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Pisciglobus halotolerans gen. nov., sp. nov., isolated from fish sauce.
Tanasupawat, Somboon,Thongsanit, Jaruwan,Thawai, Chitti,Lee, Keun Chul,Lee, Jung-Sook Society for General Microbiology 2011 International journal of systematic and evolutiona Vol.61 No.7
<P>Two strains of Gram-stain-positive, catalase-negative, tetrad-forming cocci, C01(T) and C02, were isolated in Thailand from fish sauce. They were facultatively anaerobic, non-motile and non-spore-forming bacteria. These strains produced l-lactic acid from glucose. They grew at pH 5.0-9.0, at 15-40 °C and in the presence of 10???% (w/v) NaCl. The dominant fatty acid was C(18???:???1)??9c. The DNA G+C contents of strains C01(T) and C02 were 38.6 and 38.7 mol%, respectively. Strain C01(T) was related most closely to Desemzia incerta DSM 20581(T), with a 16S rRNA gene sequence similarity of 96.9???%. The strains could be distinguished clearly from D. incerta DSM 20581(T) based on cell morphology, physiological and biochemical characteristics and low levels of DNA-DNA relatedness. On the basis of the data presented, strains C01(T) and C02 are considered to represent a novel species of a new genus in the Bacillus-Lactobacillus cluster, for which the name Pisciglobus halotolerans gen. nov., sp. nov. is proposed. The type strain of Pisciglobus halotolerans is C01(T) (???=???KCTC 13150(T) ???=???TISTR 1958(T) ???=???PCU 316(T)).</P>