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Sana Sheik,K. R. Chandrashekar,Sukesh Shirlal 경희대학교 융합한의과학연구소 2018 Oriental Pharmacy and Experimental Medicine Vol.18 No.2
Psychotria flavida Talbot. an endemic plant of Western Ghats was explored for endophytic mycoflora. The endophytic fungi isolated from leaf and stem were identified with the aid of morphology and molecular methods. Lasiodiplodia theobromae and Picchia guillermondii were commonly present in both the sites. Overall colonization frequency of leaf samples was found to be higher than stem samples in both the places. Studies on seasonal variation of the occurrence of these fungi indicated a higher frequency during winter as compared to monsoon and summer. Further, endophytic fungi were verified for bioactive diagnosis using in vitro antimicrobial (disc diffusion method) and antioxidant (DPPH free radical scavenging). Among the 15 crude extracts of different endophytes tested, the extracts of Lasiodiplodia theobromae possessed potentially better antibacterial and antioxidant activity. The present study divulges that the endophytic fungi L. theobromae could be a potential source of antimicrobial and antioxidant drugs.