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Transgenesis in Fish: Indian Endeavour and Achievement
Pandian, T.J The Korean Society of Fisheries and Aquatic Scienc 2003 韓國養殖學會誌 Vol.16 No.1
The first Indian transgenic fish was generated in 1991 using borrowed constructs from foreign sources. To construct transformation vectors for the indigenous fishes, growth hormone genes of rohu (r-CH), Labeo rohita and catfish, Heteropneustes fossilis were isolated, cloned and sequenced; their fidelity was confirmed in prokaryotic and eukaryotic systems. A vector was constructed with grass carp b-actin promoter driving the expression of r-GH. Rohu eggs are large. fragile and swell 2~3 times. when fertilized. Hence they were amenable only for electroporated sperm-mediated gene transfer. Accordingly, the sperm electroporation technique was standardized to ensure 25% hatchling survival and 37% Presumptive transgenics without suffering any deformity. Southern analysis confirmed genomic integration in 15% of the tested individuals (Ti) belonging to family lines 2 and 3: another 25% of the Juveniles (Te) were also proved transgenic but with the transgene persisting extrachromosomally for longer than 1 to 2 years. perhaps due to the presence of replicon in the vector. Transgenics belonging to different family lines grew 6~8 times faster than the respective controls. Difference in growth trends of Ti and Te within a family line was not significant. In the Ti family 3 remarkable growth acceleration was sustained for a period longer than 36 weeks but in those of family 2, it gradually decreased. All transgenic fishes including the rohu converted the food at a significantly higher efficiency. Barring the transgenic mudloach, all the other transgenic fishes consumed food at significantly reduced rate.
Asian Aquaculture Journals: Problems and Possibilities
Pandian, T.-J. The Korean Society of Fisheries and Aquatic Scienc 2001 韓國養殖學會誌 Vol.14 No.1
Global status of aquaculture and aquaculture scientific journals is briefly described. Asia is the centre of fishing and aquaculture activities but most publishers, editors and editorial board members of fisheries and aquaculture journals are from Europe and/or USA. The contribution by Asian fisheries/aquaculture scientists to these journals remains low. Language barrier and requirement for monitory investment seem to inhibit Asian participation in this realm of scientific activity. The need for glob-alization of aquaculture scientific journals is emphasized. Selected strategies, which are adopted by the Western journals to improve their image and management, are indicated.
Effect of Tributyltin Chloride on Survival, Growth and Reproduction in Zebra Fish Danio rerio
Balasubramani, A.,Pandian, T.J. The Korean Society of Fisheries and Aquatic Scienc 2008 韓國養殖學會誌 Vol.21 No.3
Tributyltin chloride(TBTCl) was administered through discrete immersion(2 hr each) from the $18^{th}-25^{th}$ day after hatching(dph). At the doses of 1, 2, 4 and 8 ${\mu}g/L$, the immersion at 2 ${\mu}g/L$ ensured 93% masculinization and the highest survival of 75% after the treatment. TBTCl acted as a growth suppressant and the magnitude of its suppression was stronger in females. During the 300 day experiment, it postponed sexual maturity of females from 120$^{th}$ dph in the control to 240$^{th}$ dph in the females treated at 8 ${\mu}g/L$. It reduced spawning frequency(22-3 times) and cumulative fecundity(1,632-19 eggs) by reducing the number of vitellogenic eggs. In the treated males too, it reduced sperm motility(100-68 sec); consequently, fertilizability of the sperm cells drawn from these males was also reduced from 88 to 43%. Progeny testing showed that the cross between males treated at>2 ${\mu}g/L$ and normal females generated the presumed 'homogametic' males. Both the treated 'homogametic' and 'heterogametic' males could induce the females to spawn fewer eggs than that of the normal males. A normal female somehow deducted the differences between the control, treated and sex reversed males; it preferred a normal male over a treated one, and a treated one over the sex reversed male.