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RISS 인기검색어
- 검색키워드
- 저자 : Vimarsh Raina (검색결과 2 건)
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Stability of eosin-5'-maleimide dye used in flow cytometric analysis for red cell membrane disorders
Simmi Mehra,Neetu Tyagi,Pranav Dorwal,Amit Pande,Dharmendra Jain,Ritesh Sachdev,Vimarsh Raina 대한혈액학회 2015 Blood Research Vol.50 No.2
BackgroundThe eosin-5'-maleimide (EMA) binding test using flow cytometry is a common methodto measure reduced mean channel fluorescence (MCF) of EMA-labeled red blood cells(RBCs) from patients with red cell membrane disorders. The basic principle of theEMA-RBC binding test involves the covalent binding of EMA to lysine-430 on the first extracellularloop of band 3 protein. MethodsIn the present study, the MCF of EMA was analyzed for samples derived from 12 healthyvolunteers (controls) to determine the stability (i.e., the percentage decrease in fluorescence)of EMA over a period of 1 year. ResultsComparison of periodical MCF readings over time, that is, at 2-month intervals, showedthat there were no significant changes in mean channel fluorescence for up to 6 months;however, there was a significant decrease in MCF at 8 months. ConclusionFor optimal dye utilization, EMA remained stable only for up to 6 months. Therefore, werecommend reconstitution of the dye every 6 months when implementing this test andstorage at -80oC in dark conditions.
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Stability of eosin-5'-maleimide dye used in flow cytometric analysis for red cell membrane disorders
Simmi Mehra,Neetu Tyagi,Pranav Dorwal,Amit Pande,Dharmendra Jain,Ritesh Sachdev,Vimarsh Raina 대한혈액학회 2015 Blood Research Vol.50 No.2
BackgroundThe eosin-5'-maleimide (EMA) binding test using flow cytometry is a common methodto measure reduced mean channel fluorescence (MCF) of EMA-labeled red blood cells(RBCs) from patients with red cell membrane disorders. The basic principle of theEMA-RBC binding test involves the covalent binding of EMA to lysine-430 on the first extracellularloop of band 3 protein. MethodsIn the present study, the MCF of EMA was analyzed for samples derived from 12 healthyvolunteers (controls) to determine the stability (i.e., the percentage decrease in fluorescence)of EMA over a period of 1 year. ResultsComparison of periodical MCF readings over time, that is, at 2-month intervals, showedthat there were no significant changes in mean channel fluorescence for up to 6 months;however, there was a significant decrease in MCF at 8 months. ConclusionFor optimal dye utilization, EMA remained stable only for up to 6 months. Therefore, werecommend reconstitution of the dye every 6 months when implementing this test andstorage at -80oC in dark conditions.
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