A Novel Algorithm for Green Citrus Detection based on the Reticulate Grayladder Feature

Mingjun Wang,Jun Zhou,Weiyan Shang,Rufu Hu,Xuefeng Wang,Liang Gong 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10

Immature green citrus fruit detection using conventional color images is a challenging task due to fruit color similarity with the background, partial occlusion, varying illumination and shape irregularity. Therefore, most existing green fruits detection algorithms, which use color as the main discriminant feature, have a low recognition rate and a high rate of false positives. In this manuscript, we developed a novel Green Citrus fruit Detection algorithm based on the proposed Reticulate Grayladder Feature (GCDRGF), which contained 4 major steps: First, an 8-graylevel image was generated by the preprocessing steps of median filtering, histogram-based equalization and 8-graylevel discretization of the input raw image. Secondly, reticulate grayladders were obtained by a multidirectional scanning on the 8-graylevel image, and rule-based pseudo-grayladder removal strategies were used to remove false positives of target grayladders. Thirdly, grayladder clustering and fruit location fitting were used to generate candidate regions for target fruits. Finally, majority voting was performed to determine the results of candidate regions based on the analysis of apparent features and recticulate grayladders within candidate regions. The experimental results proved the effectiveness of the proposed reticulate grayladder feature and the corresponding detection algorithm with respect to various illuminant and imaging conditions. Compared with the existed eigenfruit algorithm, our algorithm has a higher rate of successful recognition and a lower rate of false positives, which helps to greatly improve the productivity of robotic operations.

Synthesis and Photo-Response of CuS Thin Films by an In Situ Multi-Deposition Process at Room Temperature: A Facile and Eco-Friendly Approach

Weiyan Liu,Huiming Ji,Jian Wang,Xuerong Zheng,Junyun Lai,Junna Ji,Tongfei Li,Yuanliang Ma,Haiqin Li,Suqin Zhao,Zhengguo Jin 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.3

"Uniform hexagonal covellite CuS thin films were deposited at room temperature by an in situ solution chemical reaction using copper precursor solid films as cationic source and ammonium sulfide ethanol solution as anionic reaction medium. We investigated the influence of both ethanolamine and butanol contents used in copper nitrate/ethylene glycol monomethylether (EGME) cationic solution for the preparation of copper precursor solid films, deposition cycle numbers and annealing treatment of the as-grown thin films by X-ray diffraction (XRD), FESEM, energy dispersive X-ray fluorescence EDX, transmission electron microscopy–Selected area electronic diffraction (TEM–SAED), atomic force microscopy (AFM) and ultraviolet-visible-near-infrared (UV-Vis–NIR) measurements. Photo-response of the CuS thin films was characterized by linear sweep voltammetry. The deposited CuS thin films were used to sensitize TiO2 anodes for solar cell application. The results showed that the CuS films had two-dimension oriented, half-sheet shaped growing morphology standing disorderly but vertically to substrates, and the calculated texture coefficient TC(102) verified that the half-sheet shaped crystallites had (102) plane orientation. This in situ multi-deposition process had an average deposited rate of 9 nm per cycle, and a selfperfect function to grow smooth, uniform and 2D oriented morphology with increase in the dip-cycle numbers. The photocurrent density was 14.5 Ma/cm2 at 1 V versus Ag/AgCl electrode for the annealed CuS thin films. CuS-sensitized TiO2 solar cells had a maximum conversion efficiency of 0.224%."

Research on Thermal Refocusing System of High-resolution Space Camera

Weiyan Li,Qunbo Lv,Jian Wei Wang,Na Zhao,Zheng Tan,Linlin Pei 한국광학회 2022 Current Optics and Photonics Vol.6 No.1

A high-resolution camera is a precise optical system. Its vibrations during transportation and launch, together with changes in temperature and gravity field in orbit, lead to different degrees of defocus of the camera. Thermal refocusing is one of the solutions to the problems related to in-orbit defocusing, but there are few relevant thermal refocusing mathematical models for systematic analysis and research. Therefore, to further research thermal refocusing systems by using the development of a high-resolution micro-nano satellite (CX6-02) super-resolution camera as an example, we established a thermal refocusing mathematical model based on the thermal elasticity theory on the basis of the secondary mirror position. The detailed design of the thermal refocusing system was carried out under the guidance of the mathematical model. Through optical–mechanical–thermal integration analysis and Zernike polynomial calculation, we found that the data error obtained was about 1%, and deformation in the secondary mirror surface conformed to the optical index, indicating the accuracy and reliability of the thermal refocusing mathematical model. In the final ground test, the thermal vacuum experimental verification data and in-orbit imaging results showed that the thermal refocusing system is consistent with the experimental data, and the performance is stable, which provides theoretical and technical support for the future development of a thermal refocusing space camera.

