay. The microscopy was carried out to observe Detroit 551 cell line. Cells were incubated with various concentrations of benzoic acid in CQ incubator controlled with 37 c for 48 hours. Results : The cytotoxic effect of benzoic acid on Detroit 551 did not show a positive significance at 50 uM and 100 uM benzoic acid by MTT assay or X T T assay compared with control. MTTB and XTTg were 900.6 uM and 974.6 uM, respectively. In microscopic study, Detroit 551 treated with benzoic acid showed a slightly decreased cell number, while the morphological changes of cell was not showed. Conclusion : Above the results, the cytotoxicity of benzoic acid was midtoxicity on normal human skin fibroblasts cell line. Detrit 551

CT flouroscopy를 이용한 중재적 시술의 유용성

전혜정,박정희,서보경,박상우,신현준,최영철,한혜승,이창희 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Conventional CT-guided needle localization has been reported many times, but a few reports of interventional procedures by using multidetector CT scan with CT-fluoroscopy have been described. We presented 36 cases of CT-fluoroscopy guided interventional procedures focusing on the puncture technique. Two cases of small pneumothorax and one case of pulmonary hemorrhage out of 2lcases after lung biopsy, were not needed any treatments We can easily and accurately access to the lesion by using CT-fluoroscopy and unique our marker system.

국어 교육과 화법

전영우 청람어문교육학회 2003 청람어문교육 Vol.27 No.-

화법연구는 다양한 관점에서 연구되어 왔다. 초기부터 현재까지 중요한 연구의 관점은 스피치의 구조, 내용, 문제에 관심을 가지는 수사학적 관점이다. 19세기에는 스피치 방법에 역점을 둔 기술적 관점이 제기되었고 현대에 오면서 심리학적, 임상적, 음성학적 접근이 화법연구의 중요한 경향으로 대두되었다. 최근에는 언어표현이 구체화된 발화현장에서 어떻게 기능하는가를 분석하는 화용론적 접근이 떠오르고 있다. 현대의 언어생활에서 언어생활은 인간관계에 중요한 영향을 미친다. 일반의미론적 관점에서 언어는 사물을 대신 하는 것 뿐이기 때문에 언어의 의미를 오해하거나 잘못 해석하기 쉽다. 따라서 화자가 의미하는 내용을 질문하고 메시지를 확인하여 가능한 한 정확한 정보를 입수한 뒤에 판단하고 반응으로 옮기는 것이 올바른 인간관계 커뮤니케이션의 요체이다. 화자는 부정확한 표현으로 상대에게 오해와 착각을 일으키지 말고 정확한 표현을 사용해야 한다. 정확한 표현은 사실과 추량의 구별, 시간과 장소가 없는 진술에 주의, 6하원칙에 의한 구체적 지적, 말하는 범위의 설정, 판단 척도의 눈금 사용 등의 원칙을 지켜야 한다. 또한 정확한 메시지 전달과 온전한 의사소통을 위해 말하기 듣기의 10단계 과정에서 발생하는 청자의 왜곡 현상과 불완전 커뮤니케이션 현상에 관심을 가져야 한다. Speech literature has been studied in various aspects. From the beginning to the present, the important aspect of the studies is the rhetorical approach which focus on the structure, content, style of the speech. The technological aspect which focus on the speech method was in vogue in 19th century, and psychological, rhetorical, clinical approach became the main trend of the modern speech literature. Lately, the speech act approach which analyze how speech expression function in concrete actual speech spot is emphasized. In modern life, speech life impacts an important influence on human relation. In aspect of the semantic approach, the meaning of the language is easy to misunderstand or misinterpret because language is just substitution of the object. Therefore, it is the main point of the right human relation to ask what the teller mean, confirm the message, input the precise information, interpret, and react.