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Facile conversion of ATP-binding RNA aptamer to quencher-free molecular aptamer beacon
Park, Yoojin,Nim-anussornkul, Duangrat,Vilaivan, Tirayut,Morii, Takashi,Kim, Byeang Hyean Pergamon Press 2018 Bioorganic & medicinal chemistry letters Vol.28 No.2
<P><B>Abstract</B></P> <P>We have developed RNA-based quencher-free molecular aptamer beacons (RNA-based QF-MABs) for the detection of ATP, taking advantage of the conformational changes associated with ATP binding to the ATP-binding RNA aptamer. The RNA aptamer, with its well-defined structure, was readily converted to the fluorescence sensors by incorporating a fluorophore into the loop region of the hairpin structure. These RNA-based QF-MABs exhibited fluorescence signals in the presence of ATP relative to their low background signals in the absence of ATP. The fluorescence emission intensity increased upon formation of a RNA-based QF-MAB·ATP complex.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>