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RISS 인기검색어
- 검색키워드
- 저자 : Tamotsu OOTAKI (검색결과 3 건)
- 무료
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INVITED REVIEW - PHOTOTROPISM OF PHYCOMYCES SPORANGIOPHORES
Ootaki, Tamotsu Korean Society of Photoscience 1994 Journal of Photosciences Vol.1 No.1
Sporangiophores (spphs) of Phycomyces blakesleeanus are positively phototropic to unilateral visible (blue) light over a range of fluence rates 10$^{-9}$ to 1 W/m$^2$. The maximal bending angle is always 70-75$\circ$ from the vertical. Many mutants with abnormal phototropism have been isolated. Complementation tests revealed that the genetic grouping is completely consistent with the phenotypic classification scheme, based on sensory responses other than those to light. The spph of the piloboloid mutant, the growth zone of which gradually ceases elongation but expands spherically, and the $\beta$-carotene-overproducing mutant show negative phototropism, in contrast to the wild type spph. We hypothesized that the phototropic orientation of spph is determined by the ratio of the maximal light fluenee rate at the proximal side to that at the distal side of the spph. Based on this hypothesis, we found that the maximal bending angle was larger in thin spphs than in thick ones, and larger in spphs containing smaller amount of $\beta$-carotene than in carotene-rich spphs. In addition to our hypothesis, gravitropic experiments revealed that the maximal bending angle of the wild type spph results from a balance among positive phototropism, negative gravitropism, and the optical properties of the spph. For further advancement of this study, we developed a mutant with a high proportion of uninucleate spores, and designed an efficient microinjection method for obtaining transformants.
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Analysis of the Formation of Protoplasts and Regeneration of Cells in Phycomyces blakesleeanus
Fukui, Joe,Choi, Kwan Sam,Miyazaki, Atsushi,Ootaki, Tamotsu,Oikawa, Taneaki 한국미생물 · 생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.1
It is possible to prepare protoplasts of the zygomycete fungus, Phycomyces blakesleeanus, by digesting the cell wall of spore germlings with commercially available chitinase and chitosanase. However, the cells without any cell walls immediately form large aggregates, and thus, it is difficult to isolate the individually separated protoplasts. Inherent problem with the formation of aggregates in preparing protoplasts could be solved by the use of bovine serum albumin (BSA). As a result, we were able to prepare a large number of single protoplasts quickly and easily. We took time-lapse photomicrographs of the formation of protoplasts, and found that there were certain regions of the cell wall of spore germlings that were sensitive to chitinase and chitosanase, although the cell wall of the original spores is known to be insensitive to these enzymes. There are two kinds of cell walls on a spore germling; one with a bound wheat germ agglutinin (WGA), and the other a bound concanavalin A (ConA). Furthermore, only cells with walls which had bound WGA were able to regenerate, while those with walls with bound ConA were not able to regenerate.
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