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Loop-mediated Isothermal Amplification (LAMP)법을 이용한 Chronic Bee Paralysis Virus (CBPV)의 신속 진단법 개발
노지나(Ji-Na No),유미선(Mi-Sun Yoo),Van Phu Nugyen,윤병수(Byoung Su Yoon) 한국양봉학회 2010 韓國養蜂學會誌 Vol.25 No.4
Chronic bee paralysis virus (CBPV) is the causal agent of chronic paralysis in honeybee colonies. A loop-mediated isothermal amplification (LAMP) assay allows one-step detection of gene amplification by simple reaction and requires only a simple incubator, such as water bath providing a constant temperature. In this study, CBPV-LAMP was developed for detection of CBPV. The four CBPVspecific primers named CBPV-LAMP-F3/B3/FIP/BIP were designed. CBPV was successfully amplified under isothermal conditions at about 54°C within 30 minutes and CBPV-LAMP could be detect untill 1×10⁴ copies. Especially, developed method, CBPV-LAMP was direct detection analysis by the naked eye without electrophoresis using SYBR Green I. This method provides an important diagnostic tool for the detection of CBPV infection.