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RISS 인기검색어
- 검색키워드
- 저자 : Udayakumar Rajangam (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Gopalakrishnan Karikalan,Udayakumar Rajangam 한국약제학회 2018 Journal of Pharmaceutical Investigation Vol.48 No.4
Marsilea quadrifolia is an indigenous medicinal plant and it has a folk reputation in central and southern India as hypoglycemic agent. The present study was aimed to evaluate the effect of methanolic leaf and stem extracts of Marsilea quadrifolia on carbohydrate metabolic enzymes in alloxan induced diabetic rats. In this study, the blood glucose level was increased in alloxan induced diabetic rats when compared to normal control rats. The decreased level of blood glucose was observed in diabetic rats treated with leaf and stem of M. quadrifolia. The activities of hexokinase, pyruvate kinase, lactate dehydrogenase, glucose-6-phosphatase, fructose 1,6-bisphosphatase and glucose-6-phosphate dehydrogenase in liver and kidney and glycogen content, glycogen synthase and glycogen phosphorylase in liver were analysed. Glibenclamide was used as a reference drug in the present investigation. The activities of hexokinase, pyruvate kinase and glucose- 6-phosphate dehydrogenase were significantly decreased and the activities of lactate dehydrogenase, glucose-6-phosphatase and fructose 1,6-bisphosphatase were significantly increased in liver and kidney of alloxan induced diabetic rats when compared to normal control rats. Oral administration of methanolic leaf and stem extracts of M. quadrifolia (300 mg/kg bw) to diabetic rats for 45 days showed significantly reverted levels of above mentioned enzymes. The levels of glycogen and glycogen synthase in liver were significantly decreased and the glycogen phosphorylase activity was significantly increased in diabetic rats when compared to normal control rats. The diabetic rats treated with leaf and stem extracts of M. quadrifolia reverted the levels of glycogen, glycogen synthase and glycogen phosphorylase to near normal. The present study clearly indicates that the methanolic leaf and stem extracts of M. quadrifolia possesses potent antidiabetic activity.
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Antioxidant Activity of Datura alba During Wound Healing Process
( Ramamourthy Gopa1 ),( Cbandrasekaran Gayathri ),( Rajangam Udayakumar ),( Thulasingam Senthilkumar ) 조선대학교 공학기술연구원 2011 공학기술논문지 Vol.4 No.2
Datura alba, a traditional Indian folk medicinal plant. The present study aims to evaluate the antioxidant property of this plant and its involvement in the regulation of matrix metalloproteinase (MMPs) 0 animal bum wound healing models. The level of antioxidant (vitamin C) was higher in the treated group than in the control group. Generation of malonaldehyde was reduced b the presence of secondary metabolites of the plant extract applied topically. Both pro and active forms of MMP2 and MMP9 were predominant during the process of healing. Significant increase in the synthesis of collagen was observed in the treated group. Therefore, the wound healing activity of the extract may be due to its antioxidant effect, which is a free radical scavenger that promotes healing.
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Lee, Young Seok,Choi, Chang Won,Kim, Jae Jin,Ganapathi, Andy,Udayakumar, Rajangam,Kim, Sei Chang Molecular Diversity Preservation International (MD 2009 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.10 No.1
<P>Safflower (<I>Carthamus tinctorius</I> L.) seeds are used as a folk medicine to enhance bone formation or to prevent osteoporosis in Korea. Therefore, the methanolic extract of safflower seeds (MESS) containing high mineral content, such as calcium (Ca), potassium (K) and phosphorous (P), was evaluated for the role on osteoblast (Ob) markers of Sprague-Dawley rats. In serum of 3 to 11 weeks (wks) old rats, both osteocalcin (OC) content and bone-specific alkaline phosphatase (B-ALP) activity increased to their maximum levels in 4–7 wks. Hence, 3 wks old rats were selected for 8 wks oral treatment of MESS, resulted in the significant increase of Ob markers in serum such as OC content (4–8 wks), B-ALP activity (1–2 wks) and insulin-like growth factor I (IGF-I) level (1 wk), and the growth parameter such as the length of femur (2–8 wks) and tibia (4 wks). On the basis of Pearson’s correlation coefficient, there were a moderate correlation between OC and B-ALP at 8 wks, a low correlation between OC and IGF-I at 1, 4 and 8 wks, a moderate correlation between OC and femur length at 1, 2 and 8 wks, and a moderate correlations between OC and tibia length at 1 and 8 wks of MESS-treated groups. The result reveals that the changes of OC correlated at low to moderate level with the changes of B-ALP activity, IGF-I content and femur and tibia length in the MESS-treatment period. On the other hand, there were a strong correlation between IGF-I and femur length at 2 wks and moderate correlation between IGF-I and tibia length at 1, 2 and 8 wks of MESS-treated groups. Therefore, the effect of MESS on bone formation likely appears to be mediated by IGF-I at the early stage of treatment.</P>
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Oh, Tae-Kyun,Oh, Sung,Kim, Seongdae,Park, Jae Sung,Vinod, Nagarajan,Jang, Kyung Min,Kim, Sei Chang,Choi, Chang Won,Ko, Suk-Min,Jeong, Dong Kee,Udayakumar, Rajangam MDPI 2014 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.15 No.9
<P>A full-length phytase gene (<I>phy</I>) of <I>Aspergillus nidulans</I> was amplified from the cDNA library by polymerase chain reaction (PCR), and it was introduced into a bacterial expression vector, pET-28a. The recombinant protein (rPhy-E, 56 kDa) was overexpressed in the insoluble fraction of <I>Escherichia coli</I> culture, purified by Ni-NTA resin under denaturing conditions and injected into rats as an immunogen. To express <I>A</I><I>. nidulans</I> phytase in a plant, the full-length of <I>phy</I> was cloned into a plant expression binary vector, pPZP212. The resultant construct was tested for its transient expression by <I>Agrobacterium</I>-infiltration into <I>Nicotiana benthamiana</I> leaves. Compared with a control, the agro-infiltrated leaf tissues showed the presence of <I>phy</I> mRNA and its high expression level in <I>N.</I><I>benthamiana</I><I>.</I> The recombinant phytase (rPhy-P, 62 kDa) was strongly reacted with the polyclonal antibody against the nonglycosylated rPhy-E. The rPhy-P showed glycosylation, two pH optima (pH 4.5 and pH 5.5), an optimum temperature at 45~55 °C, thermostability and broad substrate specificities. After deglycosylation by peptide-<I>N</I>-glycosidase F (PNGase-F), the rPhy-P significantly lost the phytase activity and retained 1/9 of the original activity after 10 min of incubation at 45 °C. Therefore, the deglycosylation caused a significant reduction in enzyme thermostability. In animal experiments, oral administration of the rPhy-P at 1500 U/kg body weight/day for seven days caused a significant reduction of phosphorus excretion by 16% in rat feces. Besides, the rPhy-P did not result in any toxicological changes and clinical signs.</P>
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