http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Mosadegh, Bobak,Agarwal, Mayank,Tavana, Hossein,Bersano-Begey, Tommaso,Torisawa, Yu-suke,Morell, Maria,Wyatt, Matthew J.,O'Shea, K. Sue,Barald, Kate F.,Takayama, Shuichi Royal Society of Chemistry 2010 Lab on a chip Vol.10 No.21
<P>Generation of stable soluble-factor gradients in microfluidic devices enables studies of various cellular events such as chemotaxis and differentiation. However, many gradient devices directly expose cells to constant fluid flow and that can induce undesired responses from cells due to shear stress and/or wash out of cell-secreted molecules. Although there have been devices with flow-free gradients, they typically generate only a single condition and/or have a decaying gradient profile that does not accommodate long-term experiments. Here we describe a microdevice that generates several chemical gradient conditions on a single platform in flow-free microchambers which facilitates steady-state gradient profiles. The device contains embedded normally-closed valves that enable fast and uniform seeding of cells to all microchambers simultaneously. A network of microchannels distributes desired solutions from easy-access open reservoirs to a single output port, enabling a simple setup for inducing flow in the device. Embedded porous filters, sandwiched between the microchannel networks and cell microchambers, enable diffusion of biomolecules but inhibit any bulk flow over the cells.</P> <P>Graphic Abstract</P><P>We describe a microdevice that simultaneously generates several gradient conditions in flow-free microchambers separated by normally-closed valves that enable efficient cell seeding. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0lc00086h'> </P>
High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array
Tung, Yi-Chung,Hsiao, Amy Y.,Allen, Steven G.,Torisawa, Yu-suke,Ho, Mitchell,Takayama, Shuichi Royal Society of Chemistry 2011 The Analyst Vol.136 No.3
<P>Culture of cells as three-dimensional (3D) aggregates can enhance <I>in vitro</I> tests for basic biological research as well as for therapeutics development. Such 3D culture models, however, are often more complicated, cumbersome, and expensive than two-dimensional (2D) cultures. This paper describes a 384-well format hanging drop culture plate that makes spheroid formation, culture, and subsequent drug testing on the obtained 3D cellular constructs as straightforward to perform and adapt to existing high-throughput screening (HTS) instruments as conventional 2D cultures. Using this platform, we show that drugs with different modes of action produce distinct responses in the physiological 3D cell spheroids compared to conventional 2D cell monolayers. Specifically, the anticancer drug 5-fluorouracil (5-FU) has higher anti-proliferative effects on 2D cultures whereas the hypoxia activated drug commonly referred to as tirapazamine (TPZ) are more effective against 3D cultures. The multiplexed 3D hanging drop culture and testing plate provides an efficient way to obtain biological insights that are often lost in 2D platforms.</P> <P>Graphic Abstract</P><P>A 384-well format hanging drop culture plate that enables spheroid formation, culture, and drug testing using existing high-throughput screening instruments. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0an00609b'> </P>