High-Conductivity SiO2-Matrix B-Doped Si-NC Thin Films by Following Ion-Beam Treatment

Junjun Huang,Weiyan Wang,Jie Yang,Yongzhen Tan,Wei Chen,Tianyu Ge,Yajun Zhang,Min Gao,Zhenming Chen 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.6

In this work, further ion-beam was performed on SiO2-matrix B-doped Si-NC (SBC) thin films in order to enhance conductivity. The effect of ionbeamtype on the electrical properties of SBC thin films was investigatedsystematically. The results indicated that the conductivities of SBC thinfilms were significantly improved by both argon and hydrogen ion-beamtreatments, and the higher the hydrogen ion ratio, the higher theconductivity of SBC thin films. The conductivity of SBC thin films wasincreased from 1.82 × 10−6 S/cm to 3.2 × 10−3 S/cm with followinghydrogen-ion-beam treatment. The change in conductivity of SBC thinfilms was most possibly resultant from the ion-beam treatment facilitatingthe formation of higher superficial order and lower defects. An alternativemethod was proposed to prepare high-conductivity SBC thin films, whichmay be applied to other heterogeneous thin films.

An Accurate Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry Method for Serum C-Peptide and Its Use in Harmonization in China

Deng Yuhang,Zhang Chao,Wang Jing,Zeng Jie,Zhang Jiangtao,Zhang Tianjiao,Zhao Haijian,Zhou Weiyan,Zhang Chuanbao 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.4

Background: Serum C-peptide results from various routine methods used in China are highly variable, warranting well-performing methods to serve as an accuracy base to improve the harmonization of C-peptide measurements in China. We developed an accurate isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC–MS/MS) method for serum C-peptide measurement and explored its use in harmonization. Methods: After protein precipitation with ZnSO4 solution, C-peptide was extracted from serum samples by anion-exchange solid-phase extraction and quantified by ID-LC–MS/MS in positive ion mode. The precision and analytical recovery of the ID-LC–MS/MS method were assessed. Seventy-six serum samples were analyzed using the ID-LC–MS/MS method and six routine immunoassays. Ordinary linear regression (OLR) and Bland-Altman (BA) analyses were conducted to evaluate the relationship between the ID-LC–MS/MS method and routine immunoassays. Five serum pool samples assigned using the ID-LC–MS/MS method were used to recalibrate the routine assays. OLR and BA analyses were re-conducted after recalibration. Results: The within-run, between-run, and total precision for the ID-LC–MS/MS method at four concentrations were 1.0%–2.1%, 0.6%–1.2%, and 1.3%–2.2%, respectively. The analytical recoveries for the ID-LC–MS/MS method at three concentrations were 100.3%–100.7%, 100.4%–101.0%, and 99.6%–100.7%. The developed method and the immunoassays were strongly correlated, with all R2 >0.98. The comparability among the immunoassays was substantially improved after recalibration. Conclusions: The performance of the ID-LC–MS/MS method was carefully validated, and this method can be used to improve the harmonization of serum C-peptide measurements in China.

Potential molecular mechanisms underlying the effect of arsenic on angiogenesis

Juan Zhang,Yue Zhang,Weiyan Wang,Zhiyi Zhang 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.11

Arsenic is a potent chemotherapeutic drug thatis applied as a treatment for cancer; it exerts its functionsthrough multiple pathways, including angiogenesis inhibition. As angiogenesis is a critical component of the progressionof many diseases, arsenic is a feasible treatmentoption for patients with other angiogenic diseases, includingrheumatoid arthritis and psoriasis, among others. However,arsenic is also a well-known carcinogen, demonstrating apro-angiogenesis effect. This review will focus on the dualeffects of arsenic on neovascularization and the relevantmechanisms underlying these effects, aiming to provide arational understanding of arsenic treatment. In particular, weexpect to provide a comprehensive overview of the currentknowledge of the mechanisms by which arsenic influencesangiogenesis.

