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- 저자 : Sun Yaqiong (검색결과 2 건)
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Gene expression profile of NFκB repressing factor(NKRF) knockdown cells by microarray analysis
Sun Yaqiong,Zheng Dan,Gu Shaohua,Mao Yumin,Xie Yi 한국바이오칩학회 2012 BioChip Journal Vol.6 No.3
Human NFκB repressing factor(NKRF) is a negative regulation transcription factor, which is able to repress transcription by binding to the negative regulatory element(NRE) near the NFκB binding site in certain genes’ promoters. Current researches reveals that NKRF represses the activation of IFN-β, IL-8,hiNOS and HIV-1 by NFκB. We used optical fiber beadchip to analysis the different gene expression patterns of RNAi mediated NKRF knockdown HEK293 cells and found that several genes showed significant change of expression levels. Real-time PCR was performed to verify the changes of expression of candidate genes. We analyzed the function of candidate genes by searching the gene ontology databases and publications and revealed that these genes functioned in cell cycle, cell proliferation, apoptosis, cell migration, DNA repair, transcription, metabolism, response to stimulus and signal transduction. This study provides new perspectives on NKRF’s potential multiple functions.
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Preparation and characterization of a porous silicate material from silica fume
Yinmin Zhang,Haiping Qi,Yaqiong Li,Yongfeng Zhang,Junmin Sun 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.12
A porous silicate material derived from silica fume was successfully prepared and characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared (FT-IR) spectroscopy, Thermogravimetry and Differential thermal gravity (TG-DTG), N2 adsorption and desorption isotherms, and scanning electron microscopy (SEM). Raw silica fume was analyzed by XRD, FT-IR and SEM. The analysis results of silica fume indicated that SiO2 in silica fume is mainly determined as amorphous state, and that the particles of raw silica fume exhibited characteristic spherical structure with a diameter of from 50 nm to 200 nm. The preparation of the porous silicate material involved two steps. The first step was the extraction of the SiO3 2− leachate from raw silica fume. The maximum value of SiO3 2− extraction yield was obtained under the following conditions: reaction temperature of 120 oC, reaction time of 120 min, NaOH concentration of 15%, and alkali to SiO2 molar ratio of 2. The second step was the preparation of the porous silicate material though the reaction of SiO3 2− leachate and Ca(OH)2 suspension liquid. The optimum preparation conditions were as follows: preparation temperature of 90 oC, preparation time of 1.5 h, Si/Ca molar ratio of 1 : 1, and stirring rate of 100 r/min. The BET surface area and pore size of the porous silicate material were 220.7m2·g−1 and 8.55 cm3/g, respectively. The porous silicate material presented an amorphous and unordered structure. The spectroscopic results indicated that the porous silicate material was mainly composed of Si, Ca, O, C, and Na, in the form of Ca2+, SiO3 2−, CO3 2− and Na+ ions, respectively, which agreed with the XRD, TG-DSC, and FT-IR data. The N2 adsorption-desorption isotherm mode indicates that the porous silicate material belonged to a typical mesoporous material. The porous silicate material presented efficiency for the removal of formaldehyde: it showed a formaldehyde adsorption capacity of 8.01mg/g for 140 min at 25 oC.
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