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- 저자 : Vidhi Chaudhary (검색결과 2 건)
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Green synthesis of fluorescent carbon nanoparticles from Lychee (Litchi chinensis) plant
Vidhi Chaudhary,Anil Kumar Bhowmick 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.8
Fluorescent carbon nanoparticles (CNPs) were prepared from waste lychee peel by hydrothermal process using green chemistry. These CNPs were characterized by UV-vis absorption, fluorescence spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction. Their morphology was analyzed by field emission scanning electron microscopy. Spherical carbon nanoparticles with a particle size ranging from 40-70 nm were observed. FTIR data indicated that these CNPs were functionalized with hydroxyl and carboxylic or carbonyl group. The maximum fluorescence intensity for these CNPs was observed on the excitation wavelength at 365 nm with emission maxima centered at 450 nm. These particles exhibited excitation wavelength dependent fluorescence emission spectra. CNPs were found to be highly fluorescent and exhibited high water solubility. The band gap was estimated to be 3.8 eV. Therefore, as prepared CNPs would be useful in bioimaging, biolabeling and in the other applications of nanobiotechnology.
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Singh, Jay,Roychoudhury, Appan,Srivastava, Manish,Chaudhary, Vidhi,Prasanna, Radha,Lee, Dong Won,Lee, Seung Hee,Malhotra, B. D. American Chemical Society 2013 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.117 No.16
<P>A new hybrid nanocomposite based on hydrothermally synthesized nanostructured NiFe<SUB>2</SUB>O<SUB>4</SUB> (<I>n</I>-NiFe<SUB>2</SUB>O<SUB>4</SUB>) and chitosan (CH) has been explored for bienzyme (cholesterol esterase (ChEt) and cholesterol oxidase (ChOx)) immobilization for application as total cholesterol biosensor. Results of X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), Raman spectroscopy (RS) and vibrating sample magnetometer (VSM) studies demonstrate that the ChEt–ChOx/<I>n</I>-NiFe<SUB>2</SUB>O<SUB>4</SUB>–CH composite film is successfully synthesized. The obtained ChEt–ChOx/<I>n</I>-NiFe<SUB>2</SUB>O<SUB>4</SUB>–CH nanocomposite film shows large specific area, high conductivity, good biocompatibility, fast redox properties and improved antimicrobial activity. The fabricated ChEt–ChOx/<I>n</I>-NiFe<SUB>2</SUB>O<SUB>4</SUB>–CH/ITO bioelectrode exhibits largely improved amperometric biosensing performance, i.e., good linearity (5–400 mg/dL), low detection limit (24.46 mg/dL cm<SUP>–2</SUP>), high sensitivity of 1.73 μA/(mg/dL cm<SUP>–2</SUP>), fast response time of 15s, reproducibility of more than 15 times, shelf life of about 90 days and low Michaelis–Menten constant (<I>K</I><SUB><I>m</I></SUB>) value as 7.05 mg/dL (0.1825 mM). Furthermore, this modified bioelectrode has been utilized for estimation of total cholesterol in human serum samples. This efficient strategy provides new insight into the design of novel flexible electrodes for a wide range of applications in biosensing, bioelectronics, and clinical applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2013/jpccck.2013.117.issue-16/jp312698g/production/images/medium/jp-2012-12698g_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp312698g'>ACS Electronic Supporting Info</A></P>
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