STYXL1 promotes proliferation and epithelial mesenchymal transition of gastric cancer cells via activating the PI3K/AKT pathway

Chen Silu,Yu Weiyan,Li Ziyue,Wang Yadong,Peng Bo 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Background Gastric cancer (GC) is a common and grievous disorder with high heterogeneity. Serine/threonine/tyrosine-interacting-like protein 1 (STYXL1), a pseudophosphatase without catalytic activity, plays a important roles in cellular pathways and various cancers. However, its role and mechanism in GC remain unclear. Objectives This study aimed to explore the role and possible mechanism of STYXL1 in GC cells. Results The results showed that STYXL1 expression was elevated in GC. Both loss- and gain-of-function results showed that STYXL1 enhanced cell viability, colony formation, cell invasion and migration, and the protein expression of BCL-2 and Vimentin, but reduced the apoptosis rate and the protein level of BAX, cleaved caspase 3 and E-Cadherin in vitro. Mechanically, the levels of p-PI3K/PI3K and p-AKT/AKT were observably elevated by overexpression of STYXL1, and markedly reduced by silencing of STYXL1 in both SNU-1 and HGC-27 cells. Conclusion STYXL1 promoted cell growth, migration, invasion and EMT with decreased apoptosis, which was closely related with the activation of PI3K/AKT pathway in GC.

PREPARATION AND CHARACTERIZATION OF SILVER NANOPARTICLES/CARBON NANOFIBERS VIA ELECTROSPINNING WITH RESEARCH ON THEIR CATALYTIC PROPERTIES

Huan Liu,Jie Bai,Chunping Li,Shan Wang,Yarong Huang,QI WANG,WEIYAN SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.3

Ag nanoparticles (Ag NPs) embedded carbon nano¯bers (CNFs) were prepared by a new routewhich included chemical reaction process, electrospinning and calcination technique. The mor-phology and structure of the composite nano¯bers were investigated by scanning electron mi-croscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy and X-raydi®raction. It indicated that Ag NPs were uniformly distributed in the CNFs. This e®ectivesynthesis method can be used to prepare other composite nano¯bers with functionality. The AgNPs/CNFs that served as supported catalysts were used in the styrene epoxidation by TBHP. The Ag NPs/CNFs catalyst showed its highly catalytic activity for the epoxidation of styrene(conversion: 40.6%, SO selectivity: 35.9%). This kind of composite nano¯ber membrane wasproved e®ectively catalytic activity and recycled easily in the styrene epoxidation.

Interleukin-6 signaling regulates hematopoietic stem cell emergence

Ruxiu Tie,Honghu Li,Shuyang Cai,Zuyu Liang,Wei Shan,Binsheng Wang,Yamin Tan,Weiyan Zheng,He Huang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Hematopoietic stem cells (HSCs) produce all lineages of mature blood cells for the lifetime of an organism. Invertebrates, HSCs derive from the transition of the hemogenic endothelium (HE) in the floor of the embryonic dorsalaorta. Most recently, a series of proinflammatory factors, such as tumor necrosis factor-α, interferon-γ, and Toll-likereceptor 4, have been confirmed to play a key role in HSC specification. However, the full complement of necessarysignaling inputs remains unknown to date. Here, we show that interleukin-6R (IL6R) via IL6 is required and sufficient forHSC generation. We found that Notch activates IL6R by regulating its expression in the HE and in HSCs. The secretionof IL6 mainly originates from HSC-independent myeloid cells, but not from HSCs and their adjacent vascularendothelial cells. In addition, blocking IL6 signaling does not affect vascular development or the production ofprimitive erythrocytes. Taken together, our results uncover a previously obscure relationship between IL6 signaling andHSC production and provide new insights into HSC regeneration using proinflammatory factors in vitro.

Effects of short-term soil tillage management on activity and community structure of denitrifiers under double-cropping rice field

Haiming Tang,Chao Li,Kaikai Cheng,Lihong Shi,Li Wen,Xiaoping Xiao,Yilan Xu,Weiyan Li,Ke Wang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.11

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH4 +-